Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,496,601	A→G	E438E (GAA→GAG)	ydcJ →	DUF1338 domain‑containing protein YdcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,496,601	0	A	G	100.0%	180.9 / NA	50	E438E (GAA→GAG)	ydcJ	DUF1338 domain‑containing protein YdcJ
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/23); total (27/23)

GGGATCTTCCAGTCAAATCTGGGTAATGAAACGCAGACACGCAGTCACGGTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAACGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTC  >  NC_000913/1496470‑1496736

gggATCTTCCAGTCACATCTGGGTAATGAAACGCAGACACGCAGTCACGGTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTaa                                                                                                                                 >  1:698031/1‑140 (MQ=255)

   aTCTTCCAGTCAAATCTGGGTAATGAAACGCAGACATGCAGTCACGGTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACg                                                                                                                              <  1:1028464/140‑1 (MQ=255)

           gTCAAATCTGGGTAATGAAACGCAGACACGCAGTCACGGTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGc                                                                                                                          >  1:17196/1‑136 (MQ=255)

           gTCAAATCTGGGTAATGAAACGCAGACACGCAGTCACGGTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGc                                                                                                                          <  2:17196/136‑1 (MQ=255)

           gTCAAATCTGGGTAATGAAACGCAGACACGCAGTCACGGTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCtgtg                                                                                                                      >  2:230231/1‑140 (MQ=255)

           gTCAAATCTGGGTAATGAAACGCAGACACGCAGTCACGGTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCtgtg                                                                                                                      >  1:495407/1‑140 (MQ=255)

            tCAAATCTGGGTAATGAAACGCAGACACGCAGTCACGGTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTgg                                                                                                                     >  1:1070924/1‑140 (MQ=255)

             cAAATCTGGGTAATGAAACGCAGACACGCAGTCACGGTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGt                                                                                                                    <  1:1210111/140‑1 (MQ=255)

              aaaTCTGGGTAATGAAACGCAGACACGCAGTCACGGTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGtt                                                                                                                   <  1:230231/140‑1 (MQ=255)

                   tGGGTAATGAAACGCAGACACGCAGTCACGGTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCtt                                                                                                              <  2:587201/140‑1 (MQ=255)

                          tGAAACGCAGACACGCAGTCACGGTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATc                                                                                                       >  2:315908/1‑140 (MQ=255)

                           gAAACGCAGACACGCAGTCACGGTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCt                                                                                                      >  1:675932/1‑140 (MQ=255)

                               cGCAGACACGCAGTCACGGTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGAcc                                                                                                  >  2:1264336/1‑140 (MQ=255)

                                     cacGCAGTCACGGTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGc                                                                                            <  1:1334913/140‑1 (MQ=255)

                                                ggTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCaaaa                                                                                  >  1:120350/1‑139 (MQ=255)

                                                ggTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCaaaa                                                                                  <  2:120350/139‑1 (MQ=255)

                                                  tAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAAtt                                                                               >  2:620120/1‑140 (MQ=255)

                                                     tGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGc                                                                            <  1:1186430/140‑1 (MQ=255)

                                                         aGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGc                                                                                      <  2:1178722/126‑1 (MQ=255)

                                                         aGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGc                                                                                      >  1:1178722/1‑126 (MQ=255)

                                                           tCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGAtt                                                                      >  2:153818/1‑140 (MQ=255)

                                                            cgcgAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCt                                                                          >  2:1235251/1‑135 (MQ=255)

                                                            cgcgAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCt                                                                          <  1:1068019/135‑1 (MQ=255)

                                                            cgcgAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCt                                                                          <  1:1235251/135‑1 (MQ=255)

                                                            cgcgAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCt                                                                          >  2:1068019/1‑135 (MQ=255)

                                                                 aGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACaa                                                                <  2:1070924/140‑1 (MQ=255)

                                                                     tttGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGa                                                            >  2:248808/1‑140 (MQ=255)

                                                                       tGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAAt                                                          <  2:946841/140‑1 (MQ=255)

                                                                           cAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAgg                                                      <  2:698031/140‑1 (MQ=255)

                                                                             ggCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCt                                                    <  1:620120/140‑1 (MQ=255)

                                                                             ggCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCt                                                    >  2:540487/1‑140 (MQ=255)

                                                                                        gTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGa                                         >  1:83709/1‑140 (MQ=255)

                                                                                        gTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGa                                         >  1:811018/1‑140 (MQ=255)

                                                                                        gTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGa                                         >  1:496793/1‑140 (MQ=255)

                                                                                         tCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGAc                                        >  1:852092/1‑140 (MQ=255)

                                                                                         tCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGAc                                        >  2:1194017/1‑140 (MQ=255)

                                                                                         tCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGAc                                        >  1:1394643/1‑140 (MQ=255)

                                                                                         tCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGAc                                        >  1:1072930/1‑140 (MQ=255)

                                                                                              ttttGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCGGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATaaa                                   >  2:554325/1‑140 (MQ=255)

                                                                                                     tGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATg                            <  1:1264336/140‑1 (MQ=255)

                                                                                                     tGAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATg                            <  2:1394643/140‑1 (MQ=255)

                                                                                                      gAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGc                           <  1:1194017/140‑1 (MQ=255)

                                                                                                      gAGTTCCAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGc                           <  1:540487/140‑1 (MQ=255)

                                                                                                           ccAGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGAtt                      <  2:1072930/140‑1 (MQ=255)

                                                                                                             aGCTTTACCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTa                    <  1:1012251/140‑1 (MQ=255)

                                                                                                                   aCCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGcc                          <  1:1158376/128‑1 (MQ=255)

                                                                                                                   aCCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGcc                          >  2:1158376/1‑128 (MQ=255)

                                                                                                                   aCCAGGAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCata              >  2:4636/1‑140 (MQ=255)

                                                                                                                       ggAAGCGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATtct          <  1:884404/140‑1 (MQ=255)

                                                                                                                            cGGAAGAGCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAg     >  2:1243463/1‑140 (MQ=255)

                                                                                                                              gaagagCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGtt   <  1:153818/140‑1 (MQ=255)

                                                                                                                              gaagagCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGtt   <  2:83709/140‑1 (MQ=255)

                                                                                                                               aagagCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTc  >  1:223553/1‑140 (MQ=255)

                                                                                                                               aagagCGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTc  <  2:852092/140‑1 (MQ=255)

GGGATCTTCCAGTCAAATCTGGGTAATGAAACGCAGACACGCAGTCACGGTAATGCCAGTCGCGAAGCATTTGAGCAGGCGTTGGGTTGTCCGGTTTTGGATGAGTTCCAGCTTTACCAGGAAGCGGAAGAACGCAGTAAACGTCGCTGTGGTTTGCTTTAAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTC  >  NC_000913/1496470‑1496736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A