Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	120	93.5	3664254	88.3%	3235536	136.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,729,268	C→T	R35H (CGC→CAC)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,729,268	0	C	T	99.2%	425.8 / ‑4.8	124	R35H (CGC→CAC)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (66/57); total (66/58)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 126 total aligned reads displayed.

TCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTAT  >  NC_000913/3729135‑3729402

tCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGa                                                                                                                                    >  1:1225553/1‑138 (MQ=255)

 ccGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGaa                                                                                                                                   >  2:630850/1‑138 (MQ=255)

    tGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACg                                                                                                                                <  2:92469/138‑1 (MQ=255)

      cATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGt                                                                                                                              >  1:959150/1‑138 (MQ=255)

      cATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGt                                                                                                                              <  2:1159718/138‑1 (MQ=255)

      cATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGt                                                                                                                              >  2:3833/1‑138 (MQ=255)

                        cAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTgcgc                                                                                                            <  2:787320/138‑1 (MQ=255)

                        cAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTgcgc                                                                                                            >  1:1017790/1‑138 (MQ=255)

                          aTGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCgcag                                                                                                          <  2:15516/138‑1 (MQ=255)

                                 aaCGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCgca                                                                                                           <  2:842391/130‑1 (MQ=255)

                                 aaCGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCgca                                                                                                           >  1:842391/1‑130 (MQ=255)

                                  aCGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAaccacc                                                                                                  >  2:1651835/1‑138 (MQ=255)

                                       cTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCAcc                                                                                             >  2:1239506/1‑138 (MQ=255)

                                        tGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCAccc                                                                                            <  2:1168980/138‑1 (MQ=255)

                                         gCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAacca                                                                                                    <  2:394510/129‑1 (MQ=255)

                                         gCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAacca                                                                                                    >  1:394510/1‑129 (MQ=255)

                                         gCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCt                                                                                           >  1:591670/1‑138 (MQ=255)

                                                 tGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGTGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTg                                                                                   >  2:1750134/1‑138 (MQ=255)

                                                 tGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTg                                                                                   <  1:1651835/138‑1 (MQ=255)

                                                 tGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTg                                                                                   <  1:3833/138‑1 (MQ=255)

                                                   cTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAg                                                                                 >  1:647119/1‑138 (MQ=255)

                                                    tGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGc                                                                                >  1:1172140/1‑138 (MQ=255)

                                                      aTCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCaa                                                                              >  2:1544990/1‑138 (MQ=255)

                                                      aTCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCaa                                                                              >  2:322741/1‑138 (MQ=255)

                                                      aTCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCaa                                                                              >  1:205989/1‑138 (MQ=255)

                                                      aTCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCaa                                                                              >  2:1662510/1‑138 (MQ=255)

                                                       tCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCaaa                                                                             >  1:1521853/1‑138 (MQ=255)

                                                        cGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCaaaa                                                                            >  1:5651/1‑138 (MQ=255)

                                                         gCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAAt                                                                           >  1:943750/1‑138 (MQ=255)

                                                           ccAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGtt                                                                                    >  1:1334846/1‑127 (MQ=255)

                                                           ccAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAg                                                                                 >  1:1019155/1‑130 (MQ=255)

                                                           ccAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAg                                                                                 <  2:1019155/130‑1 (MQ=255)

                                                           ccAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATaa                                                                         >  1:900261/1‑138 (MQ=255)

                                                           ccAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATaa                                                                         >  1:1616170/1‑138 (MQ=255)

                                                             agagCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACt                                                                       >  2:588850/1‑138 (MQ=255)

                                                                gCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACtttt                                                                    >  1:200222/1‑138 (MQ=255)

                                                                     cATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGcc                                                               <  2:831210/138‑1 (MQ=255)

                                                                     cATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGcc                                                               <  1:1215668/138‑1 (MQ=255)

                                                                      aTTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGccc                                                              >  2:180435/1‑138 (MQ=255)

                                                                      aTTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGccc                                                              <  1:96931/138‑1 (MQ=255)

                                                                       ttGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCg                                                             <  2:1536689/138‑1 (MQ=255)

                                                                          cgcgATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAgg                                                          <  2:647119/138‑1 (MQ=255)

                                                                           gcgATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGt                                                         >  1:1523200/1‑138 (MQ=255)

                                                                            cgATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTg                                                        >  2:863626/1‑138 (MQ=255)

                                                                            cgATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTg                                                        >  2:709476/1‑138 (MQ=255)

                                                                             gATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGCCCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTg                                                        <  2:1618668/137‑1 (MQ=255)

                                                                             gATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTg                                                        >  1:1618668/1‑137 (MQ=255)

                                                                                cAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCaa                                                    >  2:1795708/1‑138 (MQ=255)

                                                                                cAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCaa                                                    >  1:1721460/1‑138 (MQ=255)

                                                                                 aGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAg                                                   >  2:1755374/1‑138 (MQ=255)

                                                                                 aGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAg                                                   >  2:1374959/1‑138 (MQ=255)

                                                                                     gcctgccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCt                                               <  2:900261/138‑1 (MQ=255)

                                                                                      cctgccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTa                                              <  1:180435/138‑1 (MQ=255)

                                                                                       ctgccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCCAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTAt                                             <  1:1609247/138‑1 (MQ=255)

                                                                                        tgccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATc                                            <  2:1200300/138‑1 (MQ=255)

                                                                                         gccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATcc                                           <  2:943750/138‑1 (MQ=255)

                                                                                         gccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATcc                                           <  2:1485581/138‑1 (MQ=255)

                                                                                         gccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATcc                                           <  1:588850/138‑1 (MQ=255)

                                                                                          ccaccaCTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATccc                                          <  1:1193899/138‑1 (MQ=255)

                                                                                           caccacTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCg                                         <  2:205989/138‑1 (MQ=255)

                                                                                           caccacTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCg                                         >  1:1390794/1‑138 (MQ=255)

                                                                                            accacTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTTAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGa                                        <  1:822887/138‑1 (MQ=255)

                                                                                            accacTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGa                                        <  2:959150/138‑1 (MQ=255)

                                                                                            accacTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGa                                        <  2:1807697/138‑1 (MQ=255)

                                                                                            accacTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGa                                        <  1:1611156/138‑1 (MQ=255)

                                                                                            accacTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGa                                        <  2:1721460/138‑1 (MQ=255)

                                                                                               acTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGatat                                     >  2:301590/1‑138 (MQ=255)

                                                                                               acTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGatat                                     >  1:1384237/1‑138 (MQ=255)

                                                                                                     ccGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGa                                        >  2:176775/1‑129 (MQ=255)

                                                                                                     ccGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGa                                        <  1:176775/129‑1 (MQ=255)

                                                                                                     ccGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACatat                               >  1:1238829/1‑138 (MQ=255)

                                                                                                     ccGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACatat                               >  1:508753/1‑138 (MQ=255)

                                                                                                      cGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATc                                                >  2:432259/1‑120 (MQ=255)

                                                                                                      cGGGTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATc                                                <  1:432259/120‑1 (MQ=255)

                                                                                                       gggTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTa                                              >  1:482581/1‑121 (MQ=255)

                                                                                                       gggTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTa                                              <  2:482581/121‑1 (MQ=255)

                                                                                                        ggTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGa                                        <  2:919613/126‑1 (MQ=255)

                                                                                                        ggTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGa                                        >  1:919613/1‑126 (MQ=255)

                                                                                                         gTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATatcgat                           <  2:959998/138‑1 (MQ=255)

                                                                                                         gTCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATatcgat                           <  2:1238829/138‑1 (MQ=255)

                                                                                                          tCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATatcgatc                          >  1:356896/1‑138 (MQ=255)

                                                                                                          tCTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGATTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCTCGATATACATatcgatc                          >  2:1630138/1‑138 (MQ=255)

                                                                                                           cTTGTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATatcgatcg                         <  2:1504391/138‑1 (MQ=255)

                                                                                                              gTTCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTc                      <  1:1662510/138‑1 (MQ=255)

                                                                                                               ttCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTcc                     >  2:1521422/1‑138 (MQ=255)

                                                                                                               ttCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTcc                     >  2:1159403/1‑138 (MQ=255)

                                                                                                               ttCCGACCAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTcc                     >  2:796763/1‑138 (MQ=255)

                                                                                                                      cAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTaaaaa              >  1:499733/1‑138 (MQ=255)

                                                                                                                      cAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTaaaaa              >  1:793189/1‑138 (MQ=255)

                                                                                                                      cAGAGTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTaaaaa              >  1:663257/1‑138 (MQ=255)

                                                                                                                          gTGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATg          <  2:1523200/138‑1 (MQ=255)

                                                                                                                           tGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGc         <  2:356896/138‑1 (MQ=255)

                                                                                                                           tGGATGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGc         >  1:263756/1‑138 (MQ=255)

                                                                                                                               tGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCgg     <  1:560162/138‑1 (MQ=255)

                                                                                                                               tGCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCgg     <  2:1017790/138‑1 (MQ=255)

                                                                                                                                gCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGt    >  1:666567/1‑138 (MQ=255)

                                                                                                                                gCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGt    <  1:709476/138‑1 (MQ=255)

                                                                                                                                gCGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAAATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGt    <  2:1172140/138‑1 (MQ=255)

                                                                                                                                 cGGGTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTa   >  1:860364/1‑138 (MQ=255)

                                                                                                                                  gggTGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTAt  <  2:1309906/138‑1 (MQ=255)

TCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTAT  >  NC_000913/3729135‑3729402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A