Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	120	93.5	3664254	88.3%	3235536	136.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,941,643	A→G	intergenic (‑12/+7)	ygdD ← / ← gcvA	conserved inner membrane protein YgdD/DNA‑binding transcriptional dual regulator GcvA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,941,643	0	A	G	100.0%	154.4 / NA	44	intergenic (‑12/+7)	ygdD/gcvA	conserved inner membrane protein YgdD/DNA‑binding transcriptional dual regulator GcvA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/17); total (27/17)

CTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTT  >  NC_000913/2941524‑2941777

ctcAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATa                                                                                                                      >  1:481603/1‑138 (MQ=255)

ctcAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATa                                                                                                                      >  1:285498/1‑138 (MQ=255)

 tcAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATaa                                                                                                                     >  1:1002983/1‑138 (MQ=255)

  cAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAAc                                                                                                                    >  2:366574/1‑138 (MQ=255)

          cccATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCgg                                                                                                            >  2:348792/1‑138 (MQ=255)

            cATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGaa                                                                                                          >  2:155078/1‑138 (MQ=255)

            cATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGaa                                                                                                          >  1:1385635/1‑138 (MQ=255)

                       ttAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGtt                                                                                               >  1:512433/1‑138 (MQ=255)

                            acaTGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTcagca                                                                                          >  1:712789/1‑138 (MQ=255)

                            acaTGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTcagca                                                                                          >  2:726697/1‑138 (MQ=255)

                            acaTGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTcagca                                                                                          >  1:1201349/1‑138 (MQ=255)

                             caTGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTcagcag                                                                                         >  1:1092757/1‑138 (MQ=255)

                                gcgcgcCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCgg                                                                                      <  2:1277626/138‑1 (MQ=255)

                                gcgcgcCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCgg                                                                                      <  2:1002983/138‑1 (MQ=255)

                                gcgcgcCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCgg                                                                                      >  1:1324315/1‑138 (MQ=255)

                                  gcgcCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCt                                                                                    >  1:1521762/1‑138 (MQ=255)

                                  gcgcCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCt                                                                                    >  1:1744908/1‑138 (MQ=255)

                                  gcgcCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCt                                                                                    >  2:680067/1‑138 (MQ=255)

                                  gcgcCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCt                                                                                    <  1:155078/138‑1 (MQ=255)

                                     ccAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTc                                                                                 >  1:488281/1‑138 (MQ=255)

                                     ccAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTc                                                                                 >  1:1210930/1‑138 (MQ=255)

                                     ccAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTc                                                                                 <  2:1385635/138‑1 (MQ=255)

                                              cccAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATc                                                                        <  1:348792/138‑1 (MQ=255)

                                              cccAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATc                                                                        <  2:488281/138‑1 (MQ=255)

                                                       acaAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGa                                                               >  2:1543102/1‑138 (MQ=255)

                                                            aaTGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAggc                                                          <  1:680067/138‑1 (MQ=255)

                                                            aaTGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAggc                                                          <  2:481603/138‑1 (MQ=255)

                                                              tGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAggcgg                                                        <  2:1092757/138‑1 (MQ=255)

                                                                      cTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTa                                                <  2:1521762/138‑1 (MQ=255)

                                                                      cTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTa                                                <  2:512433/138‑1 (MQ=255)

                                                                      cTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTa                                                <  1:1778605/138‑1 (MQ=255)

                                                                        aaTGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTAcc                                              >  2:512299/1‑138 (MQ=255)

                                                                               gcgAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCt                                       >  1:1818114/1‑138 (MQ=255)

                                                                                 gAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGc                                     <  2:712789/138‑1 (MQ=255)

                                                                                  aaaaTCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGcc                                    <  2:1324315/138‑1 (MQ=255)

                                                                                   aaaTCAGCATAAAACGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCt                                   <  1:726060/138‑1 (MQ=255)

                                                                                                 cGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACaa                     >  2:1296373/1‑138 (MQ=255)

                                                                                                 cGGCTGGTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACaa                     <  1:1543102/138‑1 (MQ=255)

                                                                                                      ggTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAg                >  1:1309706/1‑138 (MQ=255)

                                                                                                      ggTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAg                >  2:1003973/1‑138 (MQ=255)

                                                                                                      ggTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAg                >  1:700385/1‑138 (MQ=255)

                                                                                                       gTCATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGa               >  1:655350/1‑138 (MQ=255)

                                                                                                         cATGGTCGTACCCTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATa             >  2:216916/1‑138 (MQ=255)

                                                                                                                    ccTGCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCAtttt  <  1:1498632/138‑1 (MQ=255)

CTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTT  >  NC_000913/2941524‑2941777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A