Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,685,790	C→A	A267S (GCG→TCG)	fumC ←	fumarase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,685,790	0	C	A	100.0%	236.9 / NA	67	A267S (GCG→TCG)	fumC	fumarase C
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (22/45); total (22/45)

GGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTAC  >  NC_000913/1685654‑1685925

gggATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTc                                                                                                                                      >  2:687797/1‑140 (MQ=255)

         aTTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGtt                                                                                                                             >  1:432714/1‑140 (MQ=255)

            tCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCgg                                                                                                                          <  1:154158/140‑1 (MQ=255)

            tCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCgg                                                                                                                          >  2:577280/1‑140 (MQ=255)

              aCCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGgcg                                                                                                                        <  2:1073310/140‑1 (MQ=255)

              aCCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGgcg                                                                                                                        <  2:1072209/140‑1 (MQ=255)

              aCCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGgcg                                                                                                                        <  2:1055205/140‑1 (MQ=255)

              aCCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGgcg                                                                                                                        >  2:362577/1‑140 (MQ=255)

              aCCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGgcg                                                                                                                        <  2:454820/140‑1 (MQ=255)

              aCCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGgcg                                                                                                                        <  1:235221/140‑1 (MQ=255)

                cAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGcgcg                                                                                                                      <  2:1099056/140‑1 (MQ=255)

                cAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGcgcg                                                                                                                      <  2:619616/140‑1 (MQ=255)

                      cGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACa                                                                                                                >  1:23415/1‑140 (MQ=255)

                        cAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACaaa                                                                                                              <  2:560834/140‑1 (MQ=255)

                        cAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACaaa                                                                                                              <  2:513927/140‑1 (MQ=255)

                           cgcgGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACgg                                                                                                           <  1:124315/140‑1 (MQ=255)

                            gcgGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGt                                                                                                          <  2:435872/140‑1 (MQ=255)

                             cgGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTg                                                                                                         >  2:910306/1‑140 (MQ=255)

                               ggCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGca                                                                                                       <  1:558363/140‑1 (MQ=255)

                               ggCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGca                                                                                                       <  2:272988/140‑1 (MQ=255)

                                gCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGcac                                                                                                      >  1:912457/1‑140 (MQ=255)

                                   agagGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAgg                                                                                                   <  2:945159/140‑1 (MQ=255)

                                   agagGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAgg                                                                                                   <  2:945160/140‑1 (MQ=255)

                                             cagcagACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGc                                                                                         <  2:1300136/140‑1 (MQ=255)

                                              agcGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGcc                                                                                        <  1:638875/140‑1 (MQ=255)

                                               gcGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGc                                                                                         >  2:730125/1‑138 (MQ=255)

                                               gcGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGc                                                                                         <  1:730125/138‑1 (MQ=255)

                                                   aCATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTc                                                                                   <  1:202528/140‑1 (MQ=255)

                                                     atcatTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCAt                                                                                 <  2:869596/140‑1 (MQ=255)

                                                      tcatTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATc                                                                                >  1:1137201/1‑140 (MQ=255)

                                                      tcatTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATc                                                                                >  1:940589/1‑140 (MQ=255)

                                                          tGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCt                                                                            >  2:554843/1‑140 (MQ=255)

                                                              gATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgc                                                                        <  2:1401166/140‑1 (MQ=255)

                                                                ttttCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcga                                                                      <  2:940589/140‑1 (MQ=255)

                                                                    catcaGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcgacgcg                                                                  <  1:101938/140‑1 (MQ=255)

                                                                    catcaGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcgacgcg                                                                  <  2:423721/140‑1 (MQ=255)

                                                                      tcaGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCa                                                                <  2:741458/140‑1 (MQ=255)

                                                                          tGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACt                                                            <  2:1053513/140‑1 (MQ=255)

                                                                            aCGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTcc                                                          >  2:1412445/1‑140 (MQ=255)

                                                                             cGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcgacgc                                                                   <  1:1345057/130‑1 (MQ=255)

                                                                             cGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcgacgc                                                                   >  2:1345057/1‑130 (MQ=255)

                                                                               cAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGa                                                       <  2:239679/140‑1 (MQ=255)

                                                                                  ccAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATgg                                                    >  2:1358967/1‑140 (MQ=255)

                                                                                    aaCCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCAAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGt                                                  >  2:663255/1‑140 (MQ=255)

                                                                                       ccTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTAtt                                               <  2:1137201/140‑1 (MQ=255)

                                                                                       ccTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTAtt                                               <  2:8801/140‑1 (MQ=255)

                                                                                       ccTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTAtt                                               <  1:194494/140‑1 (MQ=255)

                                                                                       ccTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTAtt                                               <  1:362577/140‑1 (MQ=255)

                                                                                         tttCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTa                                             <  2:1404870/140‑1 (MQ=255)

                                                                                           tCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGt                                           >  1:857247/1‑140 (MQ=255)

                                                                                           tCAACGCGCCGTGCGCCTGAACCA‑GGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTc                                          <  1:463750/140‑1 (MQ=255)

                                                                                            cAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTc                                          >  1:269668/1‑140 (MQ=255)

                                                                                                 cgcCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTa                                     <  1:22990/140‑1 (MQ=255)

                                                                                                 cgcCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTa                                     >  2:22990/1‑140 (MQ=255)

                                                                                                 cgcCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAAAAAACGGTGCCCGGGAAATGCCTGCCGGTTCATCTGCTACGCGCCCCGCATACCCGGGATGGGAATTTAGTCCATTa                                     >  2:670395/1‑140 (MQ=255)

                                                                                                   ccGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTAcc                                   <  1:496618/140‑1 (MQ=255)

                                                                                                      tGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCAc                                <  1:577280/140‑1 (MQ=255)

                                                                                                           cTGAACCAGGGCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTg                           <  1:472876/140‑1 (MQ=255)

                                                                                                                   gggCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCa                   >  2:181996/1‑140 (MQ=255)

                                                                                                                   gggCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCa                   <  1:1412445/140‑1 (MQ=255)

                                                                                                                   gggCATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCa                   <  2:912457/140‑1 (MQ=255)

                                                                                                                      cATCACAGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAaga                <  2:23415/140‑1 (MQ=255)

                                                                                                                         cacaGGTCGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGcc             >  2:688900/1‑140 (MQ=255)

                                                                                                                            aGGTCGCAAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGt          <  1:663255/140‑1 (MQ=255)

                                                                                                                                cGCCAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCg      <  1:910306/140‑1 (MQ=255)

                                                                                                                                   cAGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTa   >  2:7689/1‑140 (MQ=255)

                                                                                                                                    aGCGATTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTAc  <  2:857247/140‑1 (MQ=255)

GGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTAC  >  NC_000913/1685654‑1685925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A