Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	121	68.4	2351762	99.3%	2335299	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,283,280	C→T	I46I (ATC→ATT)	kbaY →	tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,283,280	0	C	T	100.0%	227.4 / NA	64	I46I (ATC→ATT)	kbaY	tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaY
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (31/33); total (31/33)

AGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTGGATGATATTC  >  NC_000913/3283146‑3283413

aGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGc                                                                                                                                  >  2:955323/1‑140 (MQ=255)

  cATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCg                                                                                                                                <  1:241220/140‑1 (MQ=255)

     tATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGaa                                                                                                                             >  2:547607/1‑140 (MQ=255)

      aTCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAAc                                                                                                                            >  1:905630/1‑140 (MQ=255)

            aCTAAATATCTGTTACAGGACGCCGAGGCCAATGCCTCCGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCggg                                                                                                                      >  2:788309/1‑140 (MQ=255)

            aCTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCggg                                                                                                                      >  2:102265/1‑140 (MQ=255)

            aCTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCggg                                                                                                                      <  1:50051/140‑1 (MQ=255)

              tAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGa                                                                                                                    <  1:945485/140‑1 (MQ=255)

              tAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGa                                                                                                                    <  2:365283/140‑1 (MQ=255)

              tAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGa                                                                                                                    <  2:1054596/140‑1 (MQ=255)

                 atatCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCt                                                                                                                 >  2:1117461/1‑140 (MQ=255)

                     cTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTaa                                                                                                             <  1:955323/140‑1 (MQ=255)

                           cAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACAt                                                                                                       <  1:499724/140‑1 (MQ=255)

                              gACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCggg                                                                                                                      >  2:988050/1‑122 (MQ=255)

                              gACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCggg                                                                                                                      <  1:988050/122‑1 (MQ=255)

                               aCGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATcgcg                                                                                                   <  2:923629/140‑1 (MQ=255)

                                cGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCgcgc                                                                                                  >  1:85619/1‑140 (MQ=255)

                                  cccAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTg                                                                                                >  2:404866/1‑140 (MQ=255)

                                  cccAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTg                                                                                                >  2:450687/1‑140 (MQ=255)

                                  cccAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTg                                                                                                >  2:58370/1‑140 (MQ=255)

                                     aGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGaa                                                                                             <  1:547607/140‑1 (MQ=255)

                                      ggCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGTCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAag                                                                                            >  2:284071/1‑140 (MQ=255)

                                      ggCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAag                                                                                            <  1:58370/140‑1 (MQ=255)

                                       gCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAaga                                                                                           <  1:1117461/140‑1 (MQ=255)

                                        ccAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCACGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTAGAagag                                                                                          >  2:477529/1‑140 (MQ=255)

                                            tGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCt                                                                                      >  2:558498/1‑140 (MQ=255)

                                              gCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTAc                                                                                    <  1:637302/140‑1 (MQ=255)

                                                       tGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTgtgt                                                                           >  2:213941/1‑140 (MQ=255)

                                                        gCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTa                                                                          <  1:865638/140‑1 (MQ=255)

                                                          cTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGTCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAgc                                                                        <  1:284071/140‑1 (MQ=255)

                                                            gCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAgcgc                                                                      <  1:63713/140‑1 (MQ=255)

                                                                    cATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCca                                                              <  2:814259/140‑1 (MQ=255)

                                                                    cATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCca                                                              <  2:1052517/140‑1 (MQ=255)

                                                                       tCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACaa                                                           >  2:785689/1‑140 (MQ=255)

                                                                        cATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAAc                                                          >  2:180796/1‑140 (MQ=255)

                                                                         aTAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAAcc                                                         <  2:352078/140‑1 (MQ=255)

                                                                             cGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTaca                                                     <  1:293479/140‑1 (MQ=255)

                                                                               ccGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTacaac                                                   >  2:263476/1‑140 (MQ=255)

                                                                                 gagaCGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCca                                                              >  1:509342/1‑127 (MQ=255)

                                                                                 gagaCGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCca                                                              <  2:509342/127‑1 (MQ=255)

                                                                                  agaCGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATg                                                >  2:827059/1‑140 (MQ=255)

                                                                                               gATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGc                                   <  1:558498/140‑1 (MQ=255)

                                                                                                  ccTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCAtc                                >  2:203525/1‑140 (MQ=255)

                                                                                                  ccTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCAtc                                >  2:906696/1‑140 (MQ=255)

                                                                                                  ccTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCAtc                                >  2:1086791/1‑140 (MQ=255)

                                                                                                      gAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGa                            >  2:606541/1‑140 (MQ=255)

                                                                                                            tgCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGac                           >  2:240231/1‑135 (MQ=255)

                                                                                                            tgCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGac                           <  1:240231/135‑1 (MQ=255)

                                                                                                            tgCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGAccacca                      >  2:902378/1‑140 (MQ=255)

                                                                                                            tgCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGAccacca                      >  2:418812/1‑140 (MQ=255)

                                                                                                                 tGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAAt                 <  1:263476/140‑1 (MQ=255)

                                                                                                                 tGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAAt                 <  1:785689/140‑1 (MQ=255)

                                                                                                                   aaaTGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCg               <  1:477529/140‑1 (MQ=255)

                                                                                                                    aaTGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGa                   <  1:692506/135‑1 (MQ=255)

                                                                                                                    aaTGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGa                   >  2:692506/1‑135 (MQ=255)

                                                                                                                    aaTGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGc              >  2:421630/1‑140 (MQ=255)

                                                                                                                     aTGCGATCGCCGGTGATTCTCGCCGGACCGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCt             <  1:788309/140‑1 (MQ=255)

                                                                                                                     aTGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCt             <  1:102265/140‑1 (MQ=255)

                                                                                                                      tGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTg            >  1:677421/1‑140 (MQ=255)

                                                                                                                      tGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTg            >  1:165780/1‑140 (MQ=255)

                                                                                                                       gCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTgg           <  1:902378/140‑1 (MQ=255)

                                                                                                                        cGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTGga          <  1:906696/140‑1 (MQ=255)

                                                                                                                           tCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCg               >  2:532800/1‑132 (MQ=255)

                                                                                                                           tCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCg               <  1:532800/132‑1 (MQ=255)

                                                                                                                              ccGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTGGATGatat    <  1:418812/140‑1 (MQ=255)

                                                                                                                                ggTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTGGATGATATTc  <  2:336321/140‑1 (MQ=255)

                                                                                                                                ggTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTGGATGATATTc  >  1:532744/1‑140 (MQ=255)

AGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTGGATGATATTC  >  NC_000913/3283146‑3283413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A