Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	120	93.5	3664254	88.3%	3235536	136.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,430,995	G→A	L649L (CTG→CTA)	stfR →	Rac prophage; putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,430,995	0	G	A	99.2%	426.7 / ‑4.5	121	L649L (CTG→CTA)	stfR	Rac prophage; putative membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (66/54); total (66/55)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 121 total aligned reads displayed.

CATGTTCTGGCAATATCAAAACAATGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTGTCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCTTGATTTTAAGAGTGGGCGTGGTTTTTATGAGT  >  NC_000913/1430860‑1431128

cATGTTCTGGCAATATCAAAACAATGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATc                                                                                                                                     >  1:145399/1‑138 (MQ=255)

 aTGTTCTGGCAATATCAAAACAATGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCa                                                                                                                                    >  2:1419752/1‑138 (MQ=255)

    ttCTGGCAATATCAAAACAATGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAata                                                                                                                                 >  2:309437/1‑138 (MQ=255)

          cAATATCAAAACAATGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCa                                                                                                                           <  1:1419752/138‑1 (MQ=255)

          cAATATCAAAACAATGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCa                                                                                                                           <  2:145399/138‑1 (MQ=255)

          cAATATCAAAACAATGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCa                                                                                                                           <  1:1193704/138‑1 (MQ=255)

           aaTATCAAAACAATGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCAc                                                                                                                          >  1:1402628/1‑138 (MQ=255)

                cAAAACAATGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGt                                                                                                                     >  2:1571706/1‑138 (MQ=255)

                cAAAACAATGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGt                                                                                                                     >  2:1208129/1‑138 (MQ=255)

                cAAAACAATGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGt                                                                                                                     >  2:227059/1‑138 (MQ=255)

                cAAAACAATGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGt                                                                                                                     <  2:349427/138‑1 (MQ=255)

                cAAAACAATGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGt                                                                                                                     >  2:770122/1‑138 (MQ=255)

                cAAAACAATGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGt                                                                                                                     >  2:786732/1‑138 (MQ=255)

                cAAAACAATGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGt                                                                                                                     >  1:589818/1‑138 (MQ=255)

                     cAATGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGa                                                                                                                <  1:791933/138‑1 (MQ=255)

                        tGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGAtct                                                                                                             <  1:770122/138‑1 (MQ=255)

                        tGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGAtct                                                                                                             <  2:1604921/138‑1 (MQ=255)

                          aaCGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCa                                                                                                                           >  1:551086/1‑122 (MQ=255)

                          aaCGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCa                                                                                                                           <  2:551086/122‑1 (MQ=255)

                          aaCGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGAtct                                                                                                             <  1:846562/136‑1 (MQ=255)

                          aaCGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGAtct                                                                                                             >  2:846562/1‑136 (MQ=255)

                          aaCGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCg                                                                                                           >  2:1366347/1‑138 (MQ=255)

                          aaCGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCg                                                                                                           >  1:1706123/1‑138 (MQ=255)

                                  aTTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAAt                                                                                                   >  1:1366522/1‑138 (MQ=255)

                                      aCTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATa                                                                                               <  2:1378247/138‑1 (MQ=255)

                                            taatGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCt                                                                                         >  1:1026218/1‑138 (MQ=255)

                                                   gaGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGtt                                                                                  >  2:1289364/1‑138 (MQ=255)

                                                   gaGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGtt                                                                                  >  2:616698/1‑138 (MQ=255)

                                                    aGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGa                                                                                                 <  2:1439477/122‑1 (MQ=255)

                                                    aGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGa                                                                                                 >  1:1439477/1‑122 (MQ=255)

                                                    aGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGttt                                                                                 >  1:659785/1‑138 (MQ=255)

                                                    aGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGttt                                                                                 >  2:945418/1‑138 (MQ=255)

                                                      taataaGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCt                                                                                         <  1:367900/128‑1 (MQ=255)

                                                      taataaGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTgg                                                                               >  2:1244198/1‑138 (MQ=255)

                                                      taataaGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTgg                                                                               >  2:450692/1‑138 (MQ=255)

                                                      taataaGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTgg                                                                               >  2:562421/1‑138 (MQ=255)

                                                      taataaGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTgg                                                                               >  2:980146/1‑138 (MQ=255)

                                                      taataaGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTgg                                                                               >  1:194490/1‑138 (MQ=255)

                                                        ataaGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGAcc                                                                                          >  2:908975/1‑125 (MQ=255)

                                                        ataaGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGAcc                                                                                          <  1:908975/125‑1 (MQ=255)

                                                        ataaGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTgggg                                                                             >  1:1340289/1‑138 (MQ=255)

                                                        ataaGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTgggg                                                                             >  2:1083823/1‑138 (MQ=255)

                                                                gatgatTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAAt                                                                     >  2:427755/1‑138 (MQ=255)

                                                                gatgatTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAAt                                                                     >  1:939651/1‑138 (MQ=255)

                                                                  tgatTTGCTCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATcc                                                                   >  1:1072875/1‑138 (MQ=255)

                                                                  tgatTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTGTCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATcc                                                                   <  1:1543105/138‑1 (MQ=255)

                                                                  tgatTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATcc                                                                   <  1:1208129/138‑1 (MQ=255)

                                                                  tgatTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATcc                                                                   >  2:1734309/1‑138 (MQ=255)

                                                                  tgatTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATcc                                                                   <  1:1571706/138‑1 (MQ=255)

                                                                  tgatTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATcc                                                                   >  2:1360678/1‑138 (MQ=255)

                                                                  tgatTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATcc                                                                   >  2:1349706/1‑138 (MQ=255)

                                                                  tgatTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATcc                                                                   >  1:484044/1‑138 (MQ=255)

                                                                  tgatTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATcc                                                                   >  2:1054186/1‑138 (MQ=255)

                                                                  tgatTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATcc                                                                   <  2:106878/138‑1 (MQ=255)

                                                                  tgatTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATcc                                                                   <  1:89899/138‑1 (MQ=255)

                                                                      ttGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCg                                                               <  1:786732/138‑1 (MQ=255)

                                                                              tgtgTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATg                                                       >  2:1497405/1‑138 (MQ=255)

                                                                                 gtTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTAt                                                    <  1:427755/138‑1 (MQ=255)

                                                                                     tATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATcgcg                                                <  1:1349706/138‑1 (MQ=255)

                                                                                     tATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATcgcg                                                <  2:1366522/138‑1 (MQ=255)

                                                                                     tATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATcgcg                                                >  1:1249093/1‑138 (MQ=255)

                                                                                      aTGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCgcgg                                               <  2:659785/138‑1 (MQ=255)

                                                                                          tGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACGAGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGa                                           >  2:1634829/1‑138 (MQ=255)

                                                                                          tGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATcgcg                                                >  1:1008810/1‑133 (MQ=255)

                                                                                          tGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATcgcg                                                <  1:1297582/133‑1 (MQ=255)

                                                                                          tGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATcgcg                                                >  2:1297582/1‑133 (MQ=255)

                                                                                          tGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATcgcg                                                <  2:1008810/133‑1 (MQ=255)

                                                                                          tGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGa                                           >  2:1704682/1‑138 (MQ=255)

                                                                                            aTGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACg                                         >  2:166798/1‑138 (MQ=255)

                                                                                            aTGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACg                                         >  1:1713525/1‑138 (MQ=255)

                                                                                            aTGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACg                                         >  2:474435/1‑138 (MQ=255)

                                                                                            aTGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACg                                         >  1:907212/1‑138 (MQ=255)

                                                                                            aTGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACg                                         >  1:1120365/1‑138 (MQ=255)

                                                                                            aTGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACg                                         >  2:1106192/1‑138 (MQ=255)

                                                                                            aTGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTACCGCGGCGGACg                                         >  1:1518952/1‑138 (MQ=255)

                                                                                              ggCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATcgcg                                                >  2:1103308/1‑129 (MQ=255)

                                                                                              ggCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGtt                                       >  2:1124411/1‑138 (MQ=255)

                                                                                              ggCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGAATCGCGGCGGACGtt                                       >  2:195759/1‑138 (MQ=255)

                                                                                                cgcTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTAt                                     <  2:954763/138‑1 (MQ=255)

                                                                                                cgcTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTAt                                     <  2:1072875/138‑1 (MQ=255)

                                                                                                  ctgctTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATcc                                   <  1:474435/138‑1 (MQ=255)

                                                                                                  ctgctTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATcc                                   <  1:309437/138‑1 (MQ=255)

                                                                                                  ctgctTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATcc                                   <  2:1706123/138‑1 (MQ=255)

                                                                                                   tgctTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCt                                  >  1:143110/1‑138 (MQ=255)

                                                                                                   tgctTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCt                                  >  2:157208/1‑138 (MQ=255)

                                                                                                   tgctTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCt                                  >  1:883683/1‑138 (MQ=255)

                                                                                                   tgctTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCt                                  >  2:1116896/1‑138 (MQ=255)

                                                                                                            tGTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCTTGATTTTaa                         >  1:1027315/1‑138 (MQ=255)

                                                                                                             gTTTTTGCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCTTGATTTTAag                        <  2:1713525/138‑1 (MQ=255)

                                                                                                                   gCGTTTATTGAAGATGGCCTATCAATATCCGCTCCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCTTGATTTTAAGAGTggg                  <  1:195759/138‑1 (MQ=255)

                                                                                                                   gCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCTTGATTTTAAGAGTggg                  <  2:1402628/138‑1 (MQ=255)

                                                                                                                   gCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCTTGATTTTAAGAGTggg                  <  1:327676/138‑1 (MQ=255)

                                                                                                                   gCGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCTTGATCTTAAGAGTggg                  <  1:993782/138‑1 (MQ=255)

                                                                                                                    cGTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCTTGATTTTAAGAGTGGGc                 >  1:1052131/1‑138 (MQ=255)

                                                                                                                     gTTTATTGAAGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCTTGATTTTAAGAGTGGGCg                <  1:1116896/138‑1 (MQ=255)

                                                                                                                             aaGATGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCTTGATTTTAAGAGTGGGCGTGGttttt        <  1:1620000/138‑1 (MQ=255)

                                                                                                                                aTGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCTTGATTTTAAGAGTGGGCGTGGTTTTTATg     <  2:194490/138‑1 (MQ=255)

                                                                                                                                aTGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCTTGATTTTAAGAGTGGGCGTGGTTTTTATg     <  1:267240/138‑1 (MQ=255)

                                                                                                                                aTGGCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCTTGATTTTAAGAGTGGGCGTGGTTTTTATg     <  1:1734309/138‑1 (MQ=255)

                                                                                                                                   gCCTATCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCTTGATTTTAAGAGTGGGCGTGGTTTTTATGAGt  <  1:1360678/138‑1 (MQ=255)

CATGTTCTGGCAATATCAAAACAATGAACGAGCCATTCACTCAATAATGATGAGTAATAAGGGCGATGATTTGCGCTCTGTGTTCTATGTTGATGGCGCTGCTTTCCCTGTTTTTGCGTTTATTGAAGATGGCCTGTCAATATCCGCACCTGGTGCTGATCTCGTTGTTAATGATACGACCTATAAGTTTGGGGCAACAAATCCGGCGACTGAATGTATCGCGGCGGACGTTATCCTTGATTTTAAGAGTGGGCGTGGTTTTTATGAGT  >  NC_000913/1430860‑1431128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A