Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	128	54.7	1845674	99.3%	1832754	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,967,846	C→T	V197M (GTG→ATG)	ptsP ←	phosphoenolpyruvate‑protein phosphotransferase PtsP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,967,846	0	C	T	100.0%	158.8 / NA	46	V197M (GTG→ATG)	ptsP	phosphoenolpyruvate‑protein phosphotransferase PtsP
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/25); total (21/25)

AAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGC  >  NC_000913/2967710‑2967981

aaaaTAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTg                                                                                                                                      >  2:360755/1‑140 (MQ=255)

 aaaTAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGt                                                                                                                                     >  1:607190/1‑140 (MQ=255)

  aaTAGCCGCCATTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGtt                                                                                                                                    <  2:796556/140‑1 (MQ=255)

      gccgccGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCAt                                                                                                                                >  2:360230/1‑140 (MQ=255)

      gccgccGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCAt                                                                                                                                >  1:530694/1‑140 (MQ=255)

       ccgccgTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCAtt                                                                                                                               >  2:276955/1‑140 (MQ=255)

             tttCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGt                                                                                                                         <  2:835514/140‑1 (MQ=255)

                      gtgCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCa                                                                                                                <  1:536272/140‑1 (MQ=255)

                       tgCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCAt                                                                                                               >  1:340322/1‑140 (MQ=255)

                           ccGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTg                                                                                                           >  1:53741/1‑140 (MQ=255)

                                 gcAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGcc                                                                                                     >  2:521956/1‑140 (MQ=255)

                                 gcAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGcc                                                                                                     >  1:464740/1‑140 (MQ=255)

                                     aGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCg                                                                                                 <  2:209390/140‑1 (MQ=255)

                                        gTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCa                                                                                              <  1:360755/140‑1 (MQ=255)

                                           tGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATTCATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATc                                                                                           >  2:184687/1‑140 (MQ=255)

                                           tGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATc                                                                                           <  1:521956/140‑1 (MQ=255)

                                                tAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCcac                                                                                      >  2:407062/1‑140 (MQ=255)

                                                 aGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCcaca                                                                                     <  2:676500/140‑1 (MQ=255)

                                                       gAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGt                                                                               <  1:582872/140‑1 (MQ=255)

                                                       gAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGt                                                                               >  2:545018/1‑140 (MQ=255)

                                                         aaCTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGtgc                                                                             <  2:340322/140‑1 (MQ=255)

                                                         aaCTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGtgc                                                                             <  2:607190/140‑1 (MQ=255)

                                                                    cGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATg                                                                  >  1:350643/1‑140 (MQ=255)

                                                                      cttcttCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATgcg                                                                <  2:860429/140‑1 (MQ=255)

                                                                               gcgcCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCTGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTcgc                                                       >  2:852216/1‑140 (MQ=255)

                                                                               gcgcCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTcgc                                                       <  2:350643/140‑1 (MQ=255)

                                                                                 gcCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGt                                                     >  1:371008/1‑140 (MQ=255)

                                                                                         cAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGat                                             <  1:812265/140‑1 (MQ=255)

                                                                                         cAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGat                                             <  1:779359/140‑1 (MQ=255)

                                                                                          aGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGata                                            <  2:332993/140‑1 (MQ=255)

                                                                                            tCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATAtt                                          >  2:830809/1‑140 (MQ=255)

                                                                                              gTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGc                                        <  1:289248/140‑1 (MQ=255)

                                                                                                 cgcgTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATTTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCa                                     >  2:44531/1‑140 (MQ=255)

                                                                                                     ttCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACa                                 <  1:276955/140‑1 (MQ=255)

                                                                                                           agCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAg                           <  2:371008/140‑1 (MQ=255)

                                                                                                                gATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAAc                      >  1:783102/1‑140 (MQ=255)

                                                                                                                     aGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGTTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGa                 <  1:360230/140‑1 (MQ=255)

                                                                                                                      gCGTTGATGCCTGATACATTTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGAc                <  1:44531/140‑1 (MQ=255)

                                                                                                                      gCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGAc                <  2:464740/140‑1 (MQ=255)

                                                                                                                            aTGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGTGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGagaa          >  1:124007/1‑140 (MQ=255)

                                                                                                                            aTGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTGTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGagaa          >  2:860892/1‑140 (MQ=255)

                                                                                                                                 tGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAAt     >  2:254869/1‑140 (MQ=255)

                                                                                                                                   aTACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAg   <  1:741554/140‑1 (MQ=255)

                                                                                                                                   aTACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAg   <  1:545018/140‑1 (MQ=255)

                                                                                                                                   aTACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAg   <  1:54042/140‑1 (MQ=255)

                                                                                                                                    tACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGc  <  2:783102/140‑1 (MQ=255)

AAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGC  >  NC_000913/2967710‑2967981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A