Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
162 |
86.7 |
2935410 |
99.3% |
2914862 |
138.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,389,327 |
(C)6→7 |
coding (44/3324 nt) |
mscM ← |
miniconductance mechanosensitive channel MscM |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,389,321 | 1 | . | C | 100.0%
| 148.4
/ NA
| 44 | A17G (GCG→GGG) | mscM | miniconductance mechanosensitive channel MscM |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (19/25); total (19/25) |
GAACCTTTTCGTTCCTCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACG‑CCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATGGATTAGACCTGGTCTTTTTTGTCGTCAACCAATGGGCTGGCGTCGTGTTCTGCTTCGAT > NC_000913/4389186‑4389448
|
gAACCTTTTCGTTCCTCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACG‑cccc < 2:1245365/140‑1 (MQ=255)
aCCTTTTCGTTCCTCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACG‑cccccc > 1:588538/1‑140 (MQ=255)
ccTTTTCGTTCCTCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACG‑ccccccc > 2:389094/1‑139 (MQ=255)
ccTTTTCGTTCCTCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACG‑ccccccc < 1:547182/140‑2 (MQ=255)
ccTTTTCGTTCCTCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACG‑ccccccc < 1:893840/140‑2 (MQ=255)
ccTTTTCGTTCCTCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACG‑ccccccc > 1:303939/1‑139 (MQ=255)
ccTTTTCGTTCCTCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACG‑ccccccc > 2:893840/1‑139 (MQ=255)
ccTTTTCGTTCCTCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACG‑c < 2:660722/134‑1 (MQ=255)
ccTTTTCGTTCCTCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACG‑c > 1:660722/1‑134 (MQ=255)
cTTTTCGTTCCTCAAGCGCATTCAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACG‑ccccccca < 1:1041481/140‑3 (MQ=255)
cGTTCCTCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGa < 2:503886/140‑1 (MQ=255)
gTTCCTCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACG‑c < 1:305175/127‑1 (MQ=255)
gTTCCTCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACG‑c > 2:305175/1‑127 (MQ=255)
cTCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACG‑cccc > 1:81228/1‑126 (MQ=255)
cTCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACG‑cccc < 2:81228/126‑1 (MQ=255)
cTCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCAGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCAc > 2:954102/1‑140 (MQ=255)
tCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCAcc > 2:987332/1‑140 (MQ=255)
ttAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAg > 1:877502/1‑132 (MQ=255)
ttAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAg < 2:877502/132‑1 (MQ=255)
gaatGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGAGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGa > 2:392679/4‑140 (MQ=255)
aCTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGAt > 1:1421393/1‑140 (MQ=255)
aCTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGAt > 2:719459/1‑140 (MQ=255)
aGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCa > 2:309608/1‑140 (MQ=255)
gcCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGgc < 1:389094/140‑1 (MQ=255)
ccTCTACGACTTCCGGCTGTGCGGGTTTCGCAGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGgcg < 1:954102/140‑1 (MQ=255)
ctctACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGgcgc > 1:1167071/1‑140 (MQ=255)
ctctACGACTTCCGGCTGTGCGGGTTTCCCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGgcgc > 1:782636/1‑140 (MQ=255)
ctACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCAc > 2:820774/1‑140 (MQ=255)
cGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTc < 1:719459/140‑1 (MQ=255)
ttCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCg < 1:820774/140‑1 (MQ=255)
tctggCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGt > 1:708467/4‑140 (MQ=255)
tCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGt > 2:583212/1‑140 (MQ=255)
tCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGt > 2:1232400/1‑140 (MQ=255)
tCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGt < 2:303939/140‑1 (MQ=255)
tCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGt < 2:487547/140‑1 (MQ=255)
ggCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTc > 2:947216/1‑140 (MQ=255)
tgCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTg < 2:238720/140‑1 (MQ=255)
gCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGa < 2:798371/140‑1 (MQ=255)
gCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGa < 2:56200/140‑1 (MQ=255)
gCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGa < 2:912760/140‑1 (MQ=255)
gCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGa < 2:1167071/140‑1 (MQ=255)
gCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGa < 1:987332/140‑1 (MQ=255)
gCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGa < 1:947216/140‑1 (MQ=255)
tttCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATGGAt < 1:1164632/140‑1 (MQ=255)
tGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATGGATTAGACCTGGTCttttt < 1:543200/140‑1 (MQ=255)
tCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATGGATTAGACCTGGTCTTTTTTgt > 1:711192/1‑140 (MQ=255)
ccTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATGGATTAGACCTGGTCTTTTTTGTCGTCAAc > 1:224745/1‑140 (MQ=255)
tGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATGGATTAGACCTGGTCTTTTTTGTCGTCAACCa < 2:1421393/140‑1 (MQ=255)
tGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATGGATTAGACCTGGTCTTTTTTGTCGTCAACCa > 1:1082937/1‑140 (MQ=255)
tGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATGGATTAGACCTGGTCTTTTTTGTCGTCAACCa > 2:897837/1‑140 (MQ=255)
tGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATGGATTAGACCTGGTCTTTTTTGTCGTCAACCa < 2:1082937/140‑1 (MQ=255)
gAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATGGATTAGACCTGGTCTTTTTTGTCGTCAACCaa > 1:787610/1‑140 (MQ=255)
ttgtttgCTATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATGGATTAGACCTGGTCTTTTTTGTCGTCAACCAATGGGCTg < 2:787610/140‑1 (MQ=255)
tATCGGGGGCCGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATAGATTAGACCTGGTCTTTTTTGTCGTCAACCAATGGGCTGGCGTCgtg < 1:1232400/140‑1 (MQ=255)
cGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATGGATTAGACCTGGTCTTTTTTGTCGTCAACCAATGGGCTGGCGTCGTGTTCTGCTTCg < 2:711192/140‑1 (MQ=255)
cGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATGGATTAGACCTGGTCTTTTTTGTCGTCAACCAATGGGCTGGCGTCGTGTTCTGCTTCg < 2:480403/140‑1 (MQ=255)
cGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATGGATTAGACCTGGTCTTCTTTGTCGTCAACCAATGGGCTg < 2:112782/122‑1 (MQ=255)
cGTCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATGGATTAGACCTGGTCTTCTTTGTCGTCAACCAATGGGCTg > 1:112782/1‑122 (MQ=255)
tCGCGGCGTACGCCCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATGGATTAGACCTGGTCTTTTTTGTCGTCAACCAATGGGCTGGCGTCGTGTTCTGCTTCGAt < 2:1177986/140‑1 (MQ=255)
|
GAACCTTTTCGTTCCTCAAGCGCATTTAAGGCAGACTGGAGCGCCTCTACGACTTCCGGCTGTGCGGGTTTCGCCGCTTTTGCCTGCTCCAGTTCCTGAGTGATTTGTTTGCTATCGGGGGCCGTCGCGGCGTACG‑CCCCCCAACTGAGGCACCAGGCCATCAGAAAAGTGATAATCAGGCGCACGTCAGCGTTTCCTTTGATGGATTAGACCTGGTCTTTTTTGTCGTCAACCAATGGGCTGGCGTCGTGTTCTGCTTCGAT > NC_000913/4389186‑4389448
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
