Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I1 R1 120 93.5 3664254 88.3% 3235536 136.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,375,940 T→C F81S (TTC→TCC)  efp → protein chain elongation factor EF‑P

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,375,9400TC99.0% 361.7 / ‑4.9 98F81S (TTC→TCC) efpprotein chain elongation factor EF‑P
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (46/51);  total (47/51)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CATTTGCTCGCGTTAAACTGCGTCGTCTGCTGACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTTCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGCCGATCTCCGTT  >  NC_000913/4375808‑4376073
                                                                                                                                    |                                                                                                                                     
cATTTGCTCGCGTTAAACTGCGTCGTCTGCTGACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGc                                                                                                                                  <  1:51252/138‑1 (MQ=255)
cATTTGCTCGCGTTAAACTGCGTCGTCTGCTGACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGc                                                                                                                                  >  2:1576096/1‑138 (MQ=255)
 aTTTGCTCGCGTTAAACTGCGTCGTCTGCTGACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCa                                                                                                                                 >  2:1400515/1‑138 (MQ=255)
           gTTAAACTGCGTCGTCTGCTGACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGaac                                                                                                                       >  1:636055/1‑138 (MQ=255)
           gTTAAACTGCGTCGTCTGCTGACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGaac                                                                                                                       >  2:877578/1‑138 (MQ=255)
                aCTGCGTCGTCTGCTGACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGa                                                                                                                  <  1:1576096/138‑1 (MQ=255)
                      tcgtcTGCTGACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACttt                                                                                                            >  2:1466696/1‑138 (MQ=255)
                       cgtcTGCTGACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTc                                                                                                           >  1:61302/1‑138 (MQ=255)
                        gtcTGCTGACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCg                                                                                                          <  2:568346/138‑1 (MQ=255)
                         tcTGCTGACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGa                                                                                                         <  2:455477/138‑1 (MQ=255)
                           tgctgACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAGCAACGAAACTTTCGagc                                                                                                       <  2:1476600/138‑1 (MQ=255)
                           tgctgACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACtt                                                                                                             >  2:1815714/1‑132 (MQ=255)
                           tgctgACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACtt                                                                                                             <  1:1815714/132‑1 (MQ=255)
                           tgctgACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGagc                                                                                                       <  2:619087/138‑1 (MQ=255)
                           tgctgACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGagc                                                                                                       >  1:244610/1‑138 (MQ=255)
                           tgctgACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGagc                                                                                                       >  2:200065/1‑138 (MQ=255)
                           tgctgACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGagc                                                                                                       <  2:45439/138‑1 (MQ=255)
                               gACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCt                                                                                                   <  2:863347/138‑1 (MQ=255)
                                     tACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTctg                                                                                              >  2:469391/1‑137 (MQ=255)
                                     tACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTctg                                                                                              <  1:469391/137‑1 (MQ=255)
                                       cTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTctgctg                                                                                           >  2:456901/1‑138 (MQ=255)
                                          gcgTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATg                                                                                        <  1:394629/138‑1 (MQ=255)
                                          gcgTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATg                                                                                        <  2:818053/138‑1 (MQ=255)
                                           cgTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGc                                                                                       <  1:456901/138‑1 (MQ=255)
                                            gTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCa                                                                                      >  1:755220/1‑138 (MQ=255)
                                             tAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCaa                                                                                     >  1:1638415/1‑138 (MQ=255)
                                              aGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGCTATGAACCTAACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCaaa                                                                                    <  2:1638415/138‑1 (MQ=255)
                                              aGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGagcag                                                                                                     >  2:64975/1‑121 (MQ=255)
                                              aGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGagcag                                                                                                     <  1:64975/121‑1 (MQ=255)
                                              aGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCaaa                                                                                    <  2:1132645/138‑1 (MQ=255)
                                                aaaaaaCCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAg                                                                                  >  1:946076/1‑138 (MQ=255)
                                                 aaaaaCCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTTCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGc                                                                                 >  2:372591/1‑138 (MQ=255)
                                                 aaaaaCCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGc                                                                                 >  1:1487088/1‑138 (MQ=255)
                                                      ccTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTg                                                                            <  1:590278/138‑1 (MQ=255)
                                                        ttCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGt                                                                          <  1:47813/138‑1 (MQ=255)
                                                         tCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTg                                                                         <  2:636055/138‑1 (MQ=255)
                                                                   tGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTaa                                                               <  1:877578/138‑1 (MQ=255)
                                                                   tGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTaa                                                               >  2:963916/1‑138 (MQ=255)
                                                                    gATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTaaa                                                              <  1:1400515/138‑1 (MQ=255)
                                                                     aTTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAAt                                                             <  1:728569/138‑1 (MQ=255)
                                                                        ccGCTGAAGGCGCTGATGTTGTCTATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGgc                                                          <  2:490038/138‑1 (MQ=255)
                                                                        ccGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGgc                                                          <  2:202443/138‑1 (MQ=255)
                                                                        ccGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGgc                                                          <  2:1527809/138‑1 (MQ=255)
                                                                        ccGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGgc                                                          <  2:1756110/138‑1 (MQ=255)
                                                                        ccGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGgc                                                          <  2:102182/138‑1 (MQ=255)
                                                                        ccGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGgc                                                          <  1:970516/138‑1 (MQ=255)
                                                                        ccGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGgc                                                          <  1:1341499/138‑1 (MQ=255)
                                                                          gCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGgc                                                          <  2:1157948/136‑1 (MQ=255)
                                                                          gCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGgc                                                          >  1:1157948/1‑136 (MQ=255)
                                                                            tGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGgctgct                                                      <  2:773241/138‑1 (MQ=255)
                                                                                 gcgcTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTaa                                                               <  1:796399/124‑1 (MQ=255)
                                                                                 gcgcTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTaa                                                               >  2:796399/1‑124 (MQ=255)
                                                                                 gcgcTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATc                                                 >  1:768352/1‑138 (MQ=255)
                                                                                    cTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAgg                                              <  2:235537/138‑1 (MQ=255)
                                                                                    cTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAgg                                              <  1:1299426/138‑1 (MQ=255)
                                                                                    cTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAgg                                              <  2:1448420/138‑1 (MQ=255)
                                                                                       aTGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCag                                           <  2:1645874/138‑1 (MQ=255)
                                                                                       aTGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCag                                           >  1:1823322/1‑138 (MQ=255)
                                                                                        tgttgtCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCaga                                          <  1:221475/138‑1 (MQ=255)
                                                                                         gttgtCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCagag                                         <  2:660640/138‑1 (MQ=255)
                                                                                         gttgtCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCagag                                         <  1:288866/138‑1 (MQ=255)
                                                                                              cGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGc                                             <  2:526037/129‑1 (MQ=255)
                                                                                              cGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGc                                             >  1:526037/1‑129 (MQ=255)
                                                                                              cGATATGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTAt                                    <  2:61302/138‑1 (MQ=255)
                                                                                                 tatGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGt                                 <  1:200065/138‑1 (MQ=255)
                                                                                                 tatGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGt                                 <  2:768352/138‑1 (MQ=255)
                                                                                                   tGAACCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTaa                               >  1:1685144/1‑138 (MQ=255)
                                                                                                      aCCTGACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAActc                            >  2:272304/1‑138 (MQ=255)
                                                                                                          gACTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCtgtg                        <  2:961725/138‑1 (MQ=255)
                                                                                                           aCTTACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTgg                       >  2:442969/1‑138 (MQ=255)
                                                                                                             ttACCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGaa                     >  2:1718301/1‑138 (MQ=255)
                                                                                                               aCCTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATg                   <  2:1487088/138‑1 (MQ=255)
                                                                                                                 cTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGt                 >  1:1375358/1‑138 (MQ=255)
                                                                                                                 cTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGt                 >  1:256832/1‑138 (MQ=255)
                                                                                                                 cTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGt                 >  1:1492034/1‑138 (MQ=255)
                                                                                                                 cTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGt                 >  2:1473489/1‑138 (MQ=255)
                                                                                                                 cTGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGt                 >  2:565393/1‑138 (MQ=255)
                                                                                                                  tGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCg                                  <  2:591464/120‑1 (MQ=255)
                                                                                                                  tGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCg                                  >  1:591464/1‑120 (MQ=255)
                                                                                                                  tGTACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTc                >  2:1416743/1‑138 (MQ=255)
                                                                                                                    tACAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAg              >  1:1326609/1‑138 (MQ=255)
                                                                                                                     aCAACGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGc             <  2:1710087/138‑1 (MQ=255)
                                                                                                                       aaCGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGCCg           <  2:1375358/138‑1 (MQ=255)
                                                                                                                       aaCGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGCCg           >  2:1690157/1‑138 (MQ=255)
                                                                                                                       aaCGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGCCg           >  1:1706462/1‑138 (MQ=255)
                                                                                                                       aaCGACGGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGCCg           <  1:1454368/138‑1 (MQ=255)
                                                                                                                           acgGTGAGTCCTGGCACTTCATGCACAACGAAACTTTCGAGGAGGTGGCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGCCGAtct       >  2:83058/1‑138 (MQ=255)
                                                                                                                           acgGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGCCGAtct       >  2:453929/1‑138 (MQ=255)
                                                                                                                           acgGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGCCGAtct       >  2:1794397/1‑138 (MQ=255)
                                                                                                                           acgGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGCCGAtct       >  1:834625/1‑138 (MQ=255)
                                                                                                                           acgGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGCCGAtct       >  1:635941/1‑138 (MQ=255)
                                                                                                                           acgGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGCCGAtct       >  1:198447/1‑138 (MQ=255)
                                                                                                                           acgGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGCCGAtct       >  1:1265888/1‑138 (MQ=255)
                                                                                                                           acgGTGAGTCCTGGCACTTCATGAACAACGAAACTTCCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGc             >  2:1169289/1‑132 (MQ=255)
                                                                                                                           acgGTGAGTCCTGGCACTTCATGAACAACGAAACTTCCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGc             <  1:1169289/132‑1 (MQ=255)
                                                                                                                            cgGTGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGCCGAtctc      >  2:1793972/1‑138 (MQ=255)
                                                                                                                               tGAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGCCGATCTCCGt   <  2:834625/138‑1 (MQ=255)
                                                                                                                                gAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGCCGATCTCCGtt  >  1:207439/1‑138 (MQ=255)
                                                                                                                                gAGTCCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGCCGATCTCCGtt  >  1:182966/1‑138 (MQ=255)
                                                                                                                                    |                                                                                                                                     
CATTTGCTCGCGTTAAACTGCGTCGTCTGCTGACCGGTACTCGCGTAGAAAAAACCTTCAAATCTACTGATTCCGCTGAAGGCGCTGATGTTGTCGATATGAACCTGACTTACCTGTACAACGACGGTGAGTTCTGGCACTTCATGAACAACGAAACTTTCGAGCAGCTGTCTGCTGATGCAAAAGCAATTGGTGACAACGCTAAATGGCTGCTGGATCAGGCAGAGTGTATCGTAACTCTGTGGAATGGTCAGCCGATCTCCGTT  >  NC_000913/4375808‑4376073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A