Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	120	93.5	3664254	88.3%	3235536	136.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,178,107	A→G	V153A (GTG→GCG)	fbaB ←	fructose‑bisphosphate aldolase class I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,178,107	0	A	G	97.7%	310.6 / ‑2.6	87	V153A (GTG→GCG)	fbaB	fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (42/43); total (43/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAGCACGCCGTAAGTTGACGCCAC  >  NC_000913/2177976‑2178240

cTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTgg                                                                                                                                 >  1:149495/1‑138 (MQ=255)

  gTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCa                                                                                                                               <  2:657716/138‑1 (MQ=255)

  gTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCa                                                                                                                               >  2:897680/1‑138 (MQ=255)

  gTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCa                                                                                                                               <  2:674285/138‑1 (MQ=255)

   tCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCAt                                                                                                                              >  1:1542015/1‑138 (MQ=255)

    cACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATa                                                                                                                             <  1:833800/138‑1 (MQ=255)

     aCCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATAc                                                                                                                            <  1:1139708/138‑1 (MQ=255)

        aTACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCa                                                                                                                               <  2:1365099/132‑1 (MQ=255)

        aTACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCa                                                                                                                               >  1:1365099/1‑132 (MQ=255)

        aTACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGc                                                                                                                         <  2:1412251/138‑1 (MQ=255)

          aCCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGt                                                                                                                       >  2:1114095/1‑138 (MQ=255)

          aCCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGt                                                                                                                       <  2:516048/138‑1 (MQ=255)

            ccAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGttt                                                                                                                     >  1:1580292/1‑138 (MQ=255)

             cAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTg                                                                                                                    <  2:102179/138‑1 (MQ=255)

             cAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTg                                                                                                                    >  1:1782733/1‑138 (MQ=255)

               gCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGAt                                                                                                                  >  2:958745/1‑138 (MQ=255)

               gCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGAt                                                                                                                  >  1:372311/1‑138 (MQ=255)

               gCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCAAGTTGAACGCCTGCTCCGCGCTGGCa                                                                                                                               <  1:1408829/125‑1 (MQ=255)

               gCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCAAGTTGAACGCCTGCTCCGCGCTGGCa                                                                                                                               >  2:1408829/1‑125 (MQ=255)

                  cGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGt                                                                                                               >  1:1556677/1‑138 (MQ=255)

                   gTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGt                                                                                                                       <  2:1352040/129‑1 (MQ=255)

                   gTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGt                                                                                                                       >  1:1352040/1‑129 (MQ=255)

                    tGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAg                                                                                                             <  1:1644570/138‑1 (MQ=255)

                      cgcACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGt                                                                                                           >  2:1029789/1‑138 (MQ=255)

                      cgcACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGt                                                                                                           <  1:1802648/138‑1 (MQ=255)

                      cgcACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGt                                                                                                           >  1:303524/1‑138 (MQ=255)

                      cgcACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGt                                                                                                           >  2:1413433/1‑138 (MQ=255)

                       gcACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTa                                                                                                          >  2:677980/1‑138 (MQ=255)

                          cGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTc                                                                                                       <  2:149495/138‑1 (MQ=255)

                            ttCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCgg                                                                                                     <  1:1731691/138‑1 (MQ=255)

                             ccAAAAGCCGCAGAAATTTCTTCAATCTGGCCACGTGACCCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCggg                                                                                                    <  2:1580292/137‑1 (MQ=255)

                               aaaaGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTa                                                                                                  <  1:594362/138‑1 (MQ=255)

                                  aGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACt                                                                                               <  2:1542015/138‑1 (MQ=255)

                                  aGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACt                                                                                               <  2:1556677/138‑1 (MQ=255)

                                       cAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCg                                                                                          >  2:893304/1‑138 (MQ=255)

                                        aGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGt                                                                                         <  2:1613494/138‑1 (MQ=255)

                                         gAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTctg                                                                                                     >  1:1245482/1‑123 (MQ=255)

                                         gAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTctg                                                                                                     <  2:1245482/125‑3 (MQ=255)

                                         gAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGtc                                                                                        >  2:1352290/1‑138 (MQ=255)

                                         gAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGtc                                                                                        >  2:1315478/1‑138 (MQ=255)

                                         gAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGtc                                                                                        >  2:606253/1‑138 (MQ=255)

                                         gAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGtc                                                                                        >  2:1073099/1‑138 (MQ=255)

                                         gAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGtc                                                                                        >  1:516332/1‑138 (MQ=255)

                                         gAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTGGATCGTAGGTATTCGGGTAACTTAGCGtc                                                                                        >  2:1281676/1‑138 (MQ=255)

                                          aaaTTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGtct                                                                                       <  1:1073099/138‑1 (MQ=255)

                                                cttcAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTtgt                                                                                 <  1:893304/138‑1 (MQ=255)

                                                cttcAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTtgt                                                                                 <  1:900967/138‑1 (MQ=255)

                                                  tcAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGa                                                                               <  1:606253/138‑1 (MQ=255)

                                                           cGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTg                                                                      <  1:472123/138‑1 (MQ=255)

                                                                tGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAg                                                                 <  2:474151/138‑1 (MQ=255)

                                                                tGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAg                                                                 <  1:1029789/138‑1 (MQ=255)

                                                                 gACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAgg                                                                >  1:62715/1‑138 (MQ=255)

                                                                  aCTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAgga                                                               <  1:885781/138‑1 (MQ=255)

                                                                       tCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAggaatgga                                                          <  1:81577/138‑1 (MQ=255)

                                                                       tCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAggaatgga                                                          <  2:372311/138‑1 (MQ=255)

                                                                       tCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAggaatgga                                                          <  1:677980/138‑1 (MQ=255)

                                                                            gCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAatgcg                                                     <  2:1782733/138‑1 (MQ=255)

                                                                             ccAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGgaatggaatg                                                       >  2:1202644/1‑135 (MQ=255)

                                                                             ccAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGgaatggaatg                                                       <  1:1202644/135‑1 (MQ=255)

                                                                             ccAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAatgcga                                                    >  2:710176/1‑138 (MQ=255)

                                                                                aaaTAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAatgcgatgc                                                 >  2:779048/1‑138 (MQ=255)

                                                                                 aaTAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAatgcgatgcg                                                <  1:710176/138‑1 (MQ=255)

                                                                                           cGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAAcgccg                                      <  1:211875/138‑1 (MQ=255)

                                                                                            gCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAAcgccgc                                     >  2:272232/1‑138 (MQ=255)

                                                                                            gCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAAcgccgc                                     >  2:18783/1‑138 (MQ=255)

                                                                                            gCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAAcgccgc                                     >  2:1776785/1‑138 (MQ=255)

                                                                                               ccAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGAt                                  >  2:1676345/1‑138 (MQ=255)

                                                                                                caaccgcaaccgCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATa                                            >  2:253268/1‑127 (MQ=255)

                                                                                                caaccgcaaccgCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATa                                            <  1:253268/127‑1 (MQ=255)

                                                                                                caaccgcaaccgCGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATa                                 >  1:1509075/1‑138 (MQ=255)

                                                                                                    cgcaaccgcGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGa                             >  2:1588911/1‑138 (MQ=255)

                                                                                                         ccgcGCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCa                        <  1:757470/138‑1 (MQ=255)

                                                                                                          cgcgCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAg                       >  1:884408/1‑138 (MQ=255)

                                                                                                          cgcgCCCATGTTGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAg                       >  2:871434/1‑138 (MQ=255)

                                                                                                                    ttGAACGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAGCACGCCGTaa             <  1:1776785/138‑1 (MQ=255)

                                                                                                                         cGCCTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAGCACGCCGTAAGTTGa        <  2:303524/138‑1 (MQ=255)

                                                                                                                            cTGCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAGCACGCCGTAAGTTGACGc     <  2:62715/138‑1 (MQ=255)

                                                                                                                              gCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAGCACGCCGTAAGTTGACGCca   <  2:581572/138‑1 (MQ=255)

                                                                                                                              gCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAGCACGCCGTAAGTTGACGCca   >  1:1689390/1‑138 (MQ=255)

                                                                                                                              gCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAGCACGCCGTAAGTTGACGCca   <  1:305432/138‑1 (MQ=255)

                                                                                                                              gCTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAGCACGCCGTAAGTTGACGCca   <  1:691964/138‑1 (MQ=255)

                                                                                                                              acTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAGCACGCCGTAAGTTGACGCca   >  2:556883/2‑138 (MQ=255)

                                                                                                                               cTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAGCACGCCGTAAGTTGACGCcac  >  1:581786/1‑138 (MQ=255)

                                                                                                                               cTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAGCACGCCGTAAGTTGACGCcac  >  2:411425/1‑138 (MQ=255)

                                                                                                                               cTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAGCACGCCGTAAGTTGACGCcac  >  1:1289115/1‑138 (MQ=255)

                                                                                                                               cTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAGCACGCCGTAAGTTGACGCcac  >  2:903383/1‑138 (MQ=255)

                                                                                                                               cTCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAGCACGCCGTAAGTTGACGCcac  >  2:302559/1‑138 (MQ=255)

                                                                                                                                tCCGCGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAGCACGCCGTaa             <  1:1758988/126‑1 (MQ=255)

CTGTCACCATACCCAGCTCGTGCGCACGTTCAAAAGCCGCAGAAATTTCTTCAATCTGGCGACGTGACTCTTCCGAGCCAAAATAGATAGTCGCACCAACCGCAACCGCGCCCATGTTGAACGCCTGCTCCACGCTGGCATACAGCGTTTGATCGTAGGTATTCGGGTAACTTAGCGTCTCGTTGTGATTAAGTTTGACGAGGAATGGAATGCGATGCGCATAACGCCGCGATACCGACGCCAGCACGCCGTAAGTTGACGCCAC  >  NC_000913/2177976‑2178240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A