Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 180 66.5 2263572 99.4% 2249990 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,941,730 Δ1 bp coding (838/918 nt) gcvA ← DNA‑binding transcriptional dual regulator GcvA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,941,7270T.100.0% 169.6 / NA 40coding (841/918 nt)gcvADNA‑binding transcriptional dual regulator GcvA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (22/18);  total (22/18)

AAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCA  >  NC_000913/2941588‑2941861
                                                                                                                                           |                                                                                                                                      
aaGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTAt                                                                                                                                        <  1:769958/140‑1 (MQ=255)
 aGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTAtt                                                                                                                                       >  1:1109550/1‑140 (MQ=255)
 aGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTAtt                                                                                                                                       >  1:176196/1‑140 (MQ=255)
    cGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTAtttac                                                                                                                                    <  2:176196/140‑3 (MQ=255)
    cGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTAtttac                                                                                                                                    <  2:1109550/140‑3 (MQ=255)
    cGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTAtttac                                                                                                                                    <  2:400059/140‑3 (MQ=255)
       tAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCa                                                                                                                                >  1:80414/1‑140 (MQ=255)
         aTGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTAt                                                                                                                                        >  1:752970/1‑131 (MQ=255)
         aTGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTAt                                                                                                                                        <  2:752970/131‑1 (MQ=255)
                cgAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTACCt                                                                                                                       >  2:550720/1‑140 (MQ=255)
                 gAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTg                                                                                                                      >  2:1050729/1‑140 (MQ=255)
                 gAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTg                                                                                                                      <  1:345784/140‑1 (MQ=255)
                      tCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGt                                                                                                                 <  1:467422/140‑1 (MQ=255)
                      tCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGt                                                                                                                 <  1:8243/140‑1 (MQ=255)
                               aaCGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACaaa                                                                                                        >  1:184881/1‑140 (MQ=255)
                                       gTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATaa                                                                                                >  1:228253/1‑140 (MQ=255)
                                         cATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTACCTGACTGTCATGACAAACCAGATaaaa                                                                                              <  1:550720/140‑1 (MQ=255)
                                                 tACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCAtttt                                                                                      >  2:282662/1‑140 (MQ=255)
                                                  aCCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCAttttt                                                                                     >  2:752706/1‑140 (MQ=255)
                                                  aCCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCAttttt                                                                                     >  1:949441/1‑140 (MQ=255)
                                                  aCCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCG‑CAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTa                                                                                    <  2:1076031/140‑1 (MQ=255)
                                                   cccTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTa                                                                                    <  1:67303/140‑1 (MQ=255)
                                                       aCGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGa                                                                                >  1:765119/1‑140 (MQ=255)
                                                        cGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTa                                                                                    >  1:281046/1‑135 (MQ=255)
                                                        cGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTa                                                                                    <  2:281046/135‑1 (MQ=255)
                                                        cGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGAc                                                                               >  1:433994/1‑140 (MQ=255)
                                                            aaTTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGa                                                                           <  1:644731/140‑1 (MQ=255)
                                                              ttattGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAAc                                                                         >  1:547237/1‑140 (MQ=255)
                                                                    ttCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCAtt                                                                   >  1:943961/1‑140 (MQ=255)
                                                                    ttCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCAtt                                                                   >  1:931370/1‑140 (MQ=255)
                                                                          aCGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCACTAAACgg                                                             <  2:228253/140‑1 (MQ=255)
                                                                           cGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACggg                                                            >  1:962363/1‑140 (MQ=255)
                                                                                 cGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACa                                                      >  1:502840/1‑140 (MQ=255)
                                                                                   gAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAg                                                    >  2:335328/1‑140 (MQ=255)
                                                                                             tGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGcc                                          <  1:1050729/140‑1 (MQ=255)
                                                                                             tGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATCTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGcc                                          <  2:440494/140‑1 (MQ=255)
                                                                                                    cagcGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGAttt                                   <  2:765119/140‑1 (MQ=255)
                                                                                                                 cAGGATCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCAt                      <  2:80414/140‑1 (MQ=255)
                                                                                                                    gctCCATTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTCCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCAc                   >  2:821208/3‑140 (MQ=255)
                                                                                                                         aTTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGAt                              >  2:253530/1‑124 (MQ=255)
                                                                                                                         aTTGGCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGAt                              <  1:253530/124‑1 (MQ=255)
                                                                                                                             gCGAAAGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGc          <  2:921521/140‑1 (MQ=255)
                                                                                                                                aaaGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAg       >  2:726466/1‑140 (MQ=255)
                                                                                                                                aaaGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAg       >  1:474090/1‑140 (MQ=255)
                                                                                                                                  aGGCGGCTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAgcg     >  2:215530/1‑140 (MQ=255)
                                                                                                                                   ggcggcTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAgcgc    <  2:502840/140‑1 (MQ=255)
                                                                                                                                   ggcggcTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAgcgc    <  2:962363/140‑1 (MQ=255)
                                                                                                                                   ggcggcTA‑TTTACCCAGTTCTGCCTGACTGCCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAgcgc    <  2:332852/140‑1 (MQ=255)
                                                                                                                                     cggcTA‑TTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCa  <  2:310709/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                      
AAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGGTCGTACCCTACGTAAATTATTGTTCATAACGAAAGCGGAATTTTTCTTGTTCAGCAGCGGCTTTCGCCAGGATCCATTGGCGAAAGGCGGCTATTTTACCCAGTTCTGCCTGACTGTCATGACAAACCAGATAAAAAGCATTTTTACTGACCAGAACATCATTAAACGGGCAAACAAGACGTCCGGCCTCGATTTCAGATTGCGCCATCACGTTATTTGCCAGCGCCA  >  NC_000913/2941588‑2941861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A