Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	128	54.7	1845674	99.3%	1832754	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,582,425	T→C	V188A (GTA→GCA)	yhhZ →	putative endonuclease YhhZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,582,425	0	T	C	100.0%	285.3 / NA	76	V188A (GTA→GCA)	yhhZ	putative endonuclease YhhZ
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (32/44); total (32/44)

AAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAG  >  NC_000913/3582288‑3582561

aaaTACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGcat                                                                                                                                        <  1:2233/140‑1 (MQ=255)

 aaTACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGcata                                                                                                                                       >  2:59943/1‑140 (MQ=255)

  aTACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATAc                                                                                                                                      >  2:761324/1‑140 (MQ=255)

        aGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATggg                                                                                                                                <  2:402193/140‑1 (MQ=255)

        aGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATggg                                                                                                                                <  1:122200/140‑1 (MQ=255)

         gTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGa                                                                                                                               <  1:834514/140‑1 (MQ=255)

          ttCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGaa                                                                                                                              >  1:63935/1‑140 (MQ=255)

            cAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACa                                                                                                                            >  1:582986/1‑140 (MQ=255)

             aGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGAGGCGCATACCATGGGAACAt                                                                                                                           >  1:164678/1‑140 (MQ=255)

               cTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTg                                                                                                                         >  1:100549/1‑140 (MQ=255)

               cTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTg                                                                                                                         >  2:178663/1‑140 (MQ=255)

               cTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTg                                                                                                                         <  1:910682/140‑1 (MQ=255)

                  tttGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAg                                                                                                                      <  2:854054/140‑1 (MQ=255)

                  tttGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAg                                                                                                                      <  2:63935/140‑1 (MQ=255)

                    tGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGa                                                                                                                    <  2:582986/140‑1 (MQ=255)

                    tGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGa                                                                                                                    >  2:565907/1‑140 (MQ=255)

                    tGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGa                                                                                                                    <  1:693174/140‑1 (MQ=255)

                    tGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGa                                                                                                                    >  2:858689/1‑140 (MQ=255)

                         cTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCag                                                                                                               >  2:830370/1‑140 (MQ=255)

                            ccTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAgaga                                                                                                            >  2:201538/1‑140 (MQ=255)

                            ccTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAgaga                                                                                                            >  2:649215/1‑140 (MQ=255)

                              tGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATa                                                                                                          >  2:823050/1‑140 (MQ=255)

                                    ttATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATAc                                                                                                         >  2:210779/1‑135 (MQ=255)

                                    ttATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATAc                                                                                                         <  1:210779/135‑1 (MQ=255)

                                    ttATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCgg                                                                                                    >  1:103004/1‑140 (MQ=255)

                                      ataACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGaa                                                                                                  >  1:381288/1‑140 (MQ=255)

                                      ataACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGaa                                                                                                  >  1:192404/1‑140 (MQ=255)

                                         aCCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGaaaaa                                                                                               <  2:189472/140‑1 (MQ=255)

                                               tgtttTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGt                                                                                         >  2:127186/1‑140 (MQ=255)

                                               tgtttTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGt                                                                                         >  2:209459/1‑140 (MQ=255)

                                                  ttttACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGga                                                                                      <  2:686791/140‑1 (MQ=255)

                                                   tttACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGgag                                                                                     <  2:350655/140‑1 (MQ=255)

                                                    ttACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGgaga                                                                                    <  2:418735/140‑1 (MQ=255)

                                                    ttACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGgaga                                                                                    <  1:895246/140‑1 (MQ=255)

                                                      aCCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATg                                                                                  <  2:705129/140‑1 (MQ=255)

                                                            ttCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATaa                                                                            <  1:175894/140‑1 (MQ=255)

                                                             tCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATaaa                                                                           <  2:662511/140‑1 (MQ=255)

                                                                 aaaaCCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGc                                                                       >  2:819766/1‑140 (MQ=255)

                                                                   aaCCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTa                                                                     <  1:522380/140‑1 (MQ=255)

                                                                   aaCCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTa                                                                     <  2:381288/140‑1 (MQ=255)

                                                                   aaCCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTa                                                                     <  2:396713/140‑1 (MQ=255)

                                                                   aaCCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTa                                                                     <  1:277086/140‑1 (MQ=255)

                                                                   aaCCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTa                                                                     <  1:201538/140‑1 (MQ=255)

                                                                   aaCCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTa                                                                     <  1:850947/140‑1 (MQ=255)

                                                                   aaCCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTa                                                                     <  1:211776/140‑1 (MQ=255)

                                                                   aaCCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTa                                                                     <  1:59943/140‑1 (MQ=255)

                                                                         acaaaTAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCa                                                               >  2:557910/1‑140 (MQ=255)

                                                                          caaaTAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCaa                                                              <  1:761324/140‑1 (MQ=255)

                                                                                atCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTa                                                        <  1:178663/140‑1 (MQ=255)

                                                                                atCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTa                                                        <  1:504159/140‑1 (MQ=255)

                                                                                    tttCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATg                                                    >  2:816807/1‑140 (MQ=255)

                                                                                     ttCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGa                                                   >  2:513386/1‑140 (MQ=255)

                                                                                        aaaaCGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGaaaa                                                <  1:71523/140‑1 (MQ=255)

                                                                                            cGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACa                                            <  1:209459/140‑1 (MQ=255)

                                                                                            cGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACa                                            <  2:816799/140‑1 (MQ=255)

                                                                                             gTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAg                                           <  2:898536/140‑1 (MQ=255)

                                                                                               tAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCg                                         >  2:115012/1‑140 (MQ=255)

                                                                                               tAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCg                                         >  1:24512/1‑140 (MQ=255)

                                                                                               tAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCg                                         >  1:475505/1‑140 (MQ=255)

                                                                                               tAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCg                                         <  2:103004/140‑1 (MQ=255)

                                                                                                   cAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCa                                     <  2:405458/140‑1 (MQ=255)

                                                                                                   cAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCa                                     <  2:504276/140‑1 (MQ=255)

                                                                                                       aaaGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGc                                 <  2:744179/140‑1 (MQ=255)

                                                                                                       aaaGCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGc                                 >  1:749125/1‑140 (MQ=255)

                                                                                                          gCTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGAt                              <  1:193690/140‑1 (MQ=255)

                                                                                                           cTATTGGCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATa                             >  2:30101/1‑140 (MQ=255)

                                                                                                                ggCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAAc                        <  1:823050/140‑1 (MQ=255)

                                                                                                                ggCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAAc                        <  1:72800/140‑1 (MQ=255)

                                                                                                                 gCAGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACa                       <  1:802003/140‑1 (MQ=255)

                                                                                                                   aGGCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGc                     <  1:115012/140‑1 (MQ=255)

                                                                                                                     gCTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCAt                   >  2:320417/1‑140 (MQ=255)

                                                                                                                      cTACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATc                  >  1:11605/1‑140 (MQ=255)

                                                                                                                       tACTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCt                 <  1:557910/140‑1 (MQ=255)

                                                                                                                        aCTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTa                >  1:5270/1‑140 (MQ=255)

                                                                                                                        aCTTGCTGCTGGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTa                >  1:511834/1‑140 (MQ=255)

                                                                                                                               gctgGTGGCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCtttt         >  2:230512/1‑140 (MQ=255)

                                                                                                                                   gtggCGCATTCAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACa     <  2:94969/140‑1 (MQ=255)

                                                                                                                                   gtggCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACa     >  2:111221/1‑140 (MQ=255)

                                                                                                                                   gtggCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACa     <  2:24512/140‑1 (MQ=255)

                                                                                                                                     ggCGCATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACaaa   <  2:629678/140‑1 (MQ=255)

                                                                                                                                      gcgcATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAg  <  1:649215/140‑1 (MQ=255)

                                                                                                                                      gcgcATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAg  <  2:11605/140‑1 (MQ=255)

AAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAG  >  NC_000913/3582288‑3582561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A