Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,964,353	T→C	intergenic (‑176/+8)	ppdA ← / ← thyA	conserved protein PpdA/thymidylate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,964,353	0	T	C	100.0%	179.4 / NA	51	intergenic (‑176/+8)	ppdA/thyA	conserved protein PpdA/thymidylate synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/25); total (26/25)

AGTTTCTATTTCTTCGGCGCATCTTCCGGACTATTTACGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGTTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGGACGCGGTT  >  NC_000913/2964217‑2964488

aGTTTCTATTTCTTCGGCGCATCTTCCGGACTATTTACGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGCAAAAAAACCGACGCACACTGGCGTACGCTCTGGCAGGATGCTTc                                                                                                                                      >  2:1293321/1‑140 (MQ=255)

       aTTTCTTCGGCGCATCTTCCGGACTATTTACGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTa                                                                                                                               >  2:353650/1‑140 (MQ=255)

        tttCTTCGGCGCATCTTCCGGACTATTTACGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAg                                                                                                                              <  1:77782/140‑1 (MQ=255)

          tcttcGGCGCATCTTCCGGACTATTTACGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGT‑AAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATa                                                                                                                           <  1:550035/140‑1 (MQ=255)

            ttcGGCGCATCTTCCGGACTATTTACGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAg                                                                                                                          >  2:42255/1‑140 (MQ=255)

             tcGGCGCATCTTCCGGACTATTTACGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGc                                                                                                                         >  1:65894/1‑140 (MQ=255)

              cGGCGCATCTTCCGGACTATTTACGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGcc                                                                                                                        >  1:710142/1‑140 (MQ=255)

                gcgcATCTTCCGGACTATTTACGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCAc                                                                                                                      >  2:407050/1‑140 (MQ=255)

                   cATCTTCCGGACTATTTACGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCgg                                                                                                                   <  2:989267/140‑1 (MQ=255)

                      cTTCCGGACTATTTACGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGgcgc                                                                                                                <  1:353650/140‑1 (MQ=255)

                          cGGACTATTTACGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTa                                                                                                            <  2:1130110/140‑1 (MQ=255)

                             aCTATTTACGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATg                                                                                                         <  2:65894/140‑1 (MQ=255)

                              cTATTTACGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGc                                                                                                        >  1:854118/1‑140 (MQ=255)

                               tATTTACGCCGTTGCAGGACGTTGCAAAAATTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGcc                                                                                                       <  2:1322517/140‑1 (MQ=255)

                                 tttACGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACG‑‑CACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGa                                                                                                   >  1:29886/1‑140 (MQ=255)

                                  ttACGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCgg                                                                                                    >  2:352552/1‑140 (MQ=255)

                                     cGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATg                                                                                                 <  2:402980/140‑1 (MQ=255)

                                      gCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGc                                                                                                >  2:507087/1‑140 (MQ=255)

                                           tGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGTTAGCCACCGGCGCTTTAATGCCCGGATGCGGATc                                                                                           >  2:299168/1‑140 (MQ=255)

                                           tGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATc                                                                                           >  1:717985/1‑140 (MQ=255)

                                                    ttGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTc                                                                                  >  2:327436/1‑140 (MQ=255)

                                                     tGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTc                                                                                  >  1:553191/1‑139 (MQ=255)

                                                     tGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTc                                                                                  <  2:553191/139‑1 (MQ=255)

                                                     tGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCa                                                                                 >  2:420474/1‑140 (MQ=255)

                                                     tGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCa                                                                                 >  2:931882/1‑140 (MQ=255)

                                                       cAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAAt                                                                               >  1:188229/1‑140 (MQ=255)

                                                       cAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAAt                                                                               >  2:996955/1‑140 (MQ=255)

                                                                gggAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTc                                                                      <  1:252630/140‑1 (MQ=255)

                                                                     ggCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGa                                                                 <  1:1144676/140‑1 (MQ=255)

                                                                      gCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGaa                                                                >  1:562268/1‑140 (MQ=255)

                                                                       cGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGaaa                                                               <  2:622734/140‑1 (MQ=255)

                                                                         tctcGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACg                                                             <  1:407050/140‑1 (MQ=255)

                                                                          ctcGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACgg                                                            <  1:931882/140‑1 (MQ=255)

                                                                              aagaaTTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGt                                                        <  1:778234/140‑1 (MQ=255)

                                                                                gaaTTTAACGGAGGGTAAAAAAACCGGCGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCg                                                      <  1:1293321/140‑1 (MQ=255)

                                                                                gaaTTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCg                                                      <  1:42255/140‑1 (MQ=255)

                                                                                     tAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGAt                                                 <  2:18657/140‑1 (MQ=255)

                                                                                             ggTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCgg                                         >  2:469598/1‑140 (MQ=255)

                                                                                             ggTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCgg                                         >  2:1061637/1‑140 (MQ=255)

                                                                                              gTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCggg                                        >  2:362376/1‑140 (MQ=255)

                                                                                               tAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGt                                       <  1:507087/140‑1 (MQ=255)

                                                                                               tAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGt                                       <  1:420474/140‑1 (MQ=255)

                                                                                                aaaaaaaCCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGtt                                      <  1:1061637/140‑1 (MQ=255)

                                                                                                  aaaaaCCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTa                                    <  1:469598/140‑1 (MQ=255)

                                                                                                      aCCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGtt                                <  1:327436/140‑1 (MQ=255)

                                                                                                      aCCGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGtt                                <  2:25516/140‑1 (MQ=255)

                                                                                                       ccGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGttt                               >  1:789765/1‑140 (MQ=255)

                                                                                                       ccGACGCACACTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGttt                               >  1:871783/1‑140 (MQ=255)

                                                                                                            acgcacTGGCGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATaa                          <  2:29886/137‑1 (MQ=255)

                                                                                                                     cGTCGGCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCg                 >  1:811776/1‑140 (MQ=255)

                                                                                                                          gCTCTGGCAGGATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGc            >  2:955494/1‑140 (MQ=255)

                                                                                                                                   ggATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGGACGCGGt   >  2:736223/1‑140 (MQ=255)

                                                                                                                                    gATGCTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGGACGCGGtt  >  2:291280/1‑140 (MQ=255)

AGTTTCTATTTCTTCGGCGCATCTTCCGGACTATTTACGCCGTTGCAGGACGTTGCAAAATTTCGGGAAGGCGTCTCGAAGAATTTAACGGAGGGTAAAAAAACCGACGCACACTGGCGTCGGCTCTGGCAGGATGTTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGGACGCGGTT  >  NC_000913/2964217‑2964488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A