Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	183	98.6	3427438	96.9%	3321187	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,577,767	T→C	N204S (AAC→AGC)	maeB ←	malate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,577,767	0	T	C	100.0%	256.1 / NA	70	N204S (AAC→AGC)	maeB	malate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (40/30); total (40/30)

GGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGG  >  NC_000913/2577631‑2577901

ggTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCa                                                                                                                                     >  2:773323/1‑140 (MQ=255)

 gTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCAt                                                                                                                                    >  2:1572756/1‑140 (MQ=255)

         gCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGa                                                                                                                            >  2:1455783/1‑140 (MQ=255)

          ccGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCTTGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGAt                                                                                                                           >  2:638072/1‑140 (MQ=255)

           cGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGAtt                                                                                                                          <  2:1436961/140‑1 (MQ=255)

                         gCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATGGCTGCGGCACCggc                                                                                                            >  2:1050674/1‑140 (MQ=255)

                         gCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACACCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCcgc                                                                                                            >  2:1115396/1‑140 (MQ=255)

                          cATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCcgcg                                                                                                           >  2:913955/1‑140 (MQ=255)

                          cATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCcgcg                                                                                                           >  1:308685/1‑140 (MQ=255)

                          cATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCcgcg                                                                                                           <  1:1572756/140‑1 (MQ=255)

                            tatGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGcc                                                                                                         <  1:1455783/140‑1 (MQ=255)

                            tatGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGcc                                                                                                         <  1:204308/140‑1 (MQ=255)

                             atGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAGTCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCg                                                                                                        >  1:1163373/1‑140 (MQ=255)

                               gCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCAGCCGCGCCGGa                                                                                                      >  2:1053416/1‑140 (MQ=255)

                               gCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCCTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGa                                                                                                      >  2:463116/1‑140 (MQ=255)

                                   cTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAacc                                                                                                  >  1:973872/1‑140 (MQ=255)

                                   cTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAacc                                                                                                  <  1:913955/140‑1 (MQ=255)

                                      tGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAaccacc                                                                                               >  1:850196/1‑140 (MQ=255)

                                      tGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAaccacc                                                                                               >  1:1288379/1‑140 (MQ=255)

                                           tCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCg                                                                                          >  2:909925/1‑140 (MQ=255)

                                           tCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCg                                                                                          <  2:1489403/140‑1 (MQ=255)

                                                  tGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCg                                                                                   >  2:745208/1‑140 (MQ=255)

                                                   gTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCgg                                                                                  <  1:501288/140‑1 (MQ=255)

                                                   gTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCgg                                                                                  >  1:652634/1‑140 (MQ=255)

                                                     ttGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGgag                                                                                <  2:850196/140‑1 (MQ=255)

                                                           cACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGttt                                                                          <  1:1053416/140‑1 (MQ=255)

                                                            aCGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGtttt                                                                         >  2:649912/1‑140 (MQ=255)

                                                              ggCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTtc                                                                       >  2:462750/1‑140 (MQ=255)

                                                              ggCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTtc                                                                       >  1:1227269/1‑140 (MQ=255)

                                                                    gatagataACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTccacc                                                                 <  1:909925/140‑1 (MQ=255)

                                                                          taACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACgcgc                                                           >  1:550453/1‑140 (MQ=255)

                                                                          taACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACgcgc                                                           >  1:17743/1‑140 (MQ=255)

                                                                          taACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACgcgc                                                           >  1:966224/1‑140 (MQ=255)

                                                                           aaCGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCa                                                          <  2:1191940/140‑1 (MQ=255)

                                                                           aaCGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCa                                                          <  2:308685/140‑1 (MQ=255)

                                                                           aaCGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCa                                                          <  1:1064586/140‑1 (MQ=255)

                                                                           aaCGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCa                                                          <  1:462750/140‑1 (MQ=255)

                                                                             cGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCGCCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAg                                                        <  2:1227269/140‑1 (MQ=255)

                                                                             cGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAg                                                        <  2:986934/140‑1 (MQ=255)

                                                                             cGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAg                                                        >  1:986934/1‑140 (MQ=255)

                                                                               ccTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCa                                                          <  1:1376937/136‑1 (MQ=255)

                                                                               ccTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCa                                                          >  2:1376937/1‑136 (MQ=255)

                                                                               ccTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGcc                                                      >  1:1537468/1‑140 (MQ=255)

                                                                                cTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCg                                                     <  1:1443870/140‑1 (MQ=255)

                                                                                  tttGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGtt                                                   >  2:640371/1‑140 (MQ=255)

                                                                                     gAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAg                                                <  2:973872/140‑1 (MQ=255)

                                                                                           cAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATggc                                          >  2:1445003/1‑140 (MQ=255)

                                                                                             aaCCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCTTCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATggcgg                                        <  2:475178/140‑1 (MQ=255)

                                                                                               ccACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCa                                      <  2:966224/140‑1 (MQ=255)

                                                                                                 aCGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGt                                    <  1:808857/140‑1 (MQ=255)

                                                                                                      gttatgttTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGa                               >  2:671053/1‑140 (MQ=255)

                                                                                                      gttatgttTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGa                               >  2:516245/1‑140 (MQ=255)

                                                                                                        tatgttTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATa                             >  2:1405872/1‑140 (MQ=255)

                                                                                                         atgttTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATaa                            >  2:1239215/1‑140 (MQ=255)

                                                                                                          tgttTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAAt                           >  1:628655/1‑140 (MQ=255)

                                                                                                          tgttTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAAt                           >  2:482224/1‑140 (MQ=255)

                                                                                                          tgttTTTGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTCCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAAt                           >  1:447478/1‑140 (MQ=255)

                                                                                                            tttttGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATtg                         <  1:745208/140‑1 (MQ=255)

                                                                                                              tttGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATtgcc                       <  1:640371/140‑1 (MQ=255)

                                                                                                              tttGCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTCCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATtgcc                       <  2:447478/140‑1 (MQ=255)

                                                                                                                 gCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATtgccgtg                    <  1:671053/140‑1 (MQ=255)

                                                                                                                 gCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATtgccgtg                    <  1:516245/140‑1 (MQ=255)

                                                                                                                 gCAGACCCAGCGCTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATtgccgtg                    >  1:1442265/1‑140 (MQ=255)

                                                                                                                          gcgcTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGa           <  2:1288379/140‑1 (MQ=255)

                                                                                                                           cgcTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGAt          >  1:937717/1‑140 (MQ=255)

                                                                                                                           cgcTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGAt          <  1:1405872/140‑1 (MQ=255)

                                                                                                                           cgcTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGAt          <  2:652634/140‑1 (MQ=255)

                                                                                                                            gcTACCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGAtc         >  2:551225/1‑140 (MQ=255)

                                                                                                                               aCCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGAtcgtc      >  1:153323/1‑140 (MQ=255)

                                                                                                                               aCCAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGAtcgtc      >  1:1703241/1‑140 (MQ=255)

                                                                                                                                ccAGCAGGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGAtcgtcg     >  2:193056/1‑140 (MQ=255)

                                                                                                                                   gcagGCTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTgg  >  2:74166/1‑140 (MQ=255)

GGTACGTTTGCCGTCATCCACCACCGCATATGCGGCTTTGGTTTCCGCCATGTTTGGCTCACGGCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGG  >  NC_000913/2577631‑2577901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A