Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	128	54.7	1845674	99.3%	1832754	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,322,459	A→G	L16P (CTT→CCT)	secG ←	Sec translocon subunit SecG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,322,459	0	A	G	100.0%	167.8 / NA	45	L16P (CTT→CCT)	secG	Sec translocon subunit SecG
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/20); total (25/20)

CGTTGCCAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAAGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGT  >  NC_000913/3322323‑3322593

cGTTGCCAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGc                                                                                                                                     >  2:745459/1‑140 (MQ=255)

cGTTGCCAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGc                                                                                                                                     >  1:69353/1‑140 (MQ=255)

     ccAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCACCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAAtt                                                                                                                                >  2:838523/1‑140 (MQ=255)

         cagcGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCca                                                                                                                            <  1:231882/140‑1 (MQ=255)

           gcgcCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCcaca                                                                                                                          >  1:38558/1‑140 (MQ=255)

            cgcCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACaa                                                                                                                         >  1:677914/1‑140 (MQ=255)

                    tGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGaaa                                                                                                                 <  1:221120/140‑1 (MQ=255)

                            aTGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTaa                                                                                                         >  2:847281/1‑140 (MQ=255)

                              gAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTaaaa                                                                                                       <  2:728038/140‑1 (MQ=255)

                              gAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTaaaa                                                                                                       <  1:838523/140‑1 (MQ=255)

                                aGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAaga                                                                                                     <  1:354816/140‑1 (MQ=255)

                                   tACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCt                                                                                                  <  2:69353/140‑1 (MQ=255)

                                    aCCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCtt                                                                                                 <  2:95897/140‑1 (MQ=255)

                                    aCCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCtt                                                                                                 <  2:724913/140‑1 (MQ=255)

                                    aCCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCtt                                                                                                 <  1:48147/140‑1 (MQ=255)

                                    aCCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCtt                                                                                                 >  1:496931/1‑140 (MQ=255)

                                         aaCCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGTAAACTACTAAAAGAGCTTcatac                                                                                            >  1:230293/1‑140 (MQ=255)

                                         aaCCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAact                                                                                                              >  1:434763/1‑122 (MQ=255)

                                         aaCCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAact                                                                                                              <  2:434763/122‑1 (MQ=255)

                                              cTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAAt                                                                                       >  2:478957/1‑140 (MQ=255)

                                                   aCCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAAcc                                                                                  >  2:280044/1‑140 (MQ=255)

                                                        aCAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTc                                                                             >  2:916809/1‑140 (MQ=255)

                                                                         ccTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCcgcg                                                            <  2:496931/140‑1 (MQ=255)

                                                                         ccTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCcgcg                                                            >  2:700883/1‑140 (MQ=255)

                                                                         ccTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCATCATACATAATCAACCTGTTCCTTGCGGAGTTGCcgcg                                                            <  1:745459/140‑1 (MQ=255)

                                                                                   aggaggCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCt                                                  <  2:753875/140‑1 (MQ=255)

                                                                                   aggaggCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCt                                                  <  1:758323/140‑1 (MQ=255)

                                                                                     gaggCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTc                                                <  2:820578/140‑1 (MQ=255)

                                                                                          tCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCa                                           >  1:511649/1‑140 (MQ=255)

                                                                                            ccATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAAt                                         >  2:634996/1‑140 (MQ=255)

                                                                                              atatCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATaa                                       >  1:865463/1‑140 (MQ=255)

                                                                                                      gcCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAg                               >  2:189885/1‑140 (MQ=255)

                                                                                                      gcCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAg                               >  2:82281/1‑140 (MQ=255)

                                                                                                      gcCTTTACCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAg                               >  2:821751/1‑140 (MQ=255)

                                                                                                            aCCTTGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTa                         <  1:911054/140‑1 (MQ=255)

                                                                                                               ttGCTGCAGCATGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATc                      <  1:189885/140‑1 (MQ=255)

                                                                                                                     cagcaTGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTccc                >  1:78758/1‑140 (MQ=255)

                                                                                                                        caTGATCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACt             >  2:241543/1‑140 (MQ=255)

                                                                                                                            aTCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAg         >  1:290204/1‑140 (MQ=255)

                                                                                                                            aTCAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAg         <  2:250538/140‑1 (MQ=255)

                                                                                                                              cAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCg       <  2:677914/140‑1 (MQ=255)

                                                                                                                              cAGACCAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCg       <  1:916809/140‑1 (MQ=255)

                                                                                                                                  ccAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGgtg   >  2:544499/1‑140 (MQ=255)

                                                                                                                                  ccAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGgtg   >  2:36112/1‑140 (MQ=255)

                                                                                                                                  ccAACAGGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACAAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGgtg   >  1:920104/1‑140 (MQ=255)

                                                                                                                                   caacagGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGgtgt  <  1:26497/140‑1 (MQ=255)

                                                                                                                                   caacagGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGgtgt  <  2:865463/140‑1 (MQ=255)

CGTTGCCAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAAGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGT  >  NC_000913/3322323‑3322593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A