Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	189,931	A→G	T20A (ACC→GCC)	rpsB →	30S ribosomal subunit protein S2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	189,931	0	A	G	99.0%	360.2 / ‑3.7	101	coding (58/726 nt)	rpsB	30S ribosomal subunit protein S2
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (52/48); minor base . (0/1); total (52/49)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 101 total aligned reads displayed.

ATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGCTGAACTGAACAAGATTGCTTCTCGCA  >  NC_000913/189796‑190063

atTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCg                                                                                                                                  >  2:16132/1‑140 (MQ=255)

atTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCg                                                                                                                                  >  2:1154739/1‑140 (MQ=255)

    cGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTAc                                                                                                                              >  1:482039/1‑140 (MQ=255)

    cGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTAc                                                                                                                              >  1:400548/1‑140 (MQ=255)

       ggTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTgg                                                                                                                           <  2:482039/140‑1 (MQ=255)

         tGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGaa                                                                                                                         >  2:1275296/1‑140 (MQ=255)

         tGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGaa                                                                                                                         >  1:1367683/1‑140 (MQ=255)

          gCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAAc                                                                                                                        >  2:220278/1‑140 (MQ=255)

           cccTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAAcc                                                                                                                       >  2:67855/1‑140 (MQ=255)

           cccTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAAcc                                                                                                                       <  1:477364/140‑1 (MQ=255)

            ccTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAAccc                                                                                                                      >  2:217115/1‑140 (MQ=255)

                    gTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGa                                                                                                              <  1:957539/140‑1 (MQ=255)

                     tCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGa                                                                                                              <  2:1195026/139‑1 (MQ=255)

                     tCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGa                                                                                                              >  1:1195026/1‑139 (MQ=255)

                      cGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGGGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAg                                                                                                            >  1:1381720/1‑140 (MQ=255)

                         tAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCg                                                                                                         <  1:794040/140‑1 (MQ=255)

                           atatGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGtt                                                                                                       <  2:140341/140‑1 (MQ=255)

                           atatGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGtt                                                                                                       <  2:1381720/140‑1 (MQ=255)

                               gggATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATc                                                                                                   >  1:1267841/1‑140 (MQ=255)

                               gggATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATc                                                                                                   >  2:659387/1‑140 (MQ=255)

                                 gATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAg                                                                                                            <  2:108392/129‑1 (MQ=255)

                                 gATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAg                                                                                                            >  1:108392/1‑129 (MQ=255)

                                   tACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCg                                                                                               >  2:1126898/1‑140 (MQ=255)

                                   tACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCg                                                                                               >  1:761362/1‑140 (MQ=255)

                                     cGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGt                                                                                             >  2:482362/1‑140 (MQ=255)

                                     cGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGt                                                                                             <  2:564696/140‑1 (MQ=255)

                                     cGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGt                                                                                             >  2:582469/1‑140 (MQ=255)

                                     cGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGt                                                                                             <  2:604594/140‑1 (MQ=255)

                                     cGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGt                                                                                             <  1:894661/140‑1 (MQ=255)

                                     cGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGt                                                                                             <  1:1154739/140‑1 (MQ=255)

                                       tGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTgc                                                                                           >  1:196046/1‑140 (MQ=255)

                                        ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTgcg                                                                                          >  1:599828/1‑140 (MQ=255)

                                         gAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTgcgc                                                                                         >  1:1084322/1‑140 (MQ=255)

                                          aGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGcgcg                                                                                        >  2:372095/1‑140 (MQ=255)

                                          aGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGcgcg                                                                                        >  2:1080347/1‑140 (MQ=255)

                                          aGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGcgcg                                                                                        <  2:761362/140‑1 (MQ=255)

                                           ggCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGt                                                                                       >  2:779221/1‑140 (MQ=255)

                                           ggCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGt                                                                                       >  2:543604/1‑140 (MQ=255)

                                           ggCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGt                                                                                       >  1:77688/1‑140 (MQ=255)

                                             cATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTaa                                                                                     <  1:582469/140‑1 (MQ=255)

                                               tAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCGGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACa                                                                                   >  2:240058/1‑140 (MQ=255)

                                               tAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACa                                                                                   >  1:1110698/1‑140 (MQ=255)

                                                 aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACaaa                                                                                 >  1:1365196/1‑140 (MQ=255)

                                                 aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAG‑GCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAg                                                                                <  2:388030/140‑1 (MQ=255)

                                                  ccccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAg                                                                                <  2:77688/140‑1 (MQ=255)

                                                  ccccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAg                                                                                >  2:632064/1‑140 (MQ=255)

                                                    ccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt                                                                              >  1:957626/1‑140 (MQ=255)

                                                     cAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTc                                                                             <  1:309426/140‑1 (MQ=255)

                                                      aaCTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTca                                                                            <  2:400548/140‑1 (MQ=255)

                                                      aaCTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTca                                                                            <  1:217115/140‑1 (MQ=255)

                                                      aaCTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTca                                                                            <  1:67855/140‑1 (MQ=255)

                                                      aaCTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTca                                                                            <  1:481389/140‑1 (MQ=255)

                                                      aaCTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTca                                                                            <  2:1131309/140‑1 (MQ=255)

                                                       aCTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTcac                                                                           <  1:468035/140‑1 (MQ=255)

                                                       aCTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTcac                                                                           <  1:1188789/140‑1 (MQ=255)

                                                           ttATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatca                                                                       <  2:1367683/140‑1 (MQ=255)

                                                            tatataGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatcat                                                                      >  2:436499/1‑140 (MQ=255)

                                                            tatataGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatcat                                                                      <  2:1180342/140‑1 (MQ=255)

                                                            tatataGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatcat                                                                      <  2:1110698/140‑1 (MQ=255)

                                                             atataGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACatcatc                                                                     >  1:420567/1‑140 (MQ=255)

                                                                taGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAAc                                                                  <  1:1275296/140‑1 (MQ=255)

                                                                taGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAAc                                                                  <  2:1365196/140‑1 (MQ=255)

                                                                  gagGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCt                                                                >  1:811397/1‑140 (MQ=255)

                                                                  gagGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCt                                                                >  1:842336/1‑140 (MQ=255)

                                                                   agGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCtt                                                               >  1:334245/1‑140 (MQ=255)

                                                                   agGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCtt                                                               >  1:608748/1‑140 (MQ=255)

                                                                   agGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCtt                                                               >  2:1368782/1‑140 (MQ=255)

                                                                   agGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCtt                                                               >  1:34760/1‑140 (MQ=255)

                                                                       tttAATCATGGCAACTGTTTCCATGTGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTgaga                                                           <  2:137037/140‑1 (MQ=255)

                                                                        ttAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGaa                                                          <  1:627745/140‑1 (MQ=255)

                                                                        ttAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGaa                                                          <  2:599828/140‑1 (MQ=255)

                                                                        ttAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGaa                                                          <  1:659387/140‑1 (MQ=255)

                                                                          aaTCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGaaaa                                                        <  1:1126898/140‑1 (MQ=255)

                                                                             cATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTg                                                     <  1:220278/140‑1 (MQ=255)

                                                                               tGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGaaaa                                                        >  2:939802/1‑135 (MQ=255)

                                                                               tGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGaaaa                                                        <  1:939802/135‑1 (MQ=255)

                                                                               tGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTa                                                   >  2:549114/1‑140 (MQ=255)

                                                                                ggCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTAc                                                  <  2:1128876/140‑1 (MQ=255)

                                                                                ggCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTAc                                                  <  2:196046/140‑1 (MQ=255)

                                                                                          tCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCaa                                        >  1:993664/1‑140 (MQ=255)

                                                                                                cgcgACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGc                                  <  2:420567/140‑1 (MQ=255)

                                                                                                      aTGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGc                            <  2:842336/140‑1 (MQ=255)

                                                                                                      aTGCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGc                            <  1:16132/140‑1 (MQ=255)

                                                                                                        gCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGctg                          >  1:524246/1‑140 (MQ=255)

                                                                                                        gCTCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGctg                          <  2:213628/140‑1 (MQ=255)

                                                                                                          tCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGctgaa                        <  2:34760/140‑1 (MQ=255)

                                                                                                          tCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGctgaa                        <  2:334245/140‑1 (MQ=255)

                                                                                                          tCAAGGCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGctgaa                        <  2:1084322/140‑1 (MQ=255)

                                                                                                               gCTGGTGTTCACTTCGGTCACCGGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGctgaactgaa                   <  1:240058/140‑1 (MQ=255)

                                                                                                               gCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGctgaactgaa                   <  1:984056/140‑1 (MQ=255)

                                                                                                               gCTGGTGTTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGctgaactgaa                   >  1:418277/1‑140 (MQ=255)

                                                                                                                   gtgtTCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGCTGAACTGAACAAg               >  2:441758/1‑140 (MQ=255)

                                                                                                                      ttCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTgg                             <  2:341137/123‑1 (MQ=255)

                                                                                                                      ttCACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTgg                             >  1:341137/1‑123 (MQ=255)

                                                                                                                        cACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGCTGAACTGAACAAGATTGc          >  1:1319832/1‑140 (MQ=255)

                                                                                                                        cACTTCGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGCTGAACTGAACAAGATTGc          >  1:625880/1‑140 (MQ=255)

                                                                                                                             cGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGCTGAACTGAACAAGATTGCTtctc     >  1:1202488/1‑140 (MQ=255)

                                                                                                                             cGGTCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGCTGAACTGAACAAGATTGCTtctc     >  2:631442/1‑140 (MQ=255)

                                                                                                                                tCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGCTGAACTGAACAAGATTGCTTCTCGCa  <  2:993664/140‑1 (MQ=255)

                                                                                                                                tCACCAGGCCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGCTGAACTGAACAAGATTGCTTCTCGCa  >  2:142612/1‑140 (MQ=255)

ATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGCTGAACTGAACAAGATTGCTTCTCGCA  >  NC_000913/189796‑190063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A