Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 162 86.7 2935410 99.3% 2914862 138.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,148,135 T→C L80P (CTG→CCG)  gpr → L‑glyceraldehyde 3‑phosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,148,1350TC100.0% 261.6 / NA 76L80P (CTG→CCG) gprL‑glyceraldehyde 3‑phosphate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/35);  total (41/35)

CAATTTCGGTCACGTTAACGCGCTGGAATCACAGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAG  >  NC_000913/3147998‑3148271
                                                                                                                                         |                                                                                                                                        
caATTTCGGTCACGTTAACGCGCTGGAATCACAGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCccgc                                                                                                                                        >  1:275166/1‑140 (MQ=255)
   tttCGGTCACGTTAACGCGCTGGAATCACAGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCcgctgc                                                                                                                                     >  1:1223372/1‑140 (MQ=255)
        gTCACGTTAACGCGCTGGAATCACAGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCgg                                                                                                                                   >  2:1403524/1‑137 (MQ=255)
        gTCACGTTAACGCGCTGGAATCACAGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCgg                                                                                                                                   <  1:1403524/137‑1 (MQ=255)
             gTTAACGCGCTGGAATCACAGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGAttt                                                                                                                           >  1:45142/1‑140 (MQ=255)
                 aCGCGCTGGAATCACAGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGcc                                                                                                                       >  1:571837/1‑140 (MQ=255)
                      cTGGAATCACAGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTa                                                                                                                  <  2:45142/140‑1 (MQ=255)
                           aTCACAGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATcgcg                                                                                                             <  1:1006419/140‑1 (MQ=255)
                           aTCACAGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATcgcg                                                                                                             <  1:953482/140‑1 (MQ=255)
                           aTCACAGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATcgcg                                                                                                             <  1:790835/140‑1 (MQ=255)
                           aTCACAGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATcgcg                                                                                                             <  2:275166/140‑1 (MQ=255)
                           aTCACAGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATcgcg                                                                                                             <  1:1306049/140‑1 (MQ=255)
                           aTCACAGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATcgcg                                                                                                             >  2:790835/1‑140 (MQ=255)
                           aTCACAGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTCTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATcgcg                                                                                                             <  2:1159608/140‑1 (MQ=255)
                            tCACAGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGa                                                                                                            >  1:871379/1‑140 (MQ=255)
                                aGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGaa                                                                                                        <  1:970376/140‑1 (MQ=255)
                                aGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGaa                                                                                                        >  2:970376/1‑140 (MQ=255)
                                    tGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTATGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGa                                                                                                    >  2:633497/1‑140 (MQ=255)
                                    tGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGaa                                                                                                        >  1:191213/1‑136 (MQ=255)
                                    tGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGaa                                                                                                        <  2:191213/136‑1 (MQ=255)
                                    tGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGa                                                                                                    <  1:902339/140‑1 (MQ=255)
                                    tGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGa                                                                                                    >  1:1022723/1‑140 (MQ=255)
                                    tGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGa                                                                                                    >  2:191718/1‑140 (MQ=255)
                                         tCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTAtc                                                                                               <  1:1327225/140‑1 (MQ=255)
                                          ccTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGGCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGaa                                                                                                        >  2:659512/1‑130 (MQ=255)
                                          ccTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGGCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGaa                                                                                                        <  1:659512/130‑1 (MQ=255)
                                            tGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATctct                                                                                            >  1:704681/1‑140 (MQ=255)
                                              cGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTAc                                                                                          >  1:829467/1‑140 (MQ=255)
                                              cGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTAc                                                                                          >  2:1007064/1‑140 (MQ=255)
                                                tcaaGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTccacca                                                                                        <  2:998662/138‑1 (MQ=255)
                                                   aGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGa                                                                                                    <  1:1027746/125‑1 (MQ=255)
                                                   aGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGa                                                                                                    >  2:1027746/1‑125 (MQ=255)
                                                   aGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGAt                                                                                                   >  1:657289/1‑126 (MQ=255)
                                                   aGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGAt                                                                                                   <  2:657289/126‑1 (MQ=255)
                                                   aGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAgg                                                                                     >  2:886959/1‑140 (MQ=255)
                                                   aGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAgg                                                                                     >  2:1162416/1‑140 (MQ=255)
                                                     cGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAggct                                                                                   >  1:585201/1‑140 (MQ=255)
                                                      gTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAggctg                                                                                  <  1:144681/140‑1 (MQ=255)
                                                      gTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAggctg                                                                                  <  2:1197254/140‑1 (MQ=255)
                                                      gTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAggctg                                                                                  <  2:571837/140‑1 (MQ=255)
                                                        ttGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAggctggc                                                                                >  1:348479/1‑140 (MQ=255)
                                                         tGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAggctggct                                                                               <  1:423328/140‑1 (MQ=255)
                                                            tttGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACg                                                                            <  1:1007064/140‑1 (MQ=255)
                                                            tttGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACg                                                                            <  1:682449/140‑1 (MQ=255)
                                                                ggCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGatat                                                                        <  2:1022723/140‑1 (MQ=255)
                                                                     tACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTggc                                                                   >  1:1040808/1‑140 (MQ=255)
                                                                     tACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTggc                                                                   >  1:607442/1‑140 (MQ=255)
                                                                       cGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTggccc                                                                 <  2:871379/140‑1 (MQ=255)
                                                                            tttGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTggcccggccc                                                            <  1:191718/140‑1 (MQ=255)
                                                                               gATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTa                                                         <  2:829467/140‑1 (MQ=255)
                                                                                aTTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCtt                                                          <  2:835836/138‑1 (MQ=255)
                                                                                aTTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCtt                                                          >  1:835836/1‑138 (MQ=255)
                                                                                 tttAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACg                                                       >  1:602302/1‑140 (MQ=255)
                                                                                      ccAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGctct                                                  >  1:202224/1‑140 (MQ=255)
                                                                                        aacaaTTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTgg                                                <  1:804678/140‑1 (MQ=255)
                                                                                        aacaaTTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTgg                                                <  1:633497/140‑1 (MQ=255)
                                                                                          caaTTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCg                                              >  1:511614/1‑140 (MQ=255)
                                                                                          caaTTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCg                                              >  2:510500/1‑140 (MQ=255)
                                                                                          caaTTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACCTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCg                                              >  1:1342660/1‑140 (MQ=255)
                                                                                           aaTTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCgg                                             <  1:237248/140‑1 (MQ=255)
                                                                                                   gccgccTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTa                                     >  1:511144/1‑140 (MQ=255)
                                                                                                     cgccTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTaaa                                   >  1:1401485/1‑140 (MQ=255)
                                                                                                       ccTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATa                                 >  2:1148289/1‑140 (MQ=255)
                                                                                                       ccTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATa                                 >  2:328345/1‑140 (MQ=255)
                                                                                                        cTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATAc                                >  1:1178046/1‑140 (MQ=255)
                                                                                                          ccAGGAAGCGCAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCt                              <  2:202224/140‑1 (MQ=255)
                                                                                                                  cgcAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTg                             <  1:1313184/133‑1 (MQ=255)
                                                                                                                  cgcAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTg                             >  2:1313184/1‑133 (MQ=255)
                                                                                                                  cgcAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCa                      >  2:392986/1‑140 (MQ=255)
                                                                                                                  cgcAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCa                      >  1:814774/1‑140 (MQ=255)
                                                                                                                  cgcAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCa                      >  1:1272148/1‑140 (MQ=255)
                                                                                                                  cgcAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCa                      >  1:1265254/1‑140 (MQ=255)
                                                                                                                  cgcAGAAGAGAACTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCa                      >  1:120030/1‑140 (MQ=255)
                                                                                                                            aaCTTTGGTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCa            >  1:355701/1‑140 (MQ=255)
                                                                                                                                   gTCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTg     >  2:1400535/1‑140 (MQ=255)
                                                                                                                                    tCGCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGa    <  2:329282/140‑1 (MQ=255)
                                                                                                                                      gCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAg  <  2:1040808/140‑1 (MQ=255)
                                                                                                                                      gCCCGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAg  <  2:511144/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CAATTTCGGTCACGTTAACGCGCTGGAATCACAGCGTGCGATCCTGCGTAAAGCGTTTGATTTGGGCATTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAG  >  NC_000913/3147998‑3148271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A