breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 310 (T)9→10 intergenic (+55/‑27) thrL → / → thrA thr operon leader peptide/fused aspartate kinase/homoserine dehydrogenase 1
RA 746 T→C V137A (GTA→GCA)  thrA → fused aspartate kinase/homoserine dehydrogenase 1
RA 30,689 G→A A347T (GCA→ACA)  carA → carbamoyl phosphate synthetase subunit alpha
RA 62,358 C→T A303T (GCA→ACA)  rapA ← RNA polymerase‑binding ATPase and RNAP recycling factor
RA 65,698 T→C D28G (GAC→GGC)  polB ← DNA polymerase II
RA 72,644 C→T G95E (GGG→GAG)  thiQ ← thiamine ABC transporter ATP binding subunit
RA 75,607 A→G intergenic (‑127/+37) thiB ← / ← sgrR thiamine ABC transporter periplasmic binding protein/DNA‑binding transcriptional dual regulator SgrR
RA 84,655 A→G V96V (GTA→GTG leuO → DNA‑binding transcriptional dual regulator LeuO
RA 88,279 A→G E84E (GAA→GAG cra → DNA‑binding transcriptional dual regulator Cra
RA 100,328 G→A A229T (GCG→ACG)  murG → N‑acetylglucosaminyl transferase
RA 115,813 A→G G429G (GGT→GGC hofB ← T2SSE family protein
RA 122,534 A→G N148S (AAC→AGC)  pdhR → DNA‑binding transcriptional dual regulator PdhR
RA 128,530 T→C F207L (TTC→CTC)  lpd → lipoamide dehydrogenase
RA 134,603 G→A V72V (GTG→GTA yacL → UPF0231 family protein YacL
RA 155,212 T→C D72G (GAC→GGC)  htrE ← putative fimbrial usher protein HtrE
RA 177,927 A→G V179A (GTA→GCA)  btuF ← vitamin B12 ABC transporter periplasmic binding protein
RA 234,901 T→C L29P (CTG→CCG)  yafS → putative S‑adenosyl‑L‑methionine‑dependent methyltransferase
RA 255,174 (C)6→7 coding (543/1458 nt) pepD ← peptidase D
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 273,814 T→C I323T (ATC→ACC)  ykfC → CP4‑6 prophage; PF00078 domain‑containing protein YkfC
RA 298,429 A→G Y100H (TAT→CAT)  paoD ← molybdenum cofactor insertion chaperone for PaoABC
RA 298,818 A→G V706A (GTG→GCG)  paoC ← aldehyde dehydrogenase: molybdenum cofactor‑binding subunit
RA 308,054 (C)7→8 coding (1279/2526 nt) ecpC ← putative fimbrial usher protein EcpC
RA 339,529 A→G N402S (AAC→AGC)  yahG → DUF1116 domain‑containing protein YahG
RA 365,156 T→C N384D (AAC→GAC)  lacZ ← beta‑galactosidase
RA 380,022 (G)10→11 intergenic (‑141/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 384,281 A→G S112S (TCT→TCC yaiS ← putative deacetylase YaiS
RA 394,042 A→G pseudogene (1073/1449 nt) yaiT → putative autotransporter YaiT
RA 396,114 A→G G58G (GGT→GGC ampH ← peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase
RA 401,670 T→C intergenic (+24/‑77) iraP → / → phoA anti‑adaptor protein for sigma(S) stabilization/alkaline phosphatase
RA 476,542 A→G noncoding (95/114 nt) ffs → signal recognition particle 4.5S RNA
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 529,055 (T)8→9 pseudogene (416/491 nt) ybbD → putative uncharacterized protein YbbD
RA 595,224 G→A P74S (CCG→TCG)  cusR ← DNA‑binding transcriptional activator CusR
RA 669,398 Δ1 bp coding (510/612 nt) cobC ← putative adenosylcobalamin phosphatase/alpha‑ribazole phosphatase
RA 717,125 (G)6→7 coding (1141/1320 nt) potE ← putrescine:H(+) symporter/putrescine:ornithine antiporter PotE
RA 732,401 A→G D940G (GAC→GGC)  rhsC → rhs element protein RhsC
RA 733,643 T→C F1354S (TTC→TCC)  rhsC → rhs element protein RhsC
RA 770,985 (T)5→4 intergenic (+374/‑473) mngB → / → cydA alpha‑mannosidase/cytochrome bd‑I ubiquinol oxidase subunit I
RA 789,580 C→T E134K (GAA→AAA)  galK ← galactokinase
RA 797,241 C→T Q230* (CAA→TAA)  modC → molybdate ABC transporter ATP binding subunit
RA 812,985 T→C intergenic (+38/‑541) bioD → / → uvrB dethiobiotin synthetase/excision nuclease subunit B
RA 852,439 T→C L53L (CTA→CTG opgE ← phosphoethanolamine transferase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 899,900 C→T A226T (GCA→ACA)  artJ ← L‑arginine ABC transporter periplasmic binding protein
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 950,098 (A)7→8 coding (431/591 nt) ycaK → putative NAD(P)H‑dependent oxidoreductase YcaK
RA 989,615 A→G Y248H (TAT→CAT)  pncB ← nicotinate phosphoribosyltransferase
RA 999,141 A→G T218A (ACC→GCC)  elfD → putative fimbrial chaperone ElfD
RA 1,002,224 (G)7→6 coding (418/1071 nt) elfG → putative fimbrial‑like adhesin protein
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,052,491 G→A A291V (GCC→GTC)  yccM ← putative 4Fe‑4S membrane protein
RA 1,080,256 G→A A318T (GCG→ACG)  putP → proline:Na(+) symporter
RA 1,110,873 C→T T4I (ACA→ATA)  opgH → osmoregulated periplasmic glucans (OPGs) biosynthesis protein H
RA 1,126,649 C→T H165Y (CAT→TAT)  yceH → DUF480 domain‑containing protein YceH
RA 1,131,872 A→G T7A (ACC→GCC)  flgD → flagellar biosynthesis, initiation of hook assembly
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,154,503 Δ1 bp coding (392/1023 nt) mltG → endolytic murein transglycosylase
RA 1,164,550 T→C G152G (GGT→GGC nagZ → beta‑N‑acetylhexosaminidase
RA 1,172,172 G→A P598L (CCG→CTG)  mfd ← transcription‑repair coupling factor
RA 1,172,514 A→G V484A (GTC→GCC)  mfd ← transcription‑repair coupling factor
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,243,627 Δ1 bp coding (448/612 nt) emtA → lytic murein transglycosylase E
RA 1,263,632 (G)5→4 intergenic (‑132/‑82) lolB ← / → hemA outer membrane lipoprotein LolB/glutamyl‑tRNA reductase
RA 1,266,530 T→C V146A (GTC→GCC)  prmC → protein‑(glutamine‑N(5)) methyltransferase
RA 1,270,545 G→T intergenic (+85/+204) rdlC → / ← chaA small regulatory antisense RNA RdlC/Na(+)/K(+):H(+) antiporter ChaA
RA 1,272,125 T→C Y3H (TAT→CAT)  chaB → putative cation transport regulator ChaB
RA 1,277,410 T→C D70G (GAC→GGC)  narX ← sensory histidine kinase NarX
RA 1,298,959 T→C V121A (GTG→GCG)  ychE → putative inner membrane protein
RA 1,310,751 T→C T39A (ACA→GCA)  yciI ← protein YciI
RA 1,334,153 C→T T100M (ACG→ATG)  cysB → DNA‑binding transcriptional dual regulator CysB
RA 1,357,342 A→G intergenic (‑232/+81) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,365,094 A→G Y288C (TAC→TGC)  puuB → gamma‑glutamylputrescine oxidase
RA 1,413,413 A→G Y14H (TAC→CAC)  xisR ← Rac prophage; excisionase
RA 1,472,368 A→G pseudogene (1850/3495 nt) ydbA → putative outer membrane protein, N‑terminal fragment
RA 1,475,398 T→C S85S (AGT→AGC ydbD → DUF2773 domain‑containing protein YdbD
RA 1,510,788 (A)8→9 coding (786/1407 nt) ydcR → fused putative DNA‑binding transcriptional regulator/putative aminotransferase YdcR
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,541,861 A→G W244R (TGG→CGG)  narZ ← nitrate reductase Z subunit alpha
RA 1,568,308 A→G G172G (GGT→GGC yddW ← putative lipoprotein YddW
RA 1,595,543 A→G pseudogene (2544/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,603,810 T→C G264G (GGT→GGC lsrC → Autoinducer‑2 ABC transporter membrane subunit LsrC
RA 1,617,459 C→T T144T (ACC→ACT ydeA → L‑arabinose exporter
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,648,247 A→G D105G (GAT→GGT)  dicA → DNA‑binding transcriptional dual regulator DicA
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,712,712 (T)6→5 intergenic (+554/‑57) nth → / → dtpA endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
RA 1,724,478 (G)6→7 coding (178/522 nt) sodC ← superoxide dismutase (Cu‑Zn)
RA 1,761,725 T→C T23A (ACA→GCA)  sufD ← Fe‑S cluster scaffold complex subunit SufD
RA 1,768,441 (G)6→5 coding (245/3057 nt) ydiJ ← putative FAD‑linked oxidoreductase
RA 1,771,123 T→C V18A (GTG→GCG)  ydiM → putative exporter YdiM
RA 1,799,071 T→A intergenic (‑129/+155) pheS ← / ← pheM phenylalanine‑‑tRNA ligase subunit alpha/pheST‑ihfA operon leader peptide
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,886,585 G→A V247V (GTG→GTA yeaX → carnitine monooxygenase subunit YeaX
RA 1,919,648 T→C T713T (ACT→ACC yebT → intermembrane transport protein YebT
RA 1,920,784 T→C F188L (TTC→CTC)  rsmF → 16S rRNA m(5)C1407 methyltransferase
RA 1,925,000 (T)6→7 intergenic (‑31/‑108) yobA ← / → holE CopC domain‑containing protein/DNA polymerase III subunit theta
RA 1,929,001 T→C intergenic (‑162/+47) ptrB ← / ← yebE oligopeptidase B/conserved inner membrane protein YebE
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,985,515 G→A A205V (GCA→GTA)  araF ← arabinose ABC transporter periplasmic binding protein
RA 2,057,676 (C)7→8 intergenic (+102/+351) yeeN → / ← asnW putative transcriptional regulator YeeN/tRNA‑Asn
RA 2,096,209 G→A V122V (GTG→GTA hisF → imidazole glycerol phosphate synthase subunit HisF
RA 2,113,672 G→A A219V (GCC→GTC)  galF ← UTP:glucose‑1‑phosphate uridylyltransferase, low activity
RA 2,114,380 (T)7→6 intergenic (‑53/+122) galF ← / ← wcaM UTP:glucose‑1‑phosphate uridylyltransferase, low activity/putative colanic acid biosynthesis protein WcaM
RA 2,124,416 T→C K35K (AAA→AAG cpsB ← mannose‑1‑phosphate guanylyltransferase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,200,454 (A)8→7 coding (176/3633 nt) yehI → DUF4132 domain‑containing protein YehI
RA 2,202,995 G→A S906N (AGC→AAC)  yehI → DUF4132 domain‑containing protein YehI
RA 2,228,774 T→C intergenic (‑16/‑208) mdtQ ← / → yohP putative multidrug resistance outer membrane protein MdtQ/putative membrane protein YohP
RA 2,304,077 T→C V58A (GTA→GCA)  eco → serine protease inhibitor ecotin
RA 2,309,596 Δ1 bp coding (810/1065 nt) ada ← DNA‑binding transcriptional dual regulator/DNA repair protein Ada
RA 2,313,965 Δ1 bp coding (478/2673 nt) rcsD → RcsD phosphotransferase
RA 2,328,828 C→T E324K (GAA→AAA)  yfaQ ← tandem DUF2300 domain‑containing protein YfaQ
RA 2,339,206 T→C D72G (GAC→GGC)  gyrA ← DNA gyrase subunit A
RA 2,352,253 T→C Y41C (TAC→TGC)  glpT ← sn‑glycerol 3‑phosphate:phosphate antiporter
RA 2,394,188 Δ1 bp coding (859/1842 nt) nuoL ← NADH:quinone oxidoreductase subunit L
RA 2,396,354 A→G Y33H (TAC→CAC)  nuoI ← NADH:quinone oxidoreductase subunit I
RA 2,407,333 (A)7→6 intergenic (‑692/‑228) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
RA 2,412,143 T→C Y151C (TAC→TGC)  yfbU ← UPF0304 family protein YfbU
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,453,508 G→A pseudogene (1494/2646 nt) yfcU ← putative outer membrane usher protein YfcU
JC 2,462,889 (C)9→12 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,539,741 T→C E358E (GAA→GAG cysA ← sulfate/thiosulfate ABC transporter ATP binding subunit
RA 2,572,965 T→C I181V (ATC→GTC)  eutD ← phosphate acetyltransferase EutD
RA 2,602,017 (C)7→8 coding (200/2019 nt) hyfB → hydrogenase 4 component B
RA 2,673,991 A→G F233S (TTC→TCC)  yphB ← putative aldose 1‑epimerase YphB
RA 2,678,695 T→C M225V (ATG→GTG)  yphF ← putative ABC transporter periplasmic binding protein YphF
RA 2,705,998 G→A T376I (ACC→ATC)  lepA ← elongation factor 4
RA 2,716,526 G→A intergenic (‑77/‑228) grcA ← / → ung stress‑induced alternate pyruvate formate‑lyase subunit/uracil‑DNA glycosylase
RA 2,718,698 T→C intergenic (‑170/‑37) yfiF ← / → trxC putative methyltransferase/reduced thioredoxin 2
RA 2,719,486 T→C R88R (CGT→CGC yfiP → conserved protein YfiP
RA 2,741,073 +A coding (78/1071 nt) aroF ← 3‑deoxy‑7‑phosphoheptulonate synthase, Tyr‑sensitive
RA 2,766,360 A→G D148G (GAC→GGC)  rnlA → CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system
RA 2,767,445 (C)7→8 intergenic (+90/‑265) rnlB → / → yfjP CP4‑57 prophage; antitoxin RnlB/CP4‑57 prophage; putative GTP‑binding protein YfjP
RA 2,773,113 C→T pseudogene (70/1016 nt) yfjV ← CP4‑57 prophage; putative arsenite transporter, N‑terminal fragment
RA 2,778,930 (C)6→5 coding (3797/4581 nt) ypjA ← adhesin‑like autotransporter YpjA
RA 2,802,472 T→C A375A (GCT→GCC nrdE → ribonucleoside‑diphosphate reductase 2 subunit alpha
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,824,038 A→G S103S (AGT→AGC pncC ← NMN aminohydrolase
RA 2,844,820 A→G V551A (GTG→GCG)  hycE ← formate hydrogenlyase subunit HycE
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,867,956 G→A G266G (GGC→GGT nlpD ← murein hydrolase activator NlpD
RA 2,880,579 (A)7→8 coding (470/675 nt) casD ← type I‑E CRISPR system Cascade subunit CasD
RA 2,880,898 T→C T51A (ACT→GCT)  casD ← type I‑E CRISPR system Cascade subunit CasD
RA 2,910,723 A→G intergenic (‑38/+33) mazG ← / ← mazF nucleoside triphosphate pyrophosphohydrolase/endoribonuclease toxin MazF
RA 2,925,012 T→C H90R (CAC→CGC)  syd ← SecY‑interacting protein
RA 2,940,984 (G)5→6 coding (260/1101 nt) rlmM ← 23S rRNA 2'‑O‑ribose C2498 methyltransferase
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,952,692 T→C A1104A (GCA→GCG recB ← exodeoxyribonuclease V subunit RecB
RA 2,980,296 +C coding (340/693 nt) ygeA ← amino acid racemase
RA 2,981,901 (C)5→6 coding (282/1419 nt) araE ← arabinose:H(+) symporter
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,158,773 A→G A49A (GCA→GCG yqhH → lipoprotein YqhH
RA 3,188,538 C→T pseudogene (1107/2434 nt) yqiG → putative outer membrane usher protein YqiG
RA 3,190,744 A→G Y38C (TAC→TGC)  yqiI → putative fimbrial protein YqiI
RA 3,207,828 G→A W153* (TGG→TAG)  ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,237,712 G→A Q134Q (CAG→CAA ygjR → putative oxidoreductase YgjR
RA 3,244,741 T→C M1M (ATG→GTG) † uxaC ← D‑glucoronate/D‑galacturonate isomerase
RA 3,250,172 G→A W32* (TGG→TGA yqjK → conserved protein YqjK
RA 3,254,967 A→G T217A (ACG→GCG)  yhaK → bicupin‑related protein
RA 3,268,304 (C)6→7 coding (451/540 nt) yhaB → protein YhaB
RA 3,300,238 T→C S91S (AGT→AGC yhbQ → DNA damage response nuclease YhbQ
RA 3,318,389 T→C intergenic (‑100/‑248) metY ← / → argG tRNA‑Met/argininosuccinate synthetase
RA 3,332,680 (C)6→7 coding (56/966 nt) yhbE ← inner membrane protein YhbE
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,371,435 T→C G380G (GGA→GGG nanT ← N‑acetylneuraminate:H(+) symporter
RA 3,374,484 G→A G2G (GGC→GGT nanR ← DNA‑binding transcriptional dual regulator NanR
RA 3,375,991 T→C F375L (TTT→CTT)  dcuD → putative transporter DcuD
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,411,037 C→T A253V (GCA→GTA)  dusB → tRNA‑dihydrouridine synthase B
RA 3,433,448 A→G V38A (GTA→GCA)  smf ← protein Smf
RA 3,443,769 T→C E110G (GAG→GGG)  secY ← Sec translocon subunit SecY
RA 3,451,262 C→T E35K (GAA→AAA)  rplB ← 50S ribosomal subunit protein L2
RA 3,456,803 (C)6→7 coding (427/1953 nt) gspD → Type II secretion system protein GspD
RA 3,495,653 T→C L548P (CTG→CCG)  nirB → nitrite reductase catalytic subunit NirB
RA 3,501,834 T→C H189H (CAT→CAC frlB → fructoselysine 6‑phosphate deglycase
RA 3,540,380 T→C V73A (GTT→GCT)  feoA → ferrous iron transport protein A
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,565,382 A→G Y400Y (TAT→TAC glgP ← glycogen phosphorylase
RA 3,591,352 G→A A325V (GCG→GTG)  ugpB ← sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein
RA 3,603,835 T→C I137V (ATT→GTT)  ftsY ← signal recognition particle receptor
RA 3,629,470 (T)5→6 coding (69/2736 nt) rbbA ← ribosome‑associated ATPase
RA 3,637,817 T→C V59A (GTA→GCA)  pitA → metal phosphate:H(+) symporter PitA
RA 3,649,771 (C)5→6 coding (837/1290 nt) arsB → arsenite/antimonite:H(+) antiporter
RA 3,728,118 (C)8→9 coding (1254/1455 nt) xylB ← xylulokinase
RA 3,786,297 T→C G346G (GGT→GGC gpmM → 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
RA 3,808,983 G→A L148L (CTG→CTA waaA → KDO transferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,838,343 C→T S32S (AGC→AGT yicL → putative inner membrane protein
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,940,954 A→G V125A (GTT→GCT)  yieP ← putative transcriptional regulator YieP
RA 4,011,402 T→C R109R (CGT→CGC bioP → biotin transporter
RA 4,018,973 A→G N40S (AAT→AGT)  ubiE → bifunctional 2‑octaprenyl‑6‑methoxy‑1,4‑benzoquinone methylase and S‑adenosylmethionine:2‑DMK methyltransferase
RA 4,023,265 T→C L177P (CTG→CCG)  tatC → twin arginine protein translocation system ‑ TatC protein
RA 4,138,433 T→C A201A (GCA→GCG gldA ← L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
RA 4,181,024 A→G intergenic (+99/‑221) rplL → / → rpoB 50S ribosomal subunit protein L12/RNA polymerase subunit beta
RA 4,200,096 A→G intergenic (+2/‑185) yjaG → / → hupA conserved protein YjaG/DNA‑binding protein HU‑alpha
RA 4,202,187 C→T P88S (CCG→TCG)  zraS → sensory histidine kinase ZraS
RA 4,206,742 T→C N264S (AAC→AGC)  purH ← bifunctional AICAR transformylase/IMP cyclohydrolase
RA 4,234,871 A→G T372A (ACT→GCT)  pgi → glucose‑6‑phosphate isomerase
RA 4,237,358 C→T S154S (AGC→AGT yjbG → capsule biosynthesis GfcC family protein YjbG
RA 4,276,165 A→G Y232C (TAT→TGT)  pdeC → c‑di‑GMP phosphodiesterase PdeC
RA 4,286,716 A→G V219A (GTA→GCA)  acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,307,299 (G)5→6 coding (501/930 nt) alsK ← D‑allose kinase
RA 4,339,091 T→C K477K (AAA→AAG adiA ← arginine decarboxylase, degradative
RA 4,344,573 A→G Q402R (CAG→CGG)  melB → melibiose:H(+)/Na(+)/Li(+) symporter
RA 4,395,669 A→G T29A (ACC→GCC)  tsaE → N(6)‑L‑threonylcarbamoyladenine synthase, TsaE subunit
RA 4,422,914 A→G E23E (GAA→GAG ulaE → L‑ribulose‑5‑phosphate 3‑epimerase UlaE
RA 4,437,443 A→G Y230C (TAC→TGC)  cysQ → 3'(2'),5'‑bisphosphate nucleotidase
RA 4,441,374 A→G intergenic (‑159/+164) ytfL ← / ← msrA putative inner membrane protein/methionine sulfoxide reductase A
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,452,352 G→A G432D (GGC→GAC)  ytfR → galactofuranose ABC transporter putative ATP binding subunit
RA 4,459,316 (A)7→8 intergenic (+5/+584) pmbA → / ← nrdG metalloprotease PmbA/anaerobic ribonucleoside‑triphosphate reductase activating protein
RA 4,465,733 A→G Y150H (TAC→CAC)  treB ← trehalose‑specific PTS enzyme IIBC component
RA 4,518,648 T→C L41P (CTA→CCA)  insA‑7 → KpLE2 phage‑like element; IS1 repressor protein InsA
RA 4,546,393 C→T A433V (GCA→GTA)  fimD → type I fimbriae usher protein
RA 4,592,474 G→A G273E (GGG→GAG)  tsr → methyl‑accepting chemotaxis protein Tsr
RA 4,600,250 A→G G242G (GGT→GGC dnaC ← DNA replication protein DnaC
RA 4,606,094 (G)8→9 noncoding (72/87 nt) leuV ← tRNA‑Leu
RA 4,624,649 T→C E47E (GAA→GAG ytjB ← protein Smp
RA 4,627,471 C→T R53C (CGT→TGT)  nadR → DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 731797–731807 732217–731990 184–421 28 [19] [23] 29 rhsC rhs element protein RhsC
* * ÷ NC_000913 1270048–1270094 1270524–1270504 411–477 25 [24] [24] 28 ldrC–rdlC ldrC,rdlC
* * ÷ NC_000913 3423770–3424234 3424525–3424238 5–756 26 [20] [22] 25 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3762345–3764072 3765191–3764077 6–2847 25 [24] [23] 25 rhsA rhs element protein RhsA
* * ÷ NC_000913 3942147–3943393 3946842–3946693 3301–4696 25 [23] [23] 25 [rrsC]–rrfC [rrsC],gltU,rrlC,rrfC

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =21 (0.240)127 (1.650) 66/242 0.0 90.2% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 9 (0.120)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780521 (0.240)67 (0.870) 43/242 0.5 83.0% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 9 (0.120)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)102 (1.210) 59/266 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =109 (1.250)114 (1.320) 66/272 0.1 67.6% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.010)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)83 (0.960) 55/274 0.3 94.3% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.060)coding (3907/4134 nt) rhsA rhs element protein RhsA