Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 121 68.4 2351762 99.3% 2335299 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,829,459 T→C I138T (ATC→ACC)  srlR → DNA‑binding transcriptional repressor SrlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,829,4590TC98.3% 214.2 / ‑4.3 60I138T (ATC→ACC) srlRDNA‑binding transcriptional repressor SrlR
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (35/24);  total (36/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.44e-01

CGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAAC  >  NC_000913/2829322‑2829594
                                                                                                                                         |                                                                                                                                       
cGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTAccc                                                                                                                                       >  2:65634/1‑140 (MQ=255)
cGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTAccc                                                                                                                                       >  1:955780/1‑140 (MQ=255)
       aTCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGcc                                                                                                                                <  1:588252/140‑1 (MQ=255)
       aTCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGcc                                                                                                                                >  2:781132/1‑140 (MQ=255)
         cATTCTTGATGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAg                                                                                                                              >  1:179035/1‑140 (MQ=255)
                  tGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGt                                                                                                                     >  1:1080446/1‑140 (MQ=255)
                      ggCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCg                                                                                                                 >  2:623366/1‑140 (MQ=255)
                      ggCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCg                                                                                                                 >  1:450951/1‑140 (MQ=255)
                      ggCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCg                                                                                                                 >  2:1152224/1‑140 (MQ=255)
                                ttttGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCg                                                                                                       >  2:324707/1‑140 (MQ=255)
                                ttttGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCg                                                                                                       >  2:11773/1‑140 (MQ=255)
                                 tttGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCgg                                                                                                      <  2:779531/140‑1 (MQ=255)
                                 tttGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCgg                                                                                                      <  1:1152224/140‑1 (MQ=255)
                                    gCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCt                                                                                                   <  1:781132/140‑1 (MQ=255)
                                    gCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCt                                                                                                   <  2:855218/140‑1 (MQ=255)
                                     cAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTc                                                                                                  >  2:635129/1‑140 (MQ=255)
                                        aTGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCGACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCAtt                                                                                               >  1:576870/1‑140 (MQ=255)
                                          ggTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTc                                                                                             >  2:29943/1‑140 (MQ=255)
                                                  tGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCaa                                                                                     <  1:312806/140‑1 (MQ=255)
                                                  tGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCaa                                                                                     <  1:635129/140‑1 (MQ=255)
                                                    ctctCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACt                                                                                   >  1:349888/1‑140 (MQ=255)
                                                    ctctCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACt                                                                                   >  1:40661/1‑140 (MQ=255)
                                                      ctcGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTgg                                                                                 >  1:458414/1‑140 (MQ=255)
                                                      ctcGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTgg                                                                                 <  1:623366/140‑1 (MQ=255)
                                                         gcgcTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCag                                                                              >  2:132625/1‑140 (MQ=255)
                                                          cgcTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCaga                                                                             <  1:29943/140‑1 (MQ=255)
                                                              ttAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAAt                                                                         <  2:1085326/140‑1 (MQ=255)
                                                                       tCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAg                                                                >  1:1043617/1‑140 (MQ=255)
                                                                       tCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCAAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCt                                                                     >  1:1044838/1‑135 (MQ=255)
                                                                       tCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCAAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCt                                                                     <  2:1044838/135‑1 (MQ=255)
                                                                       tCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCAAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCt                                                                     <  2:213159/135‑1 (MQ=255)
                                                                       tCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCAAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCt                                                                     >  1:213159/1‑135 (MQ=255)
                                                                         aCGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCa                                                              >  1:1145726/1‑140 (MQ=255)
                                                                                tgaCCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCAcc                                                       >  1:16744/1‑140 (MQ=255)
                                                                                tgaCCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCAcc                                                       >  1:277275/1‑140 (MQ=255)
                                                                                  aCCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCtt                                                     >  2:528973/1‑140 (MQ=255)
                                                                                      aCAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGAt                                                 <  1:528973/140‑1 (MQ=255)
                                                                                      aCAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGAt                                                 >  1:377113/1‑140 (MQ=255)
                                                                                       cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATa                                                >  1:198941/1‑140 (MQ=255)
                                                                                       cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATa                                                >  2:1054152/1‑140 (MQ=255)
                                                                                       cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATa                                                >  1:759349/1‑140 (MQ=255)
                                                                                          ccTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAAt                                             <  1:777216/140‑1 (MQ=255)
                                                                                              cATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGtt                                         >  1:101502/1‑140 (MQ=255)
                                                                                                tatCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTa                                       <  2:388051/140‑1 (MQ=255)
                                                                                                    gTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATggg                                   <  2:179035/140‑1 (MQ=255)
                                                                                                    gTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATggg                                   >  2:509760/1‑140 (MQ=255)
                                                                                                      cAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCa                                 >  2:37426/1‑140 (MQ=255)
                                                                                                        aTGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCAcc                               >  1:689665/1‑140 (MQ=255)
                                                                                                            gcTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACg                           <  2:16744/140‑1 (MQ=255)
                                                                                                             cTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACgg                          >  1:1086026/1‑140 (MQ=255)
                                                                                                               aTCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCa                        >  2:107354/1‑140 (MQ=255)
                                                                                                                tCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCAt                       <  2:902893/140‑1 (MQ=255)
                                                                                                                tCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCAt                       <  1:1166492/140‑1 (MQ=255)
                                                                                                                tCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCAt                       <  2:40661/140‑1 (MQ=255)
                                                                                                                tCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCAt                       <  1:132625/140‑1 (MQ=255)
                                                                                                                     aCTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGAtc                  >  2:766029/1‑140 (MQ=255)
                                                                                                                          aTAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAAt             >  2:88291/1‑140 (MQ=255)
                                                                                                                           tAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATg            <  2:416136/140‑1 (MQ=255)
                                                                                                                              cGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCgg         >  1:820647/1‑140 (MQ=255)
                                                                                                                                   aaaCTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTa    >  2:334129/1‑140 (MQ=255)
                                                                                                                                     aCTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAac  <  1:1068584/140‑1 (MQ=255)
                                                                                                                                     aCTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAac  <  1:65634/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAAC  >  NC_000913/2829322‑2829594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A