Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,975,047	T→C	E323G (GAG→GGG)	aas ←	acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,975,047	0	T	C	100.0%	200.1 / NA	57	E323G (GAG→GGG)	aas	acyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (33/24); total (33/24)

CGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACG  >  NC_000913/2974911‑2975183

cgcCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCg                                                                                                                                       >  2:1064408/1‑140 (MQ=255)

  ccTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGc                                                                                                                                     <  2:998873/140‑1 (MQ=255)

    tcttctTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAg                                                                                                                                   >  2:693364/1‑140 (MQ=255)

              gctgctGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGt                                                                                                                         >  1:1376884/1‑140 (MQ=255)

                    gTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCt                                                                                                                   <  1:1054257/140‑1 (MQ=255)

                             gtgCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCgaga                                                                                                          <  1:647209/140‑1 (MQ=255)

                              tgCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGtt                                                                                                                        <  2:75179/125‑1 (MQ=255)

                              tgCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGtt                                                                                                                        >  1:75179/1‑125 (MQ=255)

                               gCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTCTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGaaa                                                                                                        >  1:24447/1‑140 (MQ=255)

                                    aCGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGc                                                                                                   <  1:424584/140‑1 (MQ=255)

                                     cgcgGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCg                                                                                                  <  2:827507/140‑1 (MQ=255)

                                        ggCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGa                                                                                               >  1:680262/1‑140 (MQ=255)

                                               gCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGTGGGAAGTGAAg                                                                                        <  2:1217577/140‑1 (MQ=255)

                                                      aGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATATACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGTAGATGGttt                                                                                 >  1:1300331/1‑140 (MQ=255)

                                                        cGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTTAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTg                                                                               >  1:339585/1‑140 (MQ=255)

                                                        cGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTg                                                                               >  2:754920/1‑140 (MQ=255)

                                                        cGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTg                                                                               >  2:83372/1‑140 (MQ=255)

                                                               ccATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAg                                                                        >  2:35088/1‑140 (MQ=255)

                                                                cATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTTAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAgc                                                                       <  2:339585/140‑1 (MQ=255)

                                                                 aTACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAgcc                                                                      >  1:33871/1‑140 (MQ=255)

                                                                   aCTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAgccgc                                                                    <  2:1376884/140‑1 (MQ=255)

                                                                   aCTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAgccgc                                                                    <  2:47781/140‑1 (MQ=255)

                                                                   aCTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAgccgc                                                                    <  1:83372/140‑1 (MQ=255)

                                                                   aCTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAgccgc                                                                    <  1:8184/140‑1 (MQ=255)

                                                                   aCTTTGTCGGCAGTGGTGACATCTGCTCTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAgccgc                                                                    <  2:24447/140‑1 (MQ=255)

                                                                       tGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAgc                                                                       >  1:175410/1‑133 (MQ=255)

                                                                       tGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAgc                                                                       <  2:175410/133‑1 (MQ=255)

                                                                       tGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTa                                                                >  1:33848/1‑140 (MQ=255)

                                                                       tGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTa                                                                >  1:61708/1‑140 (MQ=255)

                                                                         tCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAAt                                                              >  2:128202/1‑140 (MQ=255)

                                                                          cGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATa                                                             >  1:695499/1‑140 (MQ=255)

                                                                             cAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCa                                                          <  1:693364/140‑1 (MQ=255)

                                                                             cAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCa                                                          <  2:221486/140‑1 (MQ=255)

                                                                                     aCATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAg                                                  <  1:1311145/140‑1 (MQ=255)

                                                                                         cTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCt                                              <  2:33871/140‑1 (MQ=255)

                                                                                          tGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCtt                                             >  2:295004/1‑140 (MQ=255)

                                                                                            cTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCtttt                                           >  1:160325/1‑140 (MQ=255)

                                                                                            cTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCtttt                                           >  2:634472/1‑140 (MQ=255)

                                                                                                 aaaTCTTCCAGATACACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGCAGCAGGGTTTGATTTCAACCGCCGTAATAGCACTGGTCAGCCCTTTTTcccc                                      >  2:1045734/1‑140 (MQ=255)

                                                                                                   aTCTTCCAGATAGACCCAGCGCCCCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGAGGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCgg                                    <  1:533182/140‑1 (MQ=255)

                                                                                                   aTCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCgg                                    <  2:504576/140‑1 (MQ=255)

                                                                                                      ttCCAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAg                                 <  1:1064408/140‑1 (MQ=255)

                                                                                                        ccAGATAGACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAgtg                               <  2:61708/140‑1 (MQ=255)

                                                                                                              ggccccAGCGCACCTGTGTAAGTGACCCCGGCAAATGCCAGATTTTCCTTTTAGCGAAAAACGGGCGAGAAGAAAAGTGGGTTTTGTTTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTtc                         <  2:1300331/137‑1 (MQ=255)

                                                                                                               gACCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTtca                        >  1:348418/1‑140 (MQ=255)

                                                                                                                aCCCAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTtcat                       >  1:617081/1‑140 (MQ=255)

                                                                                                                 cccAGCGCACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTtcatc                      >  1:313334/1‑140 (MQ=255)

                                                                                                                     gcgcACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCGCTTTTACCCCGGCAGTGTAGTTCATCATTg                  >  2:866843/1‑140 (MQ=255)

                                                                                                                      cgcACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGc                 >  2:314363/1‑140 (MQ=255)

                                                                                                                      cgcACCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGGGAAGATGGTTTTGATTTCAGCCCCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGc                 >  2:967654/1‑140 (MQ=255)

                                                                                                                         aCCTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTTAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCCGGGCAGTTCATCATTTCggg              >  2:558648/1‑140 (MQ=255)

                                                                                                                           cTGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCa            >  1:198244/1‑140 (MQ=255)

                                                                                                                            tGAGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCAt           >  2:1312655/1‑140 (MQ=255)

                                                                                                                              aGTAAGTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATgc         >  1:231562/1‑140 (MQ=255)

                                                                                                                                   gTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATgcg        >  2:1133686/1‑136 (MQ=255)

                                                                                                                                   gTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATgcg        <  1:1133686/136‑1 (MQ=255)

                                                                                                                                   gTTGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGa    <  1:634472/140‑1 (MQ=255)

                                                                                                                                     tGCCCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGAGAAGAAGGTTTTGAGTTCAGCCGCCGCAATAGCACTGGTCAGCCCCTTTACCCCGGCAGTGTAGTTCATCATTGGGGGCATGGGgcggcg  >  2:1313870/1‑140 (MQ=255)

CGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACG  >  NC_000913/2974911‑2975183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A