Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 162 86.7 2935410 99.3% 2914862 138.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 614,801 A→G L215L (TTA→TTG)  entF → apo‑serine activating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913614,8010AG98.5% 222.0 / ‑4.7 66L215L (TTA→TTG) entFapo‑serine activating enzyme
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (31/34);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGTGTGCAGCGTACCGATTTAGCCCTTGCGCTGGCAGCCTTG  >  NC_000913/614674‑614930
                                                                                                                               |                                                                                                                                 
cTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCGCCTTTGCCGGGGCGCAgc                                                                                                                       <  2:243874/140‑1 (MQ=255)
cTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAgc                                                                                                                       <  2:815254/140‑1 (MQ=255)
   tCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGcc                                                                                                                    >  1:10708/1‑140 (MQ=255)
   tCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGcc                                                                                                                    >  1:53169/1‑140 (MQ=255)
   tCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGcc                                                                                                                    <  2:295674/140‑1 (MQ=255)
       tGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCgg                                                                                                                <  2:10708/140‑1 (MQ=255)
       tGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCgg                                                                                                                <  2:1082344/140‑1 (MQ=255)
       tGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCgg                                                                                                                <  2:251467/140‑1 (MQ=255)
          tGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAg                                                                                                             <  1:728768/140‑1 (MQ=255)
          tGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAg                                                                                                             >  2:728768/1‑140 (MQ=255)
              gTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGcc                                                                                                                    <  1:1080075/129‑1 (MQ=255)
              gTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGcc                                                                                                                    >  2:1080075/1‑129 (MQ=255)
                   agagTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTc                                                                                                                  >  1:962501/1‑126 (MQ=255)
                   agagTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTc                                                                                                                  <  2:962501/126‑1 (MQ=255)
                   agagTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTgc                                                                                                    >  2:199387/1‑140 (MQ=255)
                   agagTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTgc                                                                                                    >  1:521893/1‑140 (MQ=255)
                    gagTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTgcg                                                                                                   >  1:941788/1‑140 (MQ=255)
                      gTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTc                                                                                                                  >  1:1431197/1‑123 (MQ=255)
                      gTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTc                                                                                                                  <  2:1431197/123‑1 (MQ=255)
                      gTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTc                                                                                                                  >  1:586670/1‑123 (MQ=255)
                      gTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTc                                                                                                                  <  2:586670/123‑1 (MQ=255)
                      gTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGcc                                                                                                 >  2:663579/1‑140 (MQ=255)
                      gTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGcc                                                                                                 <  1:583912/140‑1 (MQ=255)
                      gTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGcc                                                                                                 >  2:1246179/1‑140 (MQ=255)
                         ccAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGa                                                                                              >  2:825324/1‑140 (MQ=255)
                             cAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACt                                                                                          >  1:982701/1‑140 (MQ=255)
                               aTACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTgg                                                                                        >  1:1159807/1‑140 (MQ=255)
                               aTACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTgg                                                                                        >  2:1371577/1‑140 (MQ=255)
                                tACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGa                                                                                       >  1:737281/1‑140 (MQ=255)
                                 aCCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGaa                                                                                      >  1:192221/1‑140 (MQ=255)
                                 aCCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGaa                                                                                      >  2:1411574/1‑140 (MQ=255)
                                        aaGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCg                                                                               <  2:982701/140‑1 (MQ=255)
                                            gAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACgg                                                                           <  1:825324/140‑1 (MQ=255)
                                             aaGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACggg                                                                          >  1:718264/1‑140 (MQ=255)
                                                     cAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCg                                                                  >  1:1382199/1‑140 (MQ=255)
                                                      aGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGc                                                                 <  1:199387/140‑1 (MQ=255)
                                                          tGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGc                                                             <  2:373294/140‑1 (MQ=255)
                                                          tGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGc                                                             <  2:1382199/140‑1 (MQ=255)
                                                           gATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCt                                                            <  1:1371577/140‑1 (MQ=255)
                                                           gATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCt                                                            <  1:1411574/140‑1 (MQ=255)
                                                                  cATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACg                                                     <  2:941788/140‑1 (MQ=255)
                                                                  cATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACg                                                     <  2:521893/140‑1 (MQ=255)
                                                                    ttCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCa                                                   <  1:276344/140‑1 (MQ=255)
                                                                         ggCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACttt                                              <  1:351954/140‑1 (MQ=255)
                                                                            aGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAg                                           <  1:464293/140‑1 (MQ=255)
                                                                              aaCAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGgt                                         >  2:136090/1‑140 (MQ=255)
                                                                                 aGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGtgtg                                      <  2:1159807/140‑1 (MQ=255)
                                                                                   cgtcgtCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACttt                                              <  1:1080501/130‑1 (MQ=255)
                                                                                   cgtcgtCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACttt                                              >  2:1080501/1‑130 (MQ=255)
                                                                                   cgtcgtCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGTGTGCa                                    >  2:851516/1‑140 (MQ=255)
                                                                                   cgtcgtCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGTGTGCa                                    <  1:203548/140‑1 (MQ=255)
                                                                                   cgtcgtCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGTGTGCa                                    >  2:571655/1‑140 (MQ=255)
                                                                                        tcAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGTGTGCAGCGTa                               >  1:1079650/1‑140 (MQ=255)
                                                                                        tcAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTG‑AACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACttt                                              <  1:1188321/124‑1 (MQ=255)
                                                                                        tcAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTG‑AACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACttt                                              >  2:1188321/1‑124 (MQ=255)
                                                                                        tcAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGTGTGCAGCGTa                               >  2:482940/1‑140 (MQ=255)
                                                                                              gccgccgccCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGTGTGCAGCGTACCGAtt                         <  2:192221/140‑1 (MQ=255)
                                                                                                 gccgccCGCGTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGTGTGCAGCGTACCGATTTAg                      >  1:909101/1‑140 (MQ=255)
                                                                                                       cgcgTCACTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGTGTGCAGCGTACCGATTTAGCCCTTg                <  1:136090/140‑1 (MQ=255)
                                                                                                              cTTTCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGTGTGCAGCGTACCGATTTAGCCCTTGCGCTGGc         <  1:355707/140‑1 (MQ=255)
                                                                                                               tttCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGTGTGCAGCGTACCGATTTAGCCCTTGCGCTGGCa        <  2:53169/140‑1 (MQ=255)
                                                                                                               tttCTCCGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGTGTGCAGCGTACCGATTTAGCCCTTGCGCTGGCa        <  1:571655/140‑1 (MQ=255)
                                                                                                                    ccGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGGATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGTGTGCAGCGTACCGATTTAGCCCTTGCGCTGGCAGCCtt   >  1:120524/1‑140 (MQ=255)
                                                                                                                    ccGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGTGTGCAGCGTACCGATTTAGCCCTTGCGCTgg          <  2:326730/133‑1 (MQ=255)
                                                                                                                    ccGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGTGTGCAGCGTACCGATTTAGCCCTTGCGCTgg          >  1:326730/1‑133 (MQ=255)
                                                                                                                    ccGGCACCTTTGCCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGTGTGCAGCGTACCGA‑TTAGCCCTTGCGCTGGCAGCCTtg  >  2:1090452/1‑140 (MQ=255)
                                                                                                                               |                                                                                                                                 
CTTTCGCTGATGTAGTGGAAGAGTACCAGCAATACCGCGAAAGCGAAGCCTGGCAGCGTGATGCGGCATTCTGGGCAGAACAGCGTCGTCAACTGCCGCCGCCCGCGTCACTTTCTCCGGCACCTTTACCGGGGCGCAGCGCCTCGGCAGATATTCTGCGCCTGAAACTGGAATTTACCGACGGGGAATTCCGCCAGCTGGCTACGCAACTTTCAGGTGTGCAGCGTACCGATTTAGCCCTTGCGCTGGCAGCCTTG  >  NC_000913/614674‑614930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A