Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,729,603	G→A	S388F (TCC→TTC)	xylA ←	xylose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,729,603	0	G	A	100.0%	175.9 / NA	50	S388F (TCC→TTC)	xylA	xylose isomerase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/27); total (23/27)

CAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGA  >  NC_000913/3729470‑3729734

cAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAAc                                                                                                                               >  1:767168/1‑140 (MQ=255)

         cAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGa                                                                                                                      <  1:135244/140‑1 (MQ=255)

         cAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGa                                                                                                                      <  2:767168/140‑1 (MQ=255)

         cAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGa                                                                                                                      <  2:123188/140‑1 (MQ=255)

                          cACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCg                                                                                                     <  2:669506/140‑1 (MQ=255)

                          cACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCg                                                                                                     <  1:429060/140‑1 (MQ=255)

                               tGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATc                                                                                                >  1:630518/1‑140 (MQ=255)

                                    cACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGcc                                                                                                   <  1:434220/132‑1 (MQ=255)

                                    cACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGcc                                                                                                   >  2:434220/1‑132 (MQ=255)

                                    cACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGcc                                                                                                   <  1:515815/132‑1 (MQ=255)

                                    cACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGcc                                                                                                   >  2:515815/1‑132 (MQ=255)

                                       cGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCa                                                                                        >  1:265879/1‑140 (MQ=255)

                                       cGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCa                                                                                        >  1:475603/1‑140 (MQ=255)

                                       cGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCa                                                                                        >  1:248713/1‑140 (MQ=255)

                                       cGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCa                                                                                        >  2:247295/1‑140 (MQ=255)

                                         gagaCAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATg                                                                                      >  2:303930/1‑140 (MQ=255)

                                                   atgTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCaa                                                                            >  2:206958/1‑140 (MQ=255)

                                                               atatTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCa                                                                <  1:173014/140‑1 (MQ=255)

                                                                 atTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGt                                                              <  2:248713/140‑1 (MQ=255)

                                                                 atTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGt                                                              >  1:694193/1‑140 (MQ=255)

                                                                  tttGGCTAAATCTGCCAGTGACATTCGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTg                                                             <  2:519788/140‑1 (MQ=255)

                                                                      gCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCAt                                                         >  2:448983/1‑140 (MQ=255)

                                                                      gCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCAt                                                         <  1:303930/140‑1 (MQ=255)

                                                                       cTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATc                                                        >  2:132186/1‑140 (MQ=255)

                                                                        tAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCg                                                       >  2:674586/1‑140 (MQ=255)

                                                                              tGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCa                                                 <  1:444696/140‑1 (MQ=255)

                                                                                ccAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATc                                               <  2:265879/140‑1 (MQ=255)

                                                                                  aGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATcgc                                             <  2:448177/140‑1 (MQ=255)

                                                                                  aGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATcgc                                             <  2:630518/140‑1 (MQ=255)

                                                                                   gTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATcgcg                                            <  2:475603/140‑1 (MQ=255)

                                                                                   gTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATcgcg                                            <  1:132186/140‑1 (MQ=255)

                                                                                     gACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGcc                                          >  1:256523/1‑140 (MQ=255)

                                                                                     gACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGcc                                          >  1:16632/1‑140 (MQ=255)

                                                                                          ttGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGatat                                     <  1:698652/140‑1 (MQ=255)

                                                                                          ttGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGatat                                     <  1:769835/140‑1 (MQ=255)

                                                                                          ttGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGatat                                     <  1:4612/140‑1 (MQ=255)

                                                                                               cTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCGATCGCGCCGATATGACCg                                <  1:206958/140‑1 (MQ=255)

                                                                                                  tCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCTATCGTATCCATCGCGCCGATATGACCGTaa                             >  2:542722/1‑140 (MQ=255)

                                                                                                  tCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTaa                             >  1:486111/1‑140 (MQ=255)

                                                                                                    aGGATTTGCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTaaaa                           <  1:674586/140‑1 (MQ=255)

                                                                                                           gCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCa                    >  1:473662/1‑140 (MQ=255)

                                                                                                           gCTGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCa                    >  2:852294/1‑140 (MQ=255)

                                                                                                             tGGCCCAATTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCata                  <  2:256523/140‑1 (MQ=255)

                                                                                                                    aTTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCa           >  2:871963/1‑140 (MQ=255)

                                                                                                                    aTTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCa           <  2:13773/140‑1 (MQ=255)

                                                                                                                    aTTCGCTATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCa           >  1:92189/1‑140 (MQ=255)

                                                                                                                          tATTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACtt     >  2:521654/1‑140 (MQ=255)

                                                                                                                           aTTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACttt    <  1:871963/140‑1 (MQ=255)

                                                                                                                           aTTCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACttt    <  1:448983/140‑1 (MQ=255)

                                                                                                                             tCCAGCCGAAATAACGCTGCGCGATGCGTTTATCCAGCTCGTCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTga  <  1:388963/140‑1 (MQ=255)

CAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTACTTTGA  >  NC_000913/3729470‑3729734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A