Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,680,325	G→A	A257T (GCA→ACA)	ydgI →	putative arginine:ornithine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,680,325	0	G	A	100.0%	160.2 / NA	50	A257T (GCA→ACA)	ydgI	putative arginine:ornithine antiporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/25); total (25/25)

ATTGGTGTGGAAGGTGCCGTGGTTGTTTCTGCGCGTGCGCGTAATAAACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACC  >  NC_000913/1680190‑1680459

aTTGGTGTGGAAGGTGCCGTGGTTGTTTCTGCGCGTGCGCGTAATAAACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACg                                                                                                                                    <  2:1166660/140‑1 (MQ=255)

            ggTGCCGTGGTTGTTTCTGCGCGTGCGCGTAATAAACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGaa                                                                                                                             >  2:751828/1‑135 (MQ=255)

            ggTGCCGTGGTTGTTTCTGCGCGTGCGCGTAATAAACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGaa                                                                                                                             <  1:751828/135‑1 (MQ=255)

               gCCGTGGTTGTTTCTGCGCGTGCGCGTAATAAACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGa                                                                                                                     >  1:1088338/1‑140 (MQ=255)

               gCCGTGGTTGTTTCTGCGCGTGCGCGTAATAAACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGa                                                                                                                     >  1:1359088/1‑140 (MQ=255)

               gCCGTGGTTGTTTCTGCGCGTGCGCGTAATAAACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGGGTGGTGACACGTCCTGAACTGGCTGa                                                                                                                     >  2:800055/1‑140 (MQ=255)

                 cGTGGTTGTTTCTGCGCGTGCGCGTAATAAACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGaaa                                                                                                                   >  1:822756/1‑140 (MQ=255)

                             tgcgcgtgcgcgTAATAAACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGa                                                                                                                     <  1:1354492/126‑1 (MQ=255)

                             tgcgcgtgcgcgTAATAAACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGa                                                                                                                     >  2:1354492/1‑126 (MQ=255)

                               cgcgtgcgcgtAATAAACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCa                                                                                                     >  1:593588/1‑140 (MQ=255)

                               cgcgtgcgcgtAATAAACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCa                                                                                                     <  1:1119653/140‑1 (MQ=255)

                                   tgcgcgtAATAAACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATgg                                                                                                 >  1:1384293/1‑140 (MQ=255)

                                     cgcgtAATAAACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGcc                                                                                               >  2:51000/1‑140 (MQ=255)

                                         taataaACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGGGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCgggc                                                                                           >  2:385589/1‑138 (MQ=255)

                                          aataaACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCt                                                                                          <  2:822051/140‑1 (MQ=255)

                                           ataaACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTg                                                                                         <  2:718671/140‑1 (MQ=255)

                                            taaACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGa                                                                                        <  1:237979/140‑1 (MQ=255)

                                                cGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGAtggt                                                                                    <  2:1333164/140‑1 (MQ=255)

                                                  tGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGGGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGAtggggg                                                                                  >  1:263585/1‑140 (MQ=255)

                                                  tGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGt                                                                                            >  1:639720/1‑130 (MQ=255)

                                                  tGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGt                                                                                            <  2:639720/130‑1 (MQ=255)

                                                    aTGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGaa                                                                                >  1:244412/1‑140 (MQ=255)

                                                    aTGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGaa                                                                                <  2:41971/140‑1 (MQ=255)

                                                    aTGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGaa                                                                                >  2:51084/1‑140 (MQ=255)

                                                    aTGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGaa                                                                                >  1:150074/1‑140 (MQ=255)

                                                    aTGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGa                                                                                 <  1:1296670/139‑1 (MQ=255)

                                                    aTGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGa                                                                                 >  2:1296670/1‑139 (MQ=255)

                                                         ggCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAatgat                                                                           >  1:479896/1‑140 (MQ=255)

                                                         ggCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAatgat                                                                           >  2:771088/1‑140 (MQ=255)

                                                                cGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCa                                                                    <  2:244412/140‑1 (MQ=255)

                                                                    acTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATggg                                                                <  1:1068661/140‑1 (MQ=255)

                                                                       gctgGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCg                                                             <  2:150074/140‑1 (MQ=255)

                                                                        ctgGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGa                                                            <  1:771088/140‑1 (MQ=255)

                                                                         tgGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGaa                                                           <  2:109730/140‑1 (MQ=255)

                                                                                tctctcCGCCCTGGGCGTTTACTTGCTGGGAAAGCTGCTTTCGCTGGGGGTGGGGGCACGTCCTGAACTGGCTGAAATTCGGAACCCCTCAATGGCCGGGCTGATGGGGGGAAAGATGGGGCCAAGGGGGGAAatcatca                                                    >  2:1254/1‑140 (MQ=255)

                                                                                      cGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTg                                              <  2:1384293/140‑1 (MQ=255)

                                                                                         tctGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCg                                           >  2:965233/1‑140 (MQ=255)

                                                                                         tctGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCg                                           >  1:457092/1‑140 (MQ=255)

                                                                                           tGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGt                                         >  2:1137430/1‑140 (MQ=255)

                                                                                            gggCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGGTGGGACCATGGGGCGAAATCATCATCGCTGCCGGtt                                        >  2:491503/1‑140 (MQ=255)

                                                                                            gggCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGtt                                        >  2:1087170/1‑140 (MQ=255)

                                                                                                        gCTGGTAACGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCg                            >  2:29680/1‑140 (MQ=255)

                                                                                                              aaCGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTgcg                      <  2:81024/140‑1 (MQ=255)

                                                                                                              aaCGCTGCTTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTgcg                      <  1:29680/140‑1 (MQ=255)

                                                                                                                 gctgctTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTgcggcg                   <  2:1359088/140‑1 (MQ=255)

                                                                                                                 gctgctTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTgcggcg                   <  2:593588/140‑1 (MQ=255)

                                                                                                                 gctgctTTCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTgcggcg                   <  1:1374973/140‑1 (MQ=255)

                                                                                                                      tttCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTAc              <  1:51084/140‑1 (MQ=255)

                                                                                                                       ttCGCTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACt             <  2:481863/140‑1 (MQ=255)

                                                                                                                           cTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAg         <  2:150027/140‑1 (MQ=255)

                                                                                                                           cTGGGTGTGGTGACACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAg         >  2:1105305/1‑140 (MQ=255)

                                                                                                                                  tggtgaCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGAcc  >  1:371506/1‑140 (MQ=255)

ATTGGTGTGGAAGGTGCCGTGGTTGTTTCTGCGCGTGCGCGTAATAAACGTGATGTTGGCAAAGCGACACTGCTGGCGGTTCTCTCCGCTCTGGGCGTTTACTTGCTGGTAACGCTGCTTTCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACC  >  NC_000913/1680190‑1680459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A