Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	136,500	(A)_7→8	intergenic (‑36/+70)	speE ← / ← yacC	spermidine synthase/putative lipoprotein YacC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	136,493	1	.	A	96.7%	312.1 / 2.1	90	intergenic (‑29/+77)	speE/yacC	spermidine synthase/putative lipoprotein YacC
Reads supporting (aligned to +/- strand): ref base . (1/2); new base A (45/42); total (46/44)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

AATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGT‑AAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTT  >  NC_000913/136354‑136626

aaTGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGt                                                                                                                                         >  2:363013/1‑140 (MQ=255)

           ccTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATTCCTCCTTTGTTAACACCCGT‑AAAAA‑AAaggg                                                                                                                            <  2:461514/140‑2 (MQ=255)

           ccTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGT‑aaaaa‑aaaggg                                                                                                                            <  1:454436/140‑5 (MQ=255)

           ccTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGT‑aaaaa‑aaaggg                                                                                                                            <  2:476932/140‑5 (MQ=255)

           ccTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGT‑aaaaa‑aaaggg                                                                                                                            <  1:908939/140‑5 (MQ=255)

             tGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGgcg                                                                                                                           <  1:1007128/140‑1 (MQ=255)

             tGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGgcg                                                                                                                           <  1:610065/140‑1 (MQ=255)

             tGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGgcg                                                                                                                           <  2:410026/140‑1 (MQ=255)

             tGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGgcg                                                                                                                           <  1:689842/140‑1 (MQ=255)

              ggTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGgcgc                                                                                                                          <  2:1320858/140‑1 (MQ=255)

              ggTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGgcgc                                                                                                                          <  1:478541/140‑1 (MQ=255)

              ggTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGgcgc                                                                                                                          >  2:1261939/1‑140 (MQ=255)

              ggTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGgcg                                                                                                                           <  1:700516/139‑1 (MQ=255)

              ggTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGgcg                                                                                                                           >  2:700516/1‑139 (MQ=255)

              ggTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGT‑aaaaa‑a                                                                                                                                 >  2:1353773/1‑132 (MQ=255)

              ggTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGT‑aaaaa‑a                                                                                                                                 <  1:1353773/132‑1 (MQ=255)

               gTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCa                                                                                                                         <  1:125078/140‑1 (MQ=255)

               gTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCa                                                                                                                         >  2:382443/1‑140 (MQ=255)

               gTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCa                                                                                                                         >  1:429270/1‑140 (MQ=255)

               gTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTACTTTGTTAACACCCGTAAAAAA‑AAGGGCGCa                                                                                                                         <  2:715189/140‑1 (MQ=255)

                  aTCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACa                                                                                                                      <  1:70953/140‑1 (MQ=255)

                   tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGgcg                                                                                                                           <  1:799798/134‑1 (MQ=255)

                   tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGgcg                                                                                                                           >  2:799798/1‑134 (MQ=255)

                   tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACat                                                                                                                     >  1:900208/1‑140 (MQ=255)

                    cGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACata                                                                                                                    >  2:81618/1‑140 (MQ=255)

                    cGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACata                                                                                                                    >  2:1378965/1‑140 (MQ=255)

                    cGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACata                                                                                                                    <  1:1378965/140‑1 (MQ=255)

                     ggTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACataa                                                                                                                   >  1:378103/1‑140 (MQ=255)

                        cTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAg                                                                                                                <  1:363013/140‑1 (MQ=255)

                         ttttCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGc                                                                                                               >  1:1022492/1‑140 (MQ=255)

                           ttCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTa                                                                                                             <  2:900208/140‑1 (MQ=255)

                                   aCAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGAc                                                                                                     <  2:1360532/140‑1 (MQ=255)

                                   aCAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGAc                                                                                                     <  1:942463/140‑1 (MQ=255)

                                     aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACcg                                                                                                   >  1:326542/1‑140 (MQ=255)

                                     aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACcg                                                                                                   >  2:496157/1‑140 (MQ=255)

                                     aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACcg                                                                                                   >  1:1174567/1‑140 (MQ=255)

                                     aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACcg                                                                                                   >  2:1288231/1‑140 (MQ=255)

                                     aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACcg                                                                                                   >  1:1409201/1‑140 (MQ=255)

                                      gAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACcg                                                                                                   <  1:917616/140‑1 (MQ=255)

                                       aaCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACcgcg                                                                                                 >  1:1098710/1‑140 (MQ=255)

                                       aaCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACcgcg                                                                                                 >  1:462072/1‑140 (MQ=255)

                                       aaCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACcgcg                                                                                                 >  1:79499/1‑140 (MQ=255)

                                       aaCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACcgcg                                                                                                 >  1:769379/1‑140 (MQ=255)

                                          gTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGAt                                                                                              >  2:6349/1‑140 (MQ=255)

                                            tATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGc                                                                                            >  1:123089/1‑140 (MQ=255)

                                            tATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGc                                                                                            >  1:1188389/1‑140 (MQ=255)

                                            tATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCAAGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGc                                                                                            <  2:769379/140‑1 (MQ=255)

                                             aTCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                                                                           <  1:1288231/140‑1 (MQ=255)

                                             aTCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                                                                           <  2:514193/140‑1 (MQ=255)

                                             aTCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                                                                           >  2:689740/1‑140 (MQ=255)

                                             aTCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                                                                           <  1:1144264/140‑1 (MQ=255)

                                             aTCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                                                                           <  1:689740/140‑1 (MQ=255)

                                              tCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCAc                                                                                          >  1:1000806/1‑140 (MQ=255)

                                              tCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCAc                                                                                          <  2:326542/140‑1 (MQ=255)

                                               cTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACg                                                                                         <  1:457057/140‑1 (MQ=255)

                                                 aCCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGt                                                                                       >  1:1183000/1‑140 (MQ=255)

                                                 aCCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGt                                                                                       >  2:307797/1‑140 (MQ=255)

                                                 aCCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGt                                                                                       >  2:47851/1‑140 (MQ=255)

                                                  ccGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTc                                                                                      <  2:1209957/140‑1 (MQ=255)

                                                   cGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCa                                                                                     >  1:1109068/1‑140 (MQ=255)

                                                     cAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAat                                                                                   >  1:836100/1‑140 (MQ=255)

                                                        aGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATAtt                                                                                >  1:43789/1‑140 (MQ=255)

                                                         gTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATAttt                                                                               <  1:1359299/140‑1 (MQ=255)

                                                             tGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                                                                           >  2:664449/1‑124 (MQ=255)

                                                             tGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                                                                           <  1:664449/124‑1 (MQ=255)

                                                             tGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTAc                                                                           <  2:1188389/140‑1 (MQ=255)

                                                              gCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACa                                                                          <  1:30414/140‑1 (MQ=255)

                                                               cccAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACaa                                                                         <  2:1183000/140‑1 (MQ=255)

                                                                ccAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACaaa                                                                        <  1:307797/140‑1 (MQ=255)

                                                                    aCTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGT‑AAAAAGAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAgg                                                                    >  1:1261584/1‑140 (MQ=255)

                                                                       ggTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTAt                                                                 <  2:462072/140‑1 (MQ=255)

                                                                       ggTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGT‑AAAAAGAATGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTAt                                                                 <  2:1261584/140‑1 (MQ=255)

                                                                         tCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCa                                                               >  1:1212230/1‑140 (MQ=255)

                                                                         tCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCa                                                               >  1:1402283/1‑140 (MQ=255)

                                                                                  gTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGattatt                                                      >  1:434798/1‑140 (MQ=255)

                                                                                        tGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGGGCAACATTAATGGTAACATTGACCGCGGGTTCACGGTCAATATTTTTACAAAAAAGGATCAGGGATTATTTGACgg                                                >  2:6188/1‑139 (MQ=255)

                                                                                        tGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGt                                                >  1:103144/1‑140 (MQ=255)

                                                                                          ccACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAg                                              <  2:1174567/140‑1 (MQ=255)

                                                                                          ccACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAg                                              >  2:374431/1‑140 (MQ=255)

                                                                                              tG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCaa                                          >  1:805561/1‑140 (MQ=255)

                                                                                               g‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAg                                         <  1:836236/140‑1 (MQ=255)

                                                                                               g‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAg                                         <  2:79499/140‑1 (MQ=255)

                                                                                               g‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAg                                         <  2:1109068/140‑1 (MQ=255)

                                                                                                 ttttttttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGc                                        <  1:125671/140‑1 (MQ=255)

                                                                                                 ttttttttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGc                                        <  1:374431/140‑1 (MQ=255)

                                                                                                  tttttttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCa                                       <  1:496157/140‑1 (MQ=255)

                                                                                                                gggTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATcg                         <  1:81618/140‑1 (MQ=255)

                                                                                                                gggTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATcg                         <  2:123089/140‑1 (MQ=255)

                                                                                                                gggTTGATACCTCCTTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATcg                         <  1:382443/140‑1 (MQ=255)

                                                                                                                           tcctTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGc              >  2:1312543/1‑140 (MQ=255)

                                                                                                                           tcctTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTAAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGc              >  2:1284039/1‑140 (MQ=255)

                                                                                                                            cctTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCt             >  2:50132/1‑140 (MQ=255)

                                                                                                                            cctTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCt             >  2:1093122/1‑140 (MQ=255)

                                                                                                                            cctTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCt             >  1:1250598/1‑140 (MQ=255)

                                                                                                                             ctTTGTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCtt            >  1:528738/1‑140 (MQ=255)

                                                                                                                                 gTTAACACCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCa        >  1:482371/1‑140 (MQ=255)

                                                                                                                                       acCCGTAAAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCAtttttt  >  1:672180/1‑140 (MQ=255)

AATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGT‑AAAAA‑AAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTT  >  NC_000913/136354‑136626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A