Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,769,193	G→A	W40* (TGG→TGA)	ydiK →	putative transporter YdiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,769,193	0	G	A	100.0%	169.0 / NA	51	W40* (TGG→TGA)	ydiK	putative transporter YdiK
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/25); total (26/25)

GAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTG  >  NC_000913/1769063‑1769328

gAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACg                                                                                                                                <  1:1045567/140‑1 (MQ=255)

        gtTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTAt                                                                                                                        >  2:382053/1‑140 (MQ=255)

                   tGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGc                                                                                                             <  1:382053/140‑1 (MQ=255)

                   tGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGc                                                                                                             >  1:176680/1‑140 (MQ=255)

                   tGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGc                                                                                                             <  1:137875/140‑1 (MQ=255)

                      tCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCgg                                                                                                          <  1:119452/140‑1 (MQ=255)

                       cGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCggg                                                                                                         >  1:379816/1‑139 (MQ=255)

                       cGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGt                                                                                                         >  1:185905/1‑140 (MQ=255)

                        gTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTa                                                                                                        >  1:672389/1‑140 (MQ=255)

                        gTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTa                                                                                                        >  2:532213/1‑140 (MQ=255)

                         tCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTAt                                                                                                       >  2:1171825/1‑140 (MQ=255)

                                     tGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCgg                                                                                                          >  1:1160374/1‑125 (MQ=255)

                                     tGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCgg                                                                                                          <  2:1160374/125‑1 (MQ=255)

                                       tCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCaa                                                                                         <  1:1111657/140‑1 (MQ=255)

                                             aaaTTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATc                                                                                   >  2:1175554/1‑140 (MQ=255)

                                                 tCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGt                                                                                               >  1:197977/1‑124 (MQ=255)

                                                 tCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGt                                                                                               <  2:197977/124‑1 (MQ=255)

                                                         cGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGgccgc                                                                       <  2:602978/140‑1 (MQ=255)

                                                              cTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCACGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGctc                                                                  <  2:873185/140‑1 (MQ=255)

                                                                   tttAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCg                                                             <  1:1171825/140‑1 (MQ=255)

                                                                      aGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCg                                                          >  1:1008619/1‑140 (MQ=255)

                                                                           tcatGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTg                                                     >  2:909347/1‑140 (MQ=255)

                                                                                  tgtgGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCg                                                             >  2:927634/1‑125 (MQ=255)

                                                                                  tgtgGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCg                                                             <  1:927634/125‑1 (MQ=255)

                                                                                   gtgGCATTTCTGTGACTTGTCCAACCCTTTATTCTCGGCTTTGGATGAGCAGGTTCGGTGTTTATCGCCACCTGTCGCGTATTTTTCCGTTTGCAAAAGCTCATGTTTGGCCGCCGCACTCTCGCCGTTCTGGtgatggc                                             <  2:786186/140‑3 (MQ=255)

                                                                                   gtgGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGAc                                             <  2:394401/140‑1 (MQ=255)

                                                                                    tgGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACg                                            <  1:1103202/140‑1 (MQ=255)

                                                                                    tgGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACg                                            <  1:909347/140‑1 (MQ=255)

                                                                                    tgGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACg                                            >  1:1206112/1‑140 (MQ=255)

                                                                                    tgGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACg                                            <  2:1195082/140‑1 (MQ=255)

                                                                                     ggCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGc                                           >  1:129981/1‑140 (MQ=255)

                                                                                      gCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCt                                          <  2:672389/140‑1 (MQ=255)

                                                                                          gTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGGATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGttt                                      >  1:795598/1‑139 (MQ=255)

                                                                                           tCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGttat                                     >  1:859339/1‑140 (MQ=255)

                                                                                                ggATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTg                                >  1:158930/1‑140 (MQ=255)

                                                                                                 gATTGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGa                               <  2:1206112/140‑1 (MQ=255)

                                                                                                    tGTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATgg                            >  1:349367/1‑140 (MQ=255)

                                                                                                     gTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGgt                           >  1:724161/1‑140 (MQ=255)

                                                                                                     gTTCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGgt                           <  2:129981/140‑1 (MQ=255)

                                                                                                      ttCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGgtg                          <  2:176680/140‑1 (MQ=255)

                                                                                                      ttCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGgtg                          >  1:437603/1‑140 (MQ=255)

                                                                                                      ttCAACCCTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGgtg                          >  1:693593/1‑140 (MQ=255)

                                                                                                            ccTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTatc                    <  1:532213/140‑1 (MQ=255)

                                                                                                            ccTTTATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTatc                    <  1:1175554/140‑1 (MQ=255)

                                                                                                              tttATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTatcat                  <  2:185905/140‑1 (MQ=255)

                                                                                                                tATTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATcc                >  1:1396470/1‑140 (MQ=255)

                                                                                                                 aTTCTCGGCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATccc               <  2:1396470/140‑1 (MQ=255)

                                                                                                                       ggCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTAttgc         >  1:450931/1‑140 (MQ=255)

                                                                                                                        gCTTTGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGAATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCCCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTAttgct        >  2:1295695/1‑140 (MQ=255)

                                                                                                                           ttGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTAttgctttg     <  2:795598/140‑1 (MQ=255)

                                                                                                                            tGCATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTAttgctttgc    >  1:314234/1‑140 (MQ=255)

                                                                                                                              cATGAGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTg  <  2:1008619/140‑1 (MQ=255)

GAAAAGTAGTTATGGTAAATGTTCGTCAGCCCAGGGATGTCGCACAAATTCTGCTTTCGGTGCTGTTTTTAGCCATCATGATTGTGGCATGTCTGTGGATTGTTCAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTG  >  NC_000913/1769063‑1769328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A