Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	128	54.7	1845674	99.3%	1832754	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,784,538	G→A	intergenic (‑96/+46)	grxC ← / ← yibN	glutaredoxin 3/putative sulfurtransferase YibN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,784,538	0	G	A	100.0%	187.0 / NA	53	intergenic (‑96/+46)	grxC/yibN	glutaredoxin 3/putative sulfurtransferase YibN
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/25); total (28/25)

GGCAGGTTTCTTTGGTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGGCCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTC  >  NC_000913/3784406‑3784671

ggCAGGTTTCTTTGGTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAAtt                                                                                                                                >  2:726066/1‑140 (MQ=255)

 gCAGGTTTCTTTGGTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTg                                                                                                                               >  2:875856/1‑140 (MQ=255)

 gCAGGTTTCTTTGGTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTg                                                                                                                               <  1:495410/140‑1 (MQ=255)

  cAGGTTTCTTTGGTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGc                                                                                                                              >  1:553993/1‑140 (MQ=255)

       ttCTTTGGTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTa                                                                                                                         <  2:193853/140‑1 (MQ=255)

            tGGTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGc                                                                                                                    <  1:875856/140‑1 (MQ=255)

             ggTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGcc                                                                                                                   <  1:476473/140‑1 (MQ=255)

              gTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCt                                                                                                                  <  2:656298/140‑1 (MQ=255)

              gTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCt                                                                                                                  <  1:860590/140‑1 (MQ=255)

                ataGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGc                                                                                                                >  2:355172/1‑140 (MQ=255)

                ataGATTTCAACATCGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGc                                                                                                                <  2:567256/140‑1 (MQ=255)

                 taGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGca                                                                                                               <  1:453176/140‑1 (MQ=255)

                    aTTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAACTG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGcatca                                                                                                            >  2:641507/1‑140 (MQ=255)

                    aTTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGcatca                                                                                                            >  1:529014/1‑140 (MQ=255)

                    aTTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGcatca                                                                                                            >  2:559864/1‑140 (MQ=255)

                         aaCATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGaaa                                                                                                       >  1:707400/1‑140 (MQ=255)

                            aTTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAgac                                                                                                    <  2:553993/140‑1 (MQ=255)

                            aTTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAgac                                                                                                    <  1:726066/140‑1 (MQ=255)

                              tGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATGGAGTGAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAg                                                                                                      >  1:826709/1‑140 (MQ=255)

                              tGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAgacga                                                                                                  <  1:559864/140‑1 (MQ=255)

                              tGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAgacga                                                                                                  >  1:672841/1‑140 (MQ=255)

                              tGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAgacga                                                                                                  >  1:75350/1‑140 (MQ=255)

                               ggCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAgacgac                                                                                                 <  2:672841/140‑1 (MQ=255)

                                gCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACa                                                                                                >  1:647013/1‑140 (MQ=255)

                                                tGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCcg                                                                                >  1:175201/1‑140 (MQ=255)

                                                tGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCcg                                                                                <  2:529014/140‑1 (MQ=255)

                                                 gTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCcgc                                                                               >  2:423916/1‑140 (MQ=255)

                                                            cTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGc                                                                    >  1:547773/1‑140 (MQ=255)

                                                            cTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGc                                                                    <  2:701588/140‑1 (MQ=255)

                                                                     cATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCg                                                           <  2:647013/140‑1 (MQ=255)

                                                                          tGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGccagc                                                      <  1:643624/140‑1 (MQ=255)

                                                                                     agcaAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGa                                           <  2:62744/140‑1 (MQ=255)

                                                                                     agcaAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGa                                           <  1:423916/140‑1 (MQ=255)

                                                                                                  gAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCcttctt                                  >  1:652563/1‑140 (MQ=255)

                                                                                                    gtgtCCCCAGAAGCTTCTAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTc                                <  2:826709/140‑1 (MQ=255)

                                                                                                    gtgtCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTc                                <  1:641507/140‑1 (MQ=255)

                                                                                                     tgtCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCa                               >  1:435583/1‑140 (MQ=255)

                                                                                                     tgtCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCa                               <  1:355172/140‑1 (MQ=255)

                                                                                                       tCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGa                                           >  1:910063/1‑126 (MQ=255)

                                                                                                       tCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGa                                           <  2:910063/126‑1 (MQ=255)

                                                                                                       tCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGc                             >  1:853173/1‑140 (MQ=255)

                                                                                                       tCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGc                             >  1:702382/1‑140 (MQ=255)

                                                                                                       tCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGc                             >  1:685721/1‑140 (MQ=255)

                                                                                                          ccAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGa                                           <  2:421388/123‑1 (MQ=255)

                                                                                                          ccAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGa                                           >  1:421388/1‑123 (MQ=255)

                                                                                                          ccAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACg                          <  1:583028/140‑1 (MQ=255)

                                                                                                             gAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAAt                       >  2:127906/1‑140 (MQ=255)

                                                                                                                 cTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACtt                   >  2:323780/1‑140 (MQ=255)

                                                                                                                 cTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACtt                   >  1:478287/1‑140 (MQ=255)

                                                                                                                   tataACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTgc                 >  2:8334/1‑140 (MQ=255)

                                                                                                                          aTAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAAc          <  2:707400/140‑1 (MQ=255)

                                                                                                                           tAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAAcc         >  1:240164/1‑140 (MQ=255)

                                                                                                                                  cTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTc  >  2:663170/1‑140 (MQ=255)

GGCAGGTTTCTTTGGTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGGCCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTC  >  NC_000913/3784406‑3784671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A