Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	128	54.7	1845674	99.3%	1832754	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,999,755	(C)_6→7	coding (137/756 nt)	ygeR ←	LysM domain‑containing putative peptidase lipoprotein YgeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,999,749	1	.	C	100.0%	127.4 / NA	37	D48G (GAT→GGT)	ygeR	LysM domain‑containing putative peptidase lipoprotein YgeR
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (18/19); total (18/19)

GTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑CCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCC  >  NC_000913/2999614‑2999883

gtAATCCTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑ccc                                                                                                                                     <  2:685878/140‑1 (MQ=255)

gtAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑ccc                                                                                                                                     <  2:872912/140‑1 (MQ=255)

  aaTACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑ccccc                                                                                                                                   <  1:370643/140‑1 (MQ=255)

  aaTACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑ccccc                                                                                                                                   >  1:458561/1‑140 (MQ=255)

       tGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑cccccccgtt                                                                                                                              >  2:495213/1‑136 (MQ=255)

        gCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑cccccccgttt                                                                                                                             >  2:241527/1‑135 (MQ=255)

         cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTc                                                                                                                             >  1:516760/1‑140 (MQ=255)

         cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTc                                                                                                                             >  1:835716/1‑140 (MQ=255)

         cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTc                                                                                                                             >  2:568612/1‑140 (MQ=255)

          tACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCa                                                                                                                            <  2:201429/140‑1 (MQ=255)

          tACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCa                                                                                                                            <  1:106644/140‑1 (MQ=255)

                     ccacccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACg                                                                                                                 <  1:763140/140‑1 (MQ=255)

                      cacccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACgg                                                                                                                >  1:758795/1‑140 (MQ=255)

                       acccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGa                                                                                                               >  1:472447/1‑140 (MQ=255)

                        cccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAg                                                                                                              <  2:516760/140‑1 (MQ=255)

                             ttttAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGa                                                                                                         <  1:256745/140‑1 (MQ=255)

                              tttAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGaa                                                                                                        >  1:848075/1‑140 (MQ=255)

                                tAGTTTCTGACCATCTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa                                                                                                      <  2:195371/140‑1 (MQ=255)

                                tAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa                                                                                                      <  2:472447/140‑1 (MQ=255)

                                 aGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa                                                                                                      <  2:671382/139‑1 (MQ=255)

                                 aGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa                                                                                                      >  1:671382/1‑139 (MQ=255)

                                     tCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTc                                                                                                 <  1:219991/140‑1 (MQ=255)

                                                tCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGgat                                                                                      <  2:687468/140‑1 (MQ=255)

                                                      tgtaagggggggggAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTg                                                                                >  1:565202/8‑140 (MQ=255)

                                                       tgtAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAACTACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGc                                                                               <  2:801561/140‑1 (MQ=255)

                                                       tgtAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGc                                                                               <  2:458561/140‑1 (MQ=255)

                                                         taaggggggggAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTa                                                                             >  1:402366/5‑140 (MQ=255)

                                                              ggggggAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGa                                                                        >  1:583709/1‑140 (MQ=255)

                                                               gggggAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGaa                                                                       <  2:583709/140‑1 (MQ=255)

                                                                 gggAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACa                                                                     >  2:356708/1‑140 (MQ=255)

                                                                            ttCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCaaaa                                                          >  1:779720/1‑140 (MQ=255)

                                                                                  cgcgCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCTCGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTc                                                    <  2:583580/140‑1 (MQ=255)

                                                                                  cgcgCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTc                                                    <  1:492933/140‑1 (MQ=255)

                                                                                            cTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATaa                                          <  2:835716/140‑1 (MQ=255)

                                                                                               ttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATaacaa                                       <  1:241527/140‑1 (MQ=255)

                                                                                                        ttCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGAt                              <  1:495213/140‑1 (MQ=255)

                                                                                                             gtggtgCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCa                         <  2:779720/140‑1 (MQ=255)

                                                                                                                  gcgcGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGAt                    >  2:897510/1‑140 (MQ=255)

                                                                                                                   cgcgAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGAtt                   >  2:272786/1‑140 (MQ=255)

                                                                                                                   cgcgAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGAtt                   <  1:568612/140‑1 (MQ=255)

                                                                                                                                  aGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTc    >  1:759014/1‑140 (MQ=255)

                                                                                                                                  aGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTc    >  1:227003/1‑140 (MQ=255)

                                                                                                                                    cGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTccc  >  2:323695/1‑140 (MQ=255)

                                                                                                                                    cGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTccc  >  1:883735/1‑140 (MQ=255)

GTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑CCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCC  >  NC_000913/2999614‑2999883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A