Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,681,079	T→C	V35A (GTC→GCC)	folM →	dihydromonapterin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,681,079	0	T	C	100.0%	199.8 / NA	56	V35A (GTC→GCC)	folM	dihydromonapterin reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/28); total (28/28)

ATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGTCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGCCGATGAAGTACTAAAAAGCACCCATGGTCT  >  NC_000913/1680945‑1681211

aTCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCt                                                                                                                                 <  2:289876/140‑1 (MQ=255)

     aTCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCgg                                                                                                                            <  1:20576/140‑1 (MQ=255)

         ttGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGTacac                                                                                                                        <  2:546900/140‑1 (MQ=255)

           ggATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGacacac                                                                                                                      <  1:717925/140‑1 (MQ=255)

                 cAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCa                                                                                                                >  1:175059/1‑140 (MQ=255)

                 cAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCa                                                                                                                >  2:386540/1‑140 (MQ=255)

                 cAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACAGGTGATTGCCAGCTATCGGACACACTATCCa                                                                                                                >  2:120131/1‑140 (MQ=255)

                   aaGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGc                                                                                                              <  1:562570/140‑1 (MQ=255)

                   aaGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGc                                                                                                              <  1:553310/140‑1 (MQ=255)

                    aGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGcc                                                                                                             >  1:707206/1‑140 (MQ=255)

                    aGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGAAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGcc                                                                                                             >  1:781832/1‑140 (MQ=255)

                       agaTAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCAtt                                                                                                          >  2:571951/1‑140 (MQ=255)

                        gaTAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTg                                                                                                         <  2:502445/140‑1 (MQ=255)

                           aaCGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATg                                                                                                      >  1:34704/1‑140 (MQ=255)

                                   gTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGAtt                                                                                              >  2:93065/1‑140 (MQ=255)

                                       agacaggCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATg                                                                                          <  1:611621/134‑1 (MQ=255)

                                          ccAGCCCTTGCCAATATTAATTACTGGCGAAGGTCGTCGCATCGGCCTCGCCCTCGCACGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAg                                                                                       <  2:781832/140‑1 (MQ=255)

                                            aGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGt                                                                                     <  2:34704/140‑1 (MQ=255)

                                             gCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTaa                                                                                            <  2:149071/132‑1 (MQ=255)

                                             gCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTaa                                                                                            >  1:149071/1‑132 (MQ=255)

                                              cccTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTgc                                                                                   <  1:386540/140‑1 (MQ=255)

                                              cccTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTgc                                                                                   <  2:222233/140‑1 (MQ=255)

                                              cccTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTgc                                                                                   <  2:107774/140‑1 (MQ=255)

                                              cccTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTgc                                                                                   <  1:496789/140‑1 (MQ=255)

                                                 ttGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCa                                                                                <  2:536046/140‑1 (MQ=255)

                                                   gCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAgt                                                                              >  2:607308/1‑140 (MQ=255)

                                                        tatTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTAtt                                                                         >  2:194699/1‑140 (MQ=255)

                                                        tatTAATTACTGGCGGAGGGCGTAGCATCGGCCTCCCCCTCGCCTGGCATTTCATTAATCAAAAACAAACGGAGATTGCCCGCTATCGGACACACCAACCAGCCATTGATGGACTGATTAATGCAAGTGCGAAgccagct                                                                         >  2:611621/1‑134 (MQ=255)

                                                             attaCTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGc                                                                    >  2:162605/1‑140 (MQ=255)

                                                             attaCTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGc                                                                    >  2:98909/1‑140 (MQ=255)

                                                              ttaCTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCt                                                                   >  2:779208/1‑140 (MQ=255)

                                                                  tGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGAtt                                                               >  2:194001/1‑140 (MQ=255)

                                                                  tGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGAtt                                                               >  1:51911/1‑140 (MQ=255)

                                                                  tGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGAtt                                                               >  2:713355/1‑140 (MQ=255)

                                                                    gCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGAtttt                                                             >  1:87058/1‑140 (MQ=255)

                                                                    gCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGAtttt                                                             >  1:381780/1‑140 (MQ=255)

                                                                     cGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGAttttt                                                            >  1:812001/1‑140 (MQ=255)

                                                                         ggTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGAc                                                        <  2:381780/140‑1 (MQ=255)

                                                                         ggTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGAc                                                        <  1:194699/140‑1 (MQ=255)

                                                                             gtcgCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAac                                                    <  1:607308/140‑1 (MQ=255)

                                                                                 cATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACAGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAacgacg                                                <  1:120131/140‑1 (MQ=255)

                                                                                  aTCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACgg                                               >  1:25227/1‑140 (MQ=255)

                                                                                    cGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCGGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACGgtg                                             >  2:828472/1‑140 (MQ=255)

                                                                                              ctcgcATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGtt                                   <  2:707206/140‑1 (MQ=255)

                                                                                              ctcgcATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGtt                                   <  1:571951/140‑1 (MQ=255)

                                                                                                 gcATGGCATTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGc                                <  1:80172/140‑1 (MQ=255)

                                                                                                        aTTTCATTAATCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGCCGATGaa                         <  2:51911/140‑1 (MQ=255)

                                                                                                                 aTCAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGCCGATGAAGTACTaaaa                >  2:826917/1‑140 (MQ=255)

                                                                                                                   cAAAAGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGCCGATGAAGTACTAAAAAg              <  1:194001/140‑1 (MQ=255)

                                                                                                                      aaGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGCCGATGAAGTACTaaa                 <  2:24399/134‑1 (MQ=255)

                                                                                                                      aaGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGCCGATGAAGTACTaaa                 >  1:24399/1‑134 (MQ=255)

                                                                                                                      aaGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGCCGATGAAGTACTAAAAAGCAc           >  2:66152/1‑140 (MQ=255)

                                                                                                                      aaGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGCCGATGAAGTACTAAAAAGCAc           >  1:223619/1‑140 (MQ=255)

                                                                                                                      aaGCAACCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGCCGATGAAGTACTAAAAAGCAc           >  2:863909/1‑140 (MQ=255)

                                                                                                                           aCCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGCCGATGAAGTACTAAAAAGCACCCATg      >  2:340332/1‑140 (MQ=255)

                                                                                                                           aCCGGTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGCCGATGAAGTACTAAAAAGCACCCATg      <  1:713355/140‑1 (MQ=255)

                                                                                                                               gTGATTGCCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGCCGATGAAGTACTAAAAAGCACCCATGGTCt  <  2:25227/140‑1 (MQ=255)

ATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGATTGTCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGTGTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGCCGATGAAGTACTAAAAAGCACCCATGGTCT  >  NC_000913/1680945‑1681211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A