Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	128	54.7	1845674	99.3%	1832754	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,178,857	C→T	G137G (GGC→GGT)	rplK →	50S ribosomal subunit protein L11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,178,857	0	C	T	100.0%	169.9 / NA	48	G137G (GGC→GGT)	rplK	50S ribosomal subunit protein L11
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/25); total (23/25)

AACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAA  >  NC_000913/4178726‑4178989

aaCAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGt                                                                                                                              >  1:784740/1‑140 (MQ=255)

    aagacaaagTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTggag                                                                                                                          <  1:902018/140‑1 (MQ=255)

         aaagTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTa                                                                                                                     <  2:240770/140‑1 (MQ=255)

            gTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGTCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGa                                                                                                                  >  2:873537/1‑140 (MQ=255)

                gTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATg                                                                                                              >  2:886819/1‑140 (MQ=255)

                    aaTTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTa                                                                                                          <  1:479490/140‑1 (MQ=255)

                    aaTTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTa                                                                                                          >  1:780090/1‑140 (MQ=255)

                      tttCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTaaa                                                                                                        <  1:184800/140‑1 (MQ=255)

                            gcgcTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGTCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGAcc                                                                                                  <  1:873537/140‑1 (MQ=255)

                               cTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAg                                                                                               <  2:164215/140‑1 (MQ=255)

                               cTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAg                                                                                               <  1:71866/140‑1 (MQ=255)

                                     tGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATg                                                                                         <  1:886819/140‑1 (MQ=255)

                                              tCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTTACCAAGCGCATGCGTGTTATc                                                                                >  1:502520/1‑140 (MQ=255)

                                                           aGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATc                                                                                <  1:269478/127‑1 (MQ=255)

                                                           aGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATc                                                                                <  1:298522/127‑1 (MQ=255)

                                                           aGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATc                                                                                >  2:298522/1‑127 (MQ=255)

                                                           aGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATc                                                                                >  2:269478/1‑127 (MQ=255)

                                                            gCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTTCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGa                                                                  <  1:104429/140‑1 (MQ=255)

                                                            gCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGa                                                                  <  1:461966/140‑1 (MQ=255)

                                                                ccGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATg                                                                >  1:846392/1‑138 (MQ=255)

                                                                ccGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATg                                                                <  2:846392/138‑1 (MQ=255)

                                                                ccGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCa                                                              >  2:776718/1‑140 (MQ=255)

                                                                ccGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCa                                                              >  2:305566/1‑140 (MQ=255)

                                                                ccGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCACGAGAAAGTTGATGCa                                                              >  2:121286/1‑140 (MQ=255)

                                                                 cGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTTGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCaa                                                             <  2:202561/140‑1 (MQ=255)

                                                                      tGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCaaa                                                        <  2:784740/140‑1 (MQ=255)

                                                                      tGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCaaa                                                        <  1:126063/140‑1 (MQ=255)

                                                                           ggTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTa                                                   <  2:870441/140‑1 (MQ=255)

                                                                            gTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTAc                                                  <  1:790207/140‑1 (MQ=255)

                                                                               ccGACATTGAAGCGATTACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGAc                                               >  2:87892/1‑140 (MQ=255)

                                                                               ccGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGAc                                               >  1:340619/1‑140 (MQ=255)

                                                                               ccGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGAc                                               <  2:340619/140‑1 (MQ=255)

                                                                                       gAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGa                                       <  1:305566/140‑1 (MQ=255)

                                                                                             aTGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTAt                                 >  1:76183/1‑140 (MQ=255)

                                                                                              tGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATc                                >  1:129425/1‑140 (MQ=255)

                                                                                              tGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATc                                >  2:814042/1‑140 (MQ=255)

                                                                                                aCTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGc                              <  2:604727/140‑1 (MQ=255)

                                                                                                   cGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCAct                           <  1:87892/140‑1 (MQ=255)

                                                                                                     cTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCActgc                         >  2:492077/1‑140 (MQ=255)

                                                                                                     cTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCActgc                         >  2:741694/1‑140 (MQ=255)

                                                                                                            gAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAaga                  >  1:290851/1‑140 (MQ=255)

                                                                                                                  aCTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTTACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGc            <  2:502520/140‑1 (MQ=255)

                                                                                                                        cGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGc            <  2:667759/134‑1 (MQ=255)

                                                                                                                        cGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGc            >  1:667759/1‑134 (MQ=255)

                                                                                                                        cGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGc      >  2:614498/1‑140 (MQ=255)

                                                                                                                          ttCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTa    >  1:34981/1‑140 (MQ=255)

                                                                                                                            ccATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTaaa  >  2:340643/1‑140 (MQ=255)

                                                                                                                            ccATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTaaa  <  1:121286/140‑1 (MQ=255)

AACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAA  >  NC_000913/4178726‑4178989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A