Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	121	68.4	2351762	99.3%	2335299	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,805,945	A→G	Q377Q (CAA→CAG)	proV →	glycine betaine ABC transporter ATP binding subunit ProV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,805,945	0	A	G	100.0%	169.1 / NA	47	Q377Q (CAA→CAG)	proV	glycine betaine ABC transporter ATP binding subunit ProV
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/30); total (17/30)

ACGCAGCAGCAAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCACGCCAGCGGCGGACAGTGCCGCGCAATCCGCAGACGCC  >  NC_000913/2805814‑2806078

aCGCAGCAGCAAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGt                                                                                                                               <  2:678219/140‑1 (MQ=255)

aCGCAGCAGCAAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGt                                                                                                                               <  2:391748/140‑1 (MQ=255)

 cGCAGCAGCAAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTc                                                                                                                              <  2:910484/140‑1 (MQ=255)

    agcagcaAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGc                                                                                                                           <  1:878688/140‑1 (MQ=255)

     gcagcaAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGGATGTCGGca                                                                                                                          >  1:1067756/1‑140 (MQ=255)

      cagcaAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGcat                                                                                                                         >  1:478084/1‑140 (MQ=255)

           aGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGc                                                                                                                           <  1:1077092/133‑1 (MQ=255)

           aGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGc                                                                                                                           >  2:1077092/1‑133 (MQ=255)

                        gcgcTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAAtgct                                                                                                       >  2:1015411/1‑140 (MQ=255)

                         cgcTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAAtgctg                                                                                                      <  2:478084/140‑1 (MQ=255)

                             gattgatGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAAtgctgctgc                                                                                                  <  2:458388/140‑1 (MQ=255)

                             gattgatGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAAtgctgctgc                                                                                                  <  1:746590/140‑1 (MQ=255)

                               ttgatGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTgcgc                                                                                                <  1:1130675/140‑1 (MQ=255)

                                   tGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCtt                                                                                            >  1:518268/1‑140 (MQ=255)

                                    gcgcCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCttt                                                                                           <  2:474798/140‑1 (MQ=255)

                                     cgcCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTa                                                                                          <  1:1032950/140‑1 (MQ=255)

                                     cgcCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTa                                                                                          >  1:397284/1‑140 (MQ=255)

                                     cgcCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTa                                                                                          <  1:449109/140‑1 (MQ=255)

                                          ttAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCg                                                                                     >  2:287920/1‑140 (MQ=255)

                                            aGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCGCCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTg                                                                                   <  2:83392/140‑1 (MQ=255)

                                              cAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAg                                                                                 <  2:518268/140‑1 (MQ=255)

                                              cAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAg                                                                                 <  1:287920/140‑1 (MQ=255)

                                               aGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAgg                                                                                <  1:738270/140‑1 (MQ=255)

                                                  cGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAggggg                                                                             <  2:954803/140‑1 (MQ=255)

                                                  cGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAggggg                                                                             <  2:129080/140‑1 (MQ=255)

                                                  cGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAggggg                                                                             >  1:587517/1‑140 (MQ=255)

                                                    aTGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAg                                                                                 >  2:587880/1‑134 (MQ=255)

                                                    aTGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAg                                                                                 <  1:587880/134‑1 (MQ=255)

                                                    aTGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTa                                                                           <  1:761694/140‑1 (MQ=255)

                                                           aaCGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATg                                                                    >  1:118225/1‑140 (MQ=255)

                                                            aCGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATgg                                                                   <  2:854526/140‑1 (MQ=255)

                                                            aCGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATgg                                                                   <  2:930162/140‑1 (MQ=255)

                                                              gCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCt                                                                 <  1:1159502/140‑1 (MQ=255)

                                                                 tctTAGCGAGTTGCTCTCTCATGTCGGACAGGCGCCCTGTGCGGTGCCCGTTGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGAt                                                              <  2:773153/140‑1 (MQ=255)

                                                                  ctTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATc                                                             >  1:18622/1‑140 (MQ=255)

                                                                          gTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAaataat                                                     <  2:18622/140‑1 (MQ=255)

                                                                           ttGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCCTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATc                                                    <  1:788654/140‑1 (MQ=255)

                                                                              ctctctCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGt                                                 >  1:1023639/1‑140 (MQ=255)

                                                                                 tctcATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTggg                                              >  1:777725/1‑140 (MQ=255)

                                                                                        tCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCAc                                       >  2:1084771/1‑140 (MQ=255)

                                                                                        tCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGAAAATAATGGCTGATCAAAATAATCCGTGGGATACAAc                                       >  1:462728/1‑140 (MQ=255)

                                                                                             cAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCACGCCAg                                  >  2:438255/1‑140 (MQ=255)

                                                                                                           ggTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCACGCCAGCGGCGGACAGTGcc                    <  1:644250/140‑1 (MQ=255)

                                                                                                                        gacgaGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCACGCCAGCGGCGGACAGTGCCGCGCAATCCGCAg       <  2:1132336/140‑1 (MQ=255)

                                                                                                                         acgaGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCACGCCAGCGGCGGACAGTGCCGCGCaa              >  1:594292/1‑132 (MQ=255)

                                                                                                                         acgaGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCACGCCAGCGGCGGACAGTGCCGCGCaa              <  2:594292/132‑1 (MQ=255)

                                                                                                                             ggACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCACGCCAGCGGCGGACAGTGCCGCGCAATCCGCAGACGcc  <  2:507984/140‑1 (MQ=255)

ACGCAGCAGCAAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCACGCCAGCGGCGGACAGTGCCGCGCAATCCGCAGACGCC  >  NC_000913/2805814‑2806078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A