Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 191 83.5 2830284 99.3% 2810472 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 76,698 A→G V201A (GTG→GCG)  sgrR ← DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091376,6980AG100.0% 208.5 / NA 57V201A (GTG→GCG) sgrRDNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/26);  total (31/26)

AACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCG  >  NC_000913/76562‑76834
                                                                                                                                        |                                                                                                                                        
aaCTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCAt                                                                                                                                       <  2:809826/140‑1 (MQ=255)
  cTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCg                                                                                                                                     <  1:580158/140‑1 (MQ=255)
     gCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTcc                                                                                                                                  <  2:589241/140‑1 (MQ=255)
          agcagTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCATGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTc                                                                                                                             <  2:1231937/140‑1 (MQ=255)
          agcagTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTc                                                                                                                             <  1:479945/140‑1 (MQ=255)
               tAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGtt                                                                                                                        <  2:715129/140‑1 (MQ=255)
                    gTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGa                                                                                                                   <  2:1276248/140‑1 (MQ=255)
                     tAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGAc                                                                                                                  >  1:214004/1‑140 (MQ=255)
                     tAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGAc                                                                                                                  <  1:335991/140‑1 (MQ=255)
                      aaCCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGAcc                                                                                                                 <  2:614746/140‑1 (MQ=255)
                       aCCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCa                                                                                                                >  1:49540/1‑140 (MQ=255)
                       aCCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCa                                                                                                                >  1:168939/1‑140 (MQ=255)
                            cGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGtt                                                                                                                        >  1:1051901/1‑127 (MQ=255)
                            cGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGtt                                                                                                                        <  2:1051901/127‑1 (MQ=255)
                              gTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGaa                                                                                                         >  2:1138846/1‑140 (MQ=255)
                                  ggTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATg                                                                                                     <  2:49540/140‑1 (MQ=255)
                                    ttGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGa                                                                                                   <  2:1048118/140‑1 (MQ=255)
                                         tGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCt                                                                                              >  1:1289979/1‑140 (MQ=255)
                                           agaTGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATTCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTgg                                                                                            >  1:916696/1‑140 (MQ=255)
                                            gaTGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATCGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGa                                                                                           <  2:897962/140‑1 (MQ=255)
                                                      cAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGa                                                                                 <  2:1289979/140‑1 (MQ=255)
                                                       aGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGaa                                                                                >  1:13573/1‑140 (MQ=255)
                                                         cGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCaa                                                                                      >  1:1294187/1‑132 (MQ=255)
                                                         cGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCaa                                                                                      <  2:1294187/132‑1 (MQ=255)
                                                          gTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACg                                                                             >  1:223306/1‑140 (MQ=255)
                                                           tCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGc                                                                            >  2:462022/1‑140 (MQ=255)
                                                           tCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGc                                                                            >  2:1282754/1‑140 (MQ=255)
                                                              aGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAg                                                                         >  1:518122/1‑140 (MQ=255)
                                                                     cGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGaa                                                                     >  1:943593/1‑137 (MQ=255)
                                                                     cGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGaa                                                                     <  2:943593/137‑1 (MQ=255)
                                                                     cGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCg                                                                  >  1:794689/1‑140 (MQ=255)
                                                                                  tCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTtgctg                                                     >  1:849338/1‑140 (MQ=255)
                                                                                  tCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTtgctg                                                     <  2:13573/140‑1 (MQ=255)
                                                                                   cAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGGCCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTtgctgc                                                    <  1:783244/140‑1 (MQ=255)
                                                                                     gCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCa                                                  >  2:478357/1‑140 (MQ=255)
                                                                                      cAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAg                                                 >  1:1049423/1‑140 (MQ=255)
                                                                                           tGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAgtggtg                                            >  1:1287193/1‑140 (MQ=255)
                                                                                           tGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAgtggtg                                            >  2:1353933/1‑140 (MQ=255)
                                                                                              gAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTtgctg                                                     <  2:969710/128‑1 (MQ=255)
                                                                                              gAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTtgctg                                                     >  1:969710/1‑128 (MQ=255)
                                                                                               aaTAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCg                                        <  2:223306/140‑1 (MQ=255)
                                                                                               aaTAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCg                                        <  1:928475/140‑1 (MQ=255)
                                                                                                aTAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGa                                       <  1:478357/140‑1 (MQ=255)
                                                                                                    agCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTc                                   >  1:1135810/1‑140 (MQ=255)
                                                                                                    agCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTc                                   >  1:278911/1‑140 (MQ=255)
                                                                                                     gCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCt                                  >  2:1333453/1‑140 (MQ=255)
                                                                                                      cGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTg                                 >  1:1250077/1‑140 (MQ=255)
                                                                                                         cAGCGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCtt                              >  1:509669/1‑140 (MQ=255)
                                                                                                            cGTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCa                           <  2:118173/140‑1 (MQ=255)
                                                                                                             gTATTGATTCGTTTTAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAg                          <  1:136274/140‑1 (MQ=255)
                                                                                                                        ttttAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCAtttt               >  1:965396/1‑140 (MQ=255)
                                                                                                                        ttttAAAGAGGCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCAtttt               >  1:33016/1‑140 (MQ=255)
                                                                                                                                  gCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTAt     <  2:794689/140‑1 (MQ=255)
                                                                                                                                  gCGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTAt     >  1:174836/1‑140 (MQ=255)
                                                                                                                                   cGATCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATg    <  2:1049423/140‑1 (MQ=255)
                                                                                                                                     aTCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATgcg  >  2:430210/1‑140 (MQ=255)
                                                                                                                                     aTCGCATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATgcg  >  2:647655/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
AACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCG  >  NC_000913/76562‑76834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A