Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 121 68.4 2351762 99.3% 2335299 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,158,512 G→A R288H (CGC→CAC)  yqhG → DUF3828 domain‑containing protein YqhG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,158,5120GA100.0% 166.2 / NA 49R288H (CGC→CAC) yqhGDUF3828 domain‑containing protein YqhG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/31);  total (18/31)

AGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTT  >  NC_000913/3158384‑3158639
                                                                                                                                |                                                                                                                               
aGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAg                                                                                                                        <  2:669895/138‑1 (MQ=255)
aGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAg                                                                                                                        >  1:669895/1‑138 (MQ=255)
aGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCg                                                                                                                      >  2:224739/1‑140 (MQ=255)
 gTCGTGGAAAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCgg                                                                                                                     <  2:132812/140‑1 (MQ=255)
 gTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCgg                                                                                                                     <  2:763251/140‑1 (MQ=255)
             aCCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTaa                                                                                                         <  2:1034932/140‑1 (MQ=255)
                 ccgATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAg                                                                                                     >  2:102387/1‑140 (MQ=255)
                 ccgATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAg                                                                                                     >  2:118283/1‑140 (MQ=255)
                  cgATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGa                                                                                                    <  2:456178/140‑1 (MQ=255)
                               aTTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCT‑CTTAAGCAGATTGAGGCAAAAACt                                                                                      >  2:197541/1‑140 (MQ=255)
                                     gTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTgccgc                                                                                 <  1:224739/140‑1 (MQ=255)
                                      ttCGTTTTACGATGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTgccgcc                                                                                <  2:1034896/140‑1 (MQ=255)
                                         gTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGa                                                                             <  1:657966/140‑1 (MQ=255)
                                         gTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGa                                                                             <  2:838026/140‑1 (MQ=255)
                                           tttACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGAtt                                                                           >  1:15824/1‑140 (MQ=255)
                                            ttACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTa                                                                          <  2:46583/140‑1 (MQ=255)
                                                 cTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCa                                                                     <  2:805855/140‑1 (MQ=255)
                                                      cAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAg                                                                <  1:102387/140‑1 (MQ=255)
                                                      cAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAg                                                                <  2:245470/140‑1 (MQ=255)
                                                      cAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAg                                                                <  2:541842/140‑1 (MQ=255)
                                                      cAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAg                                                                <  2:619640/140‑1 (MQ=255)
                                                      cAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAg                                                                <  2:790527/140‑1 (MQ=255)
                                                      cAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAg                                                                <  1:979149/140‑1 (MQ=255)
                                                      cAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAg                                                                <  1:295088/140‑1 (MQ=255)
                                                         tGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTg                                                             >  1:1014687/1‑140 (MQ=255)
                                                         tGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTg                                                             >  2:655668/1‑140 (MQ=255)
                                                           gCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGaa                                                           <  2:15824/140‑1 (MQ=255)
                                                              acaacGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTa                                                        >  2:658821/1‑140 (MQ=255)
                                                                 acGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCATATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAAt                                                     <  1:12168/140‑1 (MQ=255)
                                                                 acGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAAt                                                     <  1:196139/140‑1 (MQ=255)
                                                                  cGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATa                                                    >  2:93355/1‑140 (MQ=255)
                                                                         cAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTa                                             >  2:820174/1‑140 (MQ=255)
                                                                           aGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGc                                           <  1:1168134/140‑1 (MQ=255)
                                                                              aCTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCgg                                        >  1:1081565/1‑140 (MQ=255)
                                                                                tGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGaa                                      <  2:798111/140‑1 (MQ=255)
                                                                                  gTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACa                                    >  1:1047027/1‑140 (MQ=255)
                                                                                       ataGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACaa                                                >  2:924332/3‑123 (MQ=255)
                                                                                       ataGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACaa                                                <  1:924332/121‑1 (MQ=255)
                                                                                       aCAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACaataaa                               >  2:494896/1‑140 (MQ=255)
                                                                                            ggcgCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCgg                                        >  1:1074510/1‑126 (MQ=255)
                                                                                            ggcgCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCgg                                        <  2:1074510/126‑1 (MQ=255)
                                                                                               gcgAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAggg                       <  2:893549/140‑1 (MQ=255)
                                                                                                 gAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGaa                     <  2:460913/140‑1 (MQ=255)
                                                                                                 gAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGaa                     <  1:93355/140‑1 (MQ=255)
                                                                                                 gAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGaa                     <  1:820174/140‑1 (MQ=255)
                                                                                                 gAAGGCAAGTGCGAAATCGCTGATTTTATCCACGCGAACAGCGGCAGCCTACTTGAGCAGATTGAGGCAAATACTGACGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGaa                     <  2:775919/140‑1 (MQ=255)
                                                                                                   aGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAaca                   <  2:1014687/140‑1 (MQ=255)
                                                                                                     gCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACt                 <  1:658821/140‑1 (MQ=255)
                                                                                                                   gCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGAtt   >  1:747596/1‑140 (MQ=255)
                                                                                                                    cTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGAttt  >  1:590565/1‑140 (MQ=255)
                                                                                                                                |                                                                                                                               
AGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTT  >  NC_000913/3158384‑3158639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A