Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 191 83.5 2830284 99.3% 2810472 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 82,968 A→G Y188H (TAT→CAT)  leuA ← 2‑isopropylmalate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091382,9680AG100.0% 342.5 / NA 92Y188H (TAT→CAT) leuA2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (48/44);  total (48/44)

CCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCG  >  NC_000913/82837‑83101
                                                                                                                                   |                                                                                                                                     
cccGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGc                                                                                                                               >  2:111692/1‑140 (MQ=255)
    ttCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCtgat                                                                                                                           >  1:347670/1‑140 (MQ=255)
    ttCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCtgat                                                                                                                           >  2:1369929/1‑140 (MQ=255)
    ttCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCtgat                                                                                                                           >  2:1089974/1‑140 (MQ=255)
    ttCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCtgat                                                                                                                           >  1:11526/1‑140 (MQ=255)
    ttCATTGCGCCTTCCACCTGGCGTGCACCAGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAATGCCAAATCGTGGTGGGTATGGACGGAGATAATGGCTTTGTCGATGGTAGGCACGCGTTCATGCAAGCCGCtgtt                                                                                                                           >  2:171154/1‑138 (MQ=255)
         tGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTc                                                                                                                      >  1:779776/1‑140 (MQ=255)
         tGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGGATGTACGGAGGTAATGGCTTTGTCGATGGTAGGCACGCGGTCATGCAGGCCGCTGATGAttt                                                                                                                      >  1:981796/1‑139 (MQ=255)
           cgcCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCg                                                                                                                    >  2:432830/1‑140 (MQ=255)
                    ccTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCa                                                                                                           <  1:1311476/140‑1 (MQ=255)
                        gCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACg                                                                                                       <  2:571374/140‑1 (MQ=255)
                          gTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGc                                                                                                     <  1:1369929/140‑1 (MQ=255)
                           tGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCa                                                                                                    <  1:111692/140‑1 (MQ=255)
                           tGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCa                                                                                                    >  2:1000360/1‑140 (MQ=255)
                           tGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCa                                                                                                    <  2:1174847/140‑1 (MQ=255)
                           tGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCa                                                                                                    <  1:153857/140‑1 (MQ=255)
                           tGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCa                                                                                                    <  2:11526/140‑1 (MQ=255)
                           tGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCa                                                                                                    <  1:137525/140‑1 (MQ=255)
                           tGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCa                                                                                                    >  1:1372210/1‑140 (MQ=255)
                           tGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCa                                                                                                    <  2:1101409/140‑1 (MQ=255)
                           tGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCa                                                                                                    <  2:611953/140‑1 (MQ=255)
                           tGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCa                                                                                                    <  2:521399/140‑1 (MQ=255)
                           tGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCa                                                                                                    <  2:54569/140‑1 (MQ=255)
                           tGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCACTGATGATTCCGGCGAACTCAAACGGCa                                                                                                    <  2:309400/140‑1 (MQ=255)
                             cACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGc                                                                                                     <  1:1081319/137‑1 (MQ=255)
                             cACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGc                                                                                                     >  2:1081319/1‑137 (MQ=255)
                               ccGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGgt                                                                                                <  1:734552/140‑1 (MQ=255)
                               ccGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGgt                                                                                                <  1:1139145/140‑1 (MQ=255)
                                cGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGgtg                                                                                               <  1:716217/140‑1 (MQ=255)
                                cGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGgtg                                                                                               <  2:1194499/140‑1 (MQ=255)
                                  gCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTa                                                                                             >  2:159114/1‑140 (MQ=255)
                                          cgccgcCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACgg                                                                                     >  2:178456/1‑140 (MQ=255)
                                           gccgccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGgt                                                                                    <  2:779776/140‑1 (MQ=255)
                                           gccgccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGgt                                                                                    <  2:1084332/140‑1 (MQ=255)
                                            ccgccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCAc                                                                                       >  2:126193/1‑136 (MQ=255)
                                            ccgccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCAc                                                                                       <  1:126193/136‑1 (MQ=255)
                                            ccgccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGgtg                                                                                   >  2:399089/1‑140 (MQ=255)
                                            ccgccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGgtg                                                                                   >  2:715062/1‑140 (MQ=255)
                                            ccgccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACCCAAACGGCATGGTGTAGccc                                                                                         >  1:704735/1‑134 (MQ=255)
                                            ccgccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACCCAAACGGCATGGTGTAGccc                                                                                         <  2:704735/134‑1 (MQ=255)
                                               ccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTcc                                                                                >  2:1057555/1‑140 (MQ=255)
                                               ccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTcc                                                                                >  1:1292360/1‑140 (MQ=255)
                                                 aGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCgg                                                                              >  1:1387940/1‑140 (MQ=255)
                                                 aGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCgg                                                                              <  1:1174443/140‑1 (MQ=255)
                                                  gTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGa                                                                             <  1:1057555/140‑1 (MQ=255)
                                                    gAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAAt                                                                           <  1:157943/140‑1 (MQ=255)
                                                       tttCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGtt                                                                        >  2:1362699/1‑140 (MQ=255)
                                                                 ccAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGAtggtggtg                                                              >  1:631018/1‑140 (MQ=255)
                                                                    ggCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCa                                                           >  1:894235/1‑140 (MQ=255)
                                                                    ggCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCa                                                           <  1:959461/140‑1 (MQ=255)
                                                                    ggCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCa                                                           <  1:373786/140‑1 (MQ=255)
                                                                                cgtGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATc                                               >  2:716880/1‑140 (MQ=255)
                                                                                 gtGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATcg                                              <  1:1088191/140‑1 (MQ=255)
                                                                                 gtGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATcg                                              <  1:1362699/140‑1 (MQ=255)
                                                                                          tACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGa                                     >  1:576972/1‑140 (MQ=255)
                                                                                              gagaTAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCAc                                 <  2:973463/140‑1 (MQ=255)
                                                                                              gagaTAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCAc                                 <  2:631018/140‑1 (MQ=255)
                                                                                               agaTAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCATGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACt                                >  1:92008/1‑140 (MQ=255)
                                                                                               agaTAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACt                                >  1:1062944/1‑140 (MQ=255)
                                                                                               agaTAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACt                                >  1:830092/1‑140 (MQ=255)
                                                                                               agaTAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACt                                >  2:570139/1‑140 (MQ=255)
                                                                                               agaTAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACt                                >  2:1134781/1‑140 (MQ=255)
                                                                                               agaTAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACt                                >  2:353517/1‑140 (MQ=255)
                                                                                                gaTAATGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTc                               >  1:745246/1‑140 (MQ=255)
                                                                                                   aaTGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTcgcg                            >  2:211460/1‑140 (MQ=255)
                                                                                                    aTGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCgcgc                           >  2:56747/1‑140 (MQ=255)
                                                                                                    aTGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCgcgc                           >  2:145443/1‑140 (MQ=255)
                                                                                                    aTGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCgcgc                           >  1:1252291/1‑140 (MQ=255)
                                                                                                    aTGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCgcgc                           >  1:1402002/1‑140 (MQ=255)
                                                                                                    aTGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCgcgc                           >  2:877048/1‑140 (MQ=255)
                                                                                                    aTGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCgcgc                           >  2:1093264/1‑140 (MQ=255)
                                                                                                    aTGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGACATTAATCGCCGCTTCGACCACTCgcgc                           >  1:107024/1‑140 (MQ=255)
                                                                                                     tGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTcgcg                            >  2:1337604/1‑138 (MQ=255)
                                                                                                     tGGCTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTcgcg                            <  1:1337604/138‑1 (MQ=255)
                                                                                                        cTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCGCCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGa                       <  2:1007939/140‑1 (MQ=255)
                                                                                                        cTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGa                       <  1:1089974/140‑1 (MQ=255)
                                                                                                        cTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGa                       <  1:1093264/140‑1 (MQ=255)
                                                                                                        cTTTGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGa                       >  1:141470/1‑140 (MQ=255)
                                                                                                         tttGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGAt                      >  1:546074/1‑140 (MQ=255)
                                                                                                          ttGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATc                     <  2:327136/140‑1 (MQ=255)
                                                                                                           tGTCGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCg                    >  1:19460/1‑140 (MQ=255)
                                                                                                              cGATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCa                 <  1:1000360/140‑1 (MQ=255)
                                                                                                               gATGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCaa                <  1:676969/140‑1 (MQ=255)
                                                                                                                aTGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAAt               <  1:211460/140‑1 (MQ=255)
                                                                                                                aTGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAAt               <  1:1348209/140‑1 (MQ=255)
                                                                                                                aTGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAAt               <  2:347670/140‑1 (MQ=255)
                                                                                                                aTGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAAt               <  1:432830/140‑1 (MQ=255)
                                                                                                                aTGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAAt               <  1:193692/140‑1 (MQ=255)
                                                                                                                aTGTTAGGCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACAGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAAt               <  2:981796/140‑1 (MQ=255)
                                                                                                                       gCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTa        <  2:489448/140‑1 (MQ=255)
                                                                                                                       gCACGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTa        >  1:107543/1‑140 (MQ=255)
                                                                                                                         aCGCGTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACg      >  1:744382/1‑140 (MQ=255)
                                                                                                                          cgcgTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGc     >  2:923893/1‑140 (MQ=255)
                                                                                                                             gTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCg  >  1:58814/1‑140 (MQ=255)
                                                                                                                             gTTCATGCAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCg  >  1:508261/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                     
CCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCG  >  NC_000913/82837‑83101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A