Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F28 I1 R1
|
224 |
87.5 |
2960670 |
99.3% |
2939945 |
138.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,591,352 |
G→A |
A325V (GCG→GTG) |
ugpB ← |
sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,591,352 | 0 | G | A | 100.0%
| 144.2
/ NA
| 45 | A325V (GCG→GTG) | ugpB | sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/29); total (16/29) |
TATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTAAGGCATCATGCCTACGC > NC_000913/3591215‑3591469
|
tATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGC‑TTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACca < 2:1407289/140‑1 (MQ=255)
aTCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACca < 1:1240403/140‑1 (MQ=255)
aTCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACca < 2:454304/140‑1 (MQ=255)
aTCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCG‑TTTCTGGCTTCGCGAGGAAATCGAGGAACTTcaccac < 2:1449387/140‑1 (MQ=255)
tCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTcaccac > 1:1253644/1‑140 (MQ=255)
tCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGATTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTcaccac > 1:306132/1‑140 (MQ=255)
cGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCacac < 2:14006/140‑1 (MQ=255)
ccccTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCg < 1:4060/140‑1 (MQ=255)
cccTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACcaca < 2:157223/136‑1 (MQ=255)
cccTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACcaca > 1:157223/1‑136 (MQ=255)
cccTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCgg > 1:702145/1‑140 (MQ=255)
ccTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGt > 1:1187173/1‑140 (MQ=255)
tGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACcaca > 2:81660/1‑133 (MQ=255)
tGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACcaca < 1:81660/133‑1 (MQ=255)
tGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGtat > 1:851725/1‑140 (MQ=255)
ttCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTtc > 1:241751/1‑140 (MQ=255)
ctcATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTtctt < 2:689270/140‑1 (MQ=255)
ctcATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTtctt < 2:540992/140‑1 (MQ=255)
aaaaGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTtctttatct < 2:103724/140‑1 (MQ=255)
cccTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTAcc < 2:1181724/140‑1 (MQ=255)
cTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCt < 2:654199/140‑1 (MQ=255)
tGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTg > 1:338733/1‑140 (MQ=255)
cTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCa < 2:128381/140‑1 (MQ=255)
cTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCa > 2:153099/1‑140 (MQ=255)
tCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCAt < 2:375503/140‑1 (MQ=255)
gggTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCAcc < 2:851725/140‑1 (MQ=255)
gtcaggtcaTACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCca < 1:488665/140‑1 (MQ=255)
aggtcaTACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGCAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCa < 2:1469044/130‑1 (MQ=255)
aggtcaTACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTAcc > 1:313445/1‑125 (MQ=255)
aggtcaTACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTAcc < 2:313445/125‑1 (MQ=255)
aggtcaTACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCa > 1:1469044/1‑130 (MQ=255)
gctgctTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACCCCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTcc < 2:80577/140‑1 (MQ=255)
gctTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTccgcc < 2:1420370/140‑1 (MQ=255)
gctTCGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTccgcc > 1:262321/1‑140 (MQ=255)
ctTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTccgccg > 2:92183/1‑140 (MQ=255)
ctTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTccgccg > 1:494195/1‑140 (MQ=255)
gATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATgg < 1:1368841/140‑1 (MQ=255)
gATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTtgt > 1:1303473/1‑140 (MQ=255)
aTGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCAt < 2:1303473/140‑1 (MQ=255)
ccACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCgg < 2:262321/140‑1 (MQ=255)
cACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACC‑GTATACGTTTCTTTATCTTTCCCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGcg < 2:315015/140‑1 (MQ=255)
cggCGTTTTCTGGTTTCTAGAGGAAACCGGGTACCTTCGCCACGCCGGTATACGTTTATTTCTCTTAACCCTTCCACGCCCACGGTCTGGATCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTaa < 2:306132/138‑1 (MQ=255)
cAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTaa < 2:1253644/140‑1 (MQ=255)
aGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTAAg < 1:153099/140‑1 (MQ=255)
cGTTTTCTGGCTTCGCGAGGAAATCGAGGACCTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTaa < 2:1249882/137‑1 (MQ=255)
cGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTaa > 1:1249882/1‑137 (MQ=255)
ttttCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTCCCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTAAGGcat < 2:186665/140‑1 (MQ=255)
tttCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGc > 1:682665/1‑127 (MQ=255)
tttCTGGCTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGc < 2:682665/127‑1 (MQ=255)
cTTCGCGAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTAAGGCATCATGCCTa > 1:525653/1‑140 (MQ=255)
cgcgAGGAAATCGAGGAACTTCACCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTAAGGCATCATGCCTACGc < 1:388970/140‑1 (MQ=255)
|
TATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCCACACCGGTATACGTTTCTTTATCTTTACCCTGCATCACCCACAGGCTGGCTCCGCCGATAATGGCGTTTTGTGGCGCATCTTTCGCATCGGCGTCGTAAGGCATCATGCCTACGC > NC_000913/3591215‑3591469
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
