Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 191 83.5 2830284 99.3% 2810472 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,576,004 (T)8→7 intergenic (‑199/+94) eutS ← / ← maeB putative structural protein, ethanolamine utilization microcompartment/malate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,575,9970T.100.0% 167.0 / NA 39intergenic (‑192/+101)eutS/maeBputative structural protein, ethanolamine utilization microcompartment/malate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (20/19);  total (20/19)

AAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGTTTGCGCTTCTACCACGGCCA  >  NC_000913/2575866‑2576132
                                                                                                                                   |                                                                                                                                       
aaaaTTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCtttttttat                                                                                                                                 >  2:276020/1‑138 (MQ=255)
aaaaTTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCtttttttat                                                                                                                                 <  1:881160/140‑3 (MQ=255)
  aaTTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCtttttttatta                                                                                                                               >  2:113011/1‑136 (MQ=255)
                    aaaaGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGtaata                                                                                                            <  2:473713/140‑1 (MQ=255)
                         gTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCtt                                                                                                       <  1:276020/140‑1 (MQ=255)
                         gTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCtt                                                                                                       <  1:113011/140‑1 (MQ=255)
                            aGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACt                                                                                                                    >  2:540669/1‑124 (MQ=255)
                            aGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACt                                                                                                                    <  1:540669/124‑1 (MQ=255)
                            aGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTAc                                                                                                    <  1:451395/140‑1 (MQ=255)
                               tAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTaa                                                                                                 <  2:1044015/140‑1 (MQ=255)
                                  aTCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGtaat                                                                                                             <  1:1323367/125‑1 (MQ=255)
                                  aTCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGtaat                                                                                                             >  2:1323367/1‑125 (MQ=255)
                                   tCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAatata                                                                                             >  1:175281/1‑140 (MQ=255)
                                      cTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTg                                                                                          >  2:86822/1‑140 (MQ=255)
                                       tAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAatatat                                                                                            <  2:614078/137‑1 (MQ=255)
                                       tAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAatatat                                                                                            >  1:614078/1‑137 (MQ=255)
                                       tAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGc                                                                                         >  1:1207370/1‑140 (MQ=255)
                                        aaGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCa                                                                                        >  2:871154/1‑140 (MQ=255)
                                         aGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAg                                                                                       <  2:1207370/140‑1 (MQ=255)
                                             tGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTaa                                                                                   <  1:86822/140‑1 (MQ=255)
                                                      atTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTAc                                                                          <  1:871154/140‑1 (MQ=255)
                                                      atTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTAc                                                                          <  2:175281/140‑1 (MQ=255)
                                                         cGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCt                                                                       >  2:260467/1‑140 (MQ=255)
                                                          gCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCTg                                                                      <  1:260467/140‑1 (MQ=255)
                                                            tgtgGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGaa                                                                    >  2:1396790/1‑140 (MQ=255)
                                                              tggtgCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTAc                                                                          >  2:1191629/1‑132 (MQ=255)
                                                              tggtgCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTAc                                                                          <  1:1191629/132‑1 (MQ=255)
                                                              tggtgCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAata                                                                  <  2:417669/140‑1 (MQ=255)
                                                                gtgCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTAc                                                                          <  2:784454/130‑1 (MQ=255)
                                                                gtgCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTAc                                                                          >  1:784454/1‑130 (MQ=255)
                                                                   cATAAACTTTCATATGCAACGCAAGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAg                                                             >  2:742317/1‑140 (MQ=255)
                                                                              atatGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTga                                                  <  1:1396790/140‑1 (MQ=255)
                                                                                      cGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTaaa                                          >  2:1180205/1‑140 (MQ=255)
                                                                                      cGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTaaa                                          >  1:231889/1‑140 (MQ=255)
                                                                                        cATGAATATAAATAAAATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTaaaaa                                        >  2:143576/1‑140 (MQ=255)
                                                                                                taaataaaATAATATCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACa                                <  2:1168884/140‑1 (MQ=255)
                                                                                                              tCGAGATATTATATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGTTTgc                  <  1:143576/140‑1 (MQ=255)
                                                                                                                      ttatATAGACTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGTTTGCGCTTCTAc          >  1:976685/1‑140 (MQ=255)
                                                                                                                              aCTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGTTTGCGCTTCTACCACGGCCa  >  1:181175/1‑140 (MQ=255)
                                                                                                                              aCTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGTTTGCGCTTCTACCACGGCCa  >  1:241747/1‑140 (MQ=255)
                                                                                                                              aCTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGTTTGCGCTTCTACCACGGCCa  >  1:76667/1‑140 (MQ=255)
                                                                                                                              aCTGC‑TTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGTTTGCGCTTCTACCACGGCCa  >  1:1111183/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                       
AAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGTTTGCGCTTCTACCACGGCCA  >  NC_000913/2575866‑2576132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A