Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	120	93.5	3664254	88.3%	3235536	136.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,071,327	A→G	intergenic (‑83/+132)	fbaA ← / ← pgk	fructose‑bisphosphate aldolase class II/phosphoglycerate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,071,327	0	A	G	98.5%	243.4 / ‑4.8	68	intergenic (‑83/+132)	fbaA/pgk	fructose‑bisphosphate aldolase class II/phosphoglycerate kinase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (33/34); total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CGTCATCACCAGTGATTACGCCAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTT  >  NC_000913/3071193‑3071458

cGTCATCACCAGTGATTACGCCAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCgctt                                                                                                                                  <  2:1779941/138‑1 (MQ=255)

       aCCAGTGATTACGCCAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATg                                                                                                                           <  2:1700428/138‑1 (MQ=255)

       aCCAGTGATTACGCCAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATg                                                                                                                           <  1:1679942/138‑1 (MQ=255)

        ccAGTGATTACGCCAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGt                                                                                                                          >  2:838861/1‑138 (MQ=255)

          aGTGATTACGCCAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCCGTCGCTTACTTATGTaa                                                                                                                        >  2:1538651/1‑138 (MQ=255)

             gATTACGCCAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCt                                                                                                                     >  2:1759845/1‑138 (MQ=255)

             gATTACGCCAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTCATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCt                                                                                                                     >  1:1708036/1‑138 (MQ=255)

              aTTACGCCAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTc                                                                                                                    >  1:1799242/1‑138 (MQ=255)

              aTTACGCCAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTc                                                                                                                    >  2:1161215/1‑138 (MQ=255)

              aTTACGCCAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTc                                                                                                                    <  2:1233487/138‑1 (MQ=255)

                tACGCCAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAg                                                                                                                       >  2:864863/1‑133 (MQ=255)

                tACGCCAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAg                                                                                                                       <  1:864863/133‑1 (MQ=255)

                 aCGCCAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTg                                                                                                                 <  2:59785/138‑1 (MQ=255)

                   gCCAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATg                                                                                                                           <  1:546397/126‑1 (MQ=255)

                   gCCAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATg                                                                                                                           >  2:546397/1‑126 (MQ=255)

                     cAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGa                                                                                                             >  1:1387019/1‑138 (MQ=255)

                     cAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGa                                                                                                             >  1:854580/1‑138 (MQ=255)

                     cAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGa                                                                                                             >  1:1720445/1‑138 (MQ=255)

                        gTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTcc                                                                                                                   >  2:875467/1‑129 (MQ=255)

                        gTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTcc                                                                                                                   <  1:875467/129‑1 (MQ=255)

                         ttttACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCa                                                                                                         >  1:232016/1‑138 (MQ=255)

                         ttttACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGCTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCa                                                                                                         >  2:1508164/1‑138 (MQ=255)

                                  aTCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGt                                                                                                <  1:1759845/138‑1 (MQ=255)

                                    cAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGt                                                                                                >  2:1713701/1‑136 (MQ=255)

                                    cAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGt                                                                                                <  1:1713701/136‑1 (MQ=255)

                                    cAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCg                                                                                              >  1:1126631/1‑138 (MQ=255)

                                              aGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCa                                                                                    <  2:576854/138‑1 (MQ=255)

                                              aGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCa                                                                                    <  2:931737/138‑1 (MQ=255)

                                               gACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCaa                                                                                   >  2:646897/1‑138 (MQ=255)

                                               gACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCaa                                                                                   >  1:574983/1‑138 (MQ=255)

                                                aCATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAAc                                                                                  <  1:1087319/138‑1 (MQ=255)

                                                aCATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAAc                                                                                  <  2:1799242/138‑1 (MQ=255)

                                                  aTGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTc                                                                                <  2:574983/138‑1 (MQ=255)

                                                      ctctTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCg                                                                               >  2:612360/1‑135 (MQ=255)

                                                      ctctTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCg                                                                               <  1:612360/135‑1 (MQ=255)

                                                        ctTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAatta                                                                          <  2:854580/138‑1 (MQ=255)

                                                        ctTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAatta                                                                          <  2:487155/138‑1 (MQ=255)

                                                        ctTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAatta                                                                          <  1:1679544/138‑1 (MQ=255)

                                                            tCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTa                                                                      >  1:1813897/1‑138 (MQ=255)

                                                              cTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTaga                                                                    <  2:1813897/138‑1 (MQ=255)

                                                                  aTCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTAt                                                                >  1:194757/1‑138 (MQ=255)

                                                                     gtcgGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGaa                                                             >  2:1096884/1‑138 (MQ=255)

                                                                        gggCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGaaaaa                                                          >  2:481625/1‑138 (MQ=255)

                                                                           ccTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAattatt                                                                        <  1:961205/121‑1 (MQ=25)

                                                                           ccTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAattatt                                                                        >  2:961205/1‑121 (MQ=25)

                                                                           ccTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTg                                                       <  2:232016/138‑1 (MQ=255)

                                                                           ccTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTg                                                       <  2:1238542/138‑1 (MQ=255)

                                                                           ccTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTg                                                       <  2:194757/138‑1 (MQ=255)

                                                                           ccTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTg                                                       <  1:23342/138‑1 (MQ=255)

                                                                           ccTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTg                                                       <  1:481625/138‑1 (MQ=255)

                                                                           ccTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTg                                                       <  1:646897/138‑1 (MQ=255)

                                                                           ccTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTg                                                       <  2:1126631/138‑1 (MQ=255)

                                                                           ccTTATACTCGTCATACTTCAAGCTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTg                                                       <  1:1508164/138‑1 (MQ=255)

                                                                           ccTCATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTg                                                       <  2:1708036/138‑1 (MQ=255)

                                                                             ttATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCg                                                     >  2:630441/1‑138 (MQ=255)

                                                                             ttATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCg                                                     >  2:726784/1‑138 (MQ=255)

                                                                                      tCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTaaa                                            >  1:1152196/1‑138 (MQ=255)

                                                                                        aTACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAg                                          >  2:244763/1‑138 (MQ=255)

                                                                                          aCTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAgcg                                        >  1:803928/1‑138 (MQ=255)

                                                                                                 gTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGaa                                 <  2:803928/138‑1 (MQ=255)

                                                                                                      aTGTGCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAg                            >  2:1423899/1‑138 (MQ=255)

                                                                                                      aTGGGCTACGTCTGAGTTCGCTCGCCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAg                            >  2:40932/1‑138 (MQ=255)

                                                                                                        gtgCTGCGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAggg                          >  2:870138/1‑138 (MQ=255)

                                                                                                         tgctgcGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCT‑AAAGCGCGCTGAAACAAGGGCa                        <  1:1646503/138‑1 (MQ=255)

                                                                                                          gctgcGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCa                        <  1:870138/138‑1 (MQ=255)

                                                                                                          gctgcGTCTGCGTTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCa                        <  2:1387019/138‑1 (MQ=255)

                                                                                                                tctgcgtTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTc                  <  2:529956/138‑1 (MQ=255)

                                                                                                                tctgcgtTCGCTCACCCCAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTc                  >  1:1074639/1‑138 (MQ=255)

                                                                                                                         gCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTccctgccctg         <  1:40932/138‑1 (MQ=255)

                                                                                                                                ccAGTCGCTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGAtttt  >  1:105350/1‑138 (MQ=255)

CGTCATCACCAGTGATTACGCCAGGTTTTACGAAATCAAAAATCTTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTT  >  NC_000913/3071193‑3071458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A