Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F28 I1 R1
|
120 |
93.5 |
3664254 |
88.3% |
3235536 |
136.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,429,280 |
G→A |
P320L (CCG→CTG) |
purF ← |
amidophosphoribosyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,429,280 | 0 | G | A | 96.5%
| 185.2
/ ‑2.5
| 57 | P320L (CCG→CTG) | purF | amidophosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (29/26); total (30/27) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
TTTATCGCGGAACTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACA > NC_000913/2429156‑2429411
|
tttATCGCGGAACTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAAt < 2:190812/138‑1 (MQ=255)
aTCGCGGAACTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACg > 1:273424/1‑138 (MQ=255)
tCGCGGAACTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGa > 1:52706/1‑138 (MQ=255)
tCGCGGAACTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGa < 1:761301/138‑1 (MQ=255)
cgcgGAACTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAg < 2:613113/138‑1 (MQ=255)
gcgGAACTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGc > 2:343136/1‑138 (MQ=255)
ggCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAg < 2:1094209/138‑1 (MQ=255)
gcgGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAgcgc > 2:292791/1‑138 (MQ=255)
gcgGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAgcgc < 2:1516876/138‑1 (MQ=255)
cgGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGcgcg < 2:1004854/138‑1 (MQ=255)
gTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGat < 2:273424/138‑1 (MQ=255)
gCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATca < 1:77187/138‑1 (MQ=255)
gCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATca < 1:709950/138‑1 (MQ=255)
aTTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATcaca < 2:52706/138‑1 (MQ=255)
aTTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATcaca < 2:1021405/138‑1 (MQ=255)
tttACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAggg > 2:443573/1‑137 (MQ=255)
tACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGttt > 1:677741/1‑138 (MQ=255)
ggCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGat < 2:1092901/121‑1 (MQ=255)
ggCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGat > 1:1092901/1‑121 (MQ=255)
ggCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTg > 2:1713423/1‑138 (MQ=255)
cgcACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGtt > 2:1340697/1‑132 (MQ=255)
cgcACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGtt < 1:1340697/132‑1 (MQ=255)
cgcACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTggg < 2:1669177/138‑1 (MQ=255)
aCGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTgggatc > 2:364442/1‑138 (MQ=255)
cGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTgggatcg > 1:1334068/1‑138 (MQ=255)
ggACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTgggatcgg > 2:256809/1‑138 (MQ=255)
gACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTgggatcggg < 1:462545/138‑1 (MQ=255)
acgCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATcaccac < 1:1519802/138‑1 (MQ=255)
cgCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACg < 2:1334068/138‑1 (MQ=255)
gCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATTGGGATCACCACGt < 2:536117/138‑1 (MQ=255)
gctgctGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGa < 2:215296/138‑1 (MQ=255)
gctgctGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGa > 2:828312/1‑138 (MQ=255)
gctgctGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGa > 2:152668/1‑138 (MQ=255)
gctgctGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATACCACACGAGGTTTCTGGGATCGGGATCACCACGTCGa > 1:501576/1‑138 (MQ=255)
ctgctgGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGat > 2:1198548/1‑138 (MQ=255)
gctgGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCATGTCGatat > 1:897274/1‑138 (MQ=255)
gctgGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGGACAGTTTCCCCGGAATACGGGCAATTTCCAGCGCGATATCCCACGAGGTTTCTGGGATCGGGATCACCACGCCggaat > 2:681067/1‑134 (MQ=255)
tgGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATcc < 1:1821953/138‑1 (MQ=255)
cccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGt > 1:1119252/1‑127 (MQ=255)
cccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGt < 2:1119252/127‑1 (MQ=255)
aaaGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTc < 2:677741/138‑1 (MQ=255)
aaaGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTc < 2:1801928/138‑1 (MQ=255)
aaaGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTc > 1:1199237/1‑138 (MQ=255)
gCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATAAGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGc > 2:629211/1‑138 (MQ=255)
ggCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCaa > 2:485782/1‑138 (MQ=255)
ggCCAACATAACGGTTTTTAACGGAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCaa < 1:681067/138‑1 (MQ=255)
gCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAAt > 1:576209/1‑138 (MQ=255)
ccAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAAtt > 2:1329666/1‑138 (MQ=255)
ccAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAAtt > 1:780501/1‑138 (MQ=255)
cAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAAttt > 1:182971/1‑138 (MQ=255)
cATAGCGGTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTtct > 2:951778/1‑138 (MQ=255)
gTTTTTAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAg > 2:1058306/1‑138 (MQ=255)
tttAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCATGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGttt < 2:897274/138‑1 (MQ=255)
tttAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGttt > 1:225781/1‑138 (MQ=255)
tttAACGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGttt < 2:182971/138‑1 (MQ=255)
aaCGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGt > 1:1335150/1‑138 (MQ=255)
aaCGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGt > 1:664366/1‑138 (MQ=255)
aCGAAGCCCTGGCGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTg < 1:1198548/138‑1 (MQ=255)
cGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGc < 1:629211/138‑1 (MQ=255)
cGGTACAGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTcaca < 1:1646352/138‑1 (MQ=255)
|
TTTATCGCGGAACTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACA > NC_000913/2429156‑2429411
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
