Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I1 R1 120 93.5 3664254 88.3% 3235536 136.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,152,793 A→G R112R (CGT→CGC)  yegL ← IPR002035 domain‑containing protein YegL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,152,7930AG98.4% 220.7 / ‑4.4 62R112R (CGT→CGC) yegLIPR002035 domain‑containing protein YegL
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (25/36);  total (26/36)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

AAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAA  >  NC_000913/2152658‑2152927
                                                                                                                                       |                                                                                                                                      
aaagaaagCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTcgcg                                                                                                                                      >  1:944570/1‑138 (MQ=255)
 aagaaagCAAACCTTTTATCTTCTT‑CCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGtt                                                                                                                                    <  1:1641616/138‑1 (MQ=255)
   gaaagCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGttt                                                                                                                                   <  1:29263/138‑1 (MQ=255)
       gCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGc                                                                                                                               >  1:1540824/1‑138 (MQ=255)
         aaaCCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGctc                                                                                                                             <  1:828047/138‑1 (MQ=255)
         aaaCCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGctc                                                                                                                             <  2:694514/138‑1 (MQ=255)
         aaaCCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGctc                                                                                                                             >  2:431402/1‑138 (MQ=255)
              ttttATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTa                                                                                                                        <  2:12672/138‑1 (MQ=255)
                ttATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTAcc                                                                                                                      <  2:734742/138‑1 (MQ=255)
                ttATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTAcc                                                                                                                      >  2:644075/1‑138 (MQ=255)
                 tATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCa                                                                                                                     >  1:139254/1‑138 (MQ=255)
                         tcCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGctc                                                                                                                             <  2:1335987/122‑1 (MQ=255)
                         tcCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGctc                                                                                                                             >  1:1335987/1‑122 (MQ=255)
                         tcCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAgg                                                                                                             >  1:1395751/1‑138 (MQ=255)
                         tcCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAgg                                                                                                             >  1:936971/1‑138 (MQ=255)
                         tcCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAgg                                                                                                             >  1:341726/1‑138 (MQ=255)
                          ccccccGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAggg                                                                                                            <  2:1054907/138‑1 (MQ=255)
                          ccccccGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAggg                                                                                                            <  1:431402/138‑1 (MQ=255)
                               cGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTg                                                                                                       >  2:1572939/1‑138 (MQ=255)
                               cGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTg                                                                                                       >  1:993694/1‑138 (MQ=255)
                                 aaaCACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGt                                                                                                     <  2:1247486/138‑1 (MQ=255)
                                 aaaCACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGt                                                                                                     <  2:993694/138‑1 (MQ=255)
                                 aaaCACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGt                                                                                                     <  1:334207/138‑1 (MQ=255)
                                      cTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATgg                                                                                                <  2:1447452/138‑1 (MQ=255)
                                       tttGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGc                                                                                               <  1:1572939/138‑1 (MQ=255)
                                       tttGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGc                                                                                               <  1:1681748/138‑1 (MQ=255)
                                       tttGTTGGCTGCAGCCTGCCAATCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGAGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGc                                                                                               <  2:944570/138‑1 (MQ=255)
                                        ttgttgGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGcc                                                                                              <  2:706162/138‑1 (MQ=255)
                                        ttgttgGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGcc                                                                                              <  2:557558/138‑1 (MQ=255)
                                        ttgttgGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGcc                                                                                              <  2:841706/138‑1 (MQ=255)
                                         tgttgGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCg                                                                                             <  1:519734/138‑1 (MQ=255)
                                              gCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCAccc                                                                                        <  2:1345754/138‑1 (MQ=255)
                                                tGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCAt                                                                                      <  2:748379/138‑1 (MQ=255)
                                                tGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCAt                                                                                      <  2:936971/138‑1 (MQ=255)
                                                tGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCAt                                                                                      <  2:1395751/138‑1 (MQ=255)
                                                         ccACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATc                                                                             <  2:341726/138‑1 (MQ=255)
                                                               aTCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTg                                                                       <  2:259661/138‑1 (MQ=255)
                                                                 cGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTggg                                                                     >  2:1627787/1‑138 (MQ=255)
                                                                   gTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGgtg                                                                                 <  1:1776600/124‑1 (MQ=255)
                                                                   gTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGgtg                                                                                 >  2:1776600/1‑124 (MQ=255)
                                                                     tGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCaaa                                                                 >  1:1023790/1‑138 (MQ=255)
                                                                         gCACCATCGGTAATCAGGAAAATCCACGGGCGTTAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAgg                                                             >  2:1622915/1‑138 (MQ=255)
                                                                         gCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAgg                                                             >  2:1378474/1‑138 (MQ=255)
                                                                             cATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATgg                                                         >  2:760513/1‑138 (MQ=255)
                                                                                cGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCg                                                      >  2:1511575/1‑138 (MQ=255)
                                                                                  gTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGa                                                    <  2:1239229/138‑1 (MQ=255)
                                                                                  gTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGa                                                    <  2:1041191/138‑1 (MQ=255)
                                                                                    aaTCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGaaa                                                  >  1:1613951/1‑138 (MQ=255)
                                                                                            aaaTCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGGGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAg                                          >  2:16669/1‑138 (MQ=255)
                                                                                             aaTCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAgc                                         >  1:1214067/1‑138 (MQ=255)
                                                                                               tCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAgcgg                                       <  2:1214067/138‑1 (MQ=255)
                                                                                               tCCACGGGCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAgcgg                                       <  1:1627787/138‑1 (MQ=255)
                                                                                                     ggCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTgg                                 <  1:1511575/138‑1 (MQ=255)
                                                                                                     ggCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTgg                                 <  1:766742/138‑1 (MQ=255)
                                                                                                       cGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTa                               <  1:1114309/138‑1 (MQ=255)
                                                                                                           aataCGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAgg                           >  2:512032/1‑138 (MQ=255)
                                                                                                                    tACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCca                  >  1:376956/1‑138 (MQ=255)
                                                                                                                      ccATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCTGCGCTGGTAAAGGGTTGTTCcact                >  1:1311064/1‑137 (MQ=255)
                                                                                                                      ccATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCcaca                >  2:746457/1‑138 (MQ=255)
                                                                                                                          ttGCACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCa            >  1:1689386/1‑138 (MQ=255)
                                                                                                                             cACGATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCg         >  1:1215629/1‑138 (MQ=255)
                                                                                                                                gATACTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGc      <  1:760513/138‑1 (MQ=255)
                                                                                                                                    cTCGCGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGaa  <  1:1622915/138‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
AAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAA  >  NC_000913/2152658‑2152927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A