Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 191 83.5 2830284 99.3% 2810472 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,065,625 Δ1 bp coding (824/1479 nt) scpC → propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,065,6220A.100.0% 200.9 / NA 47coding (821/1479 nt)scpCpropionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (26/21);  total (26/21)

CCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATT  >  NC_000913/3065483‑3065758
                                                                                                                                           |                                                                                                                                        
cccATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGa                                                                                                                                          <  1:997338/140‑1 (MQ=255)
cccATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGa                                                                                                                                          <  1:730415/140‑1 (MQ=255)
cccATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGa                                                                                                                                          <  1:121662/140‑1 (MQ=255)
    tgtgCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGaaacc                                                                                                                                      >  2:182229/1‑138 (MQ=255)
           gcagATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGaaa                                                                                                                                        >  2:1293248/1‑131 (MQ=255)
           gcagATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGaaa                                                                                                                                        <  1:1293248/131‑1 (MQ=255)
              gATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATtcc                                                                                                                           <  1:1102868/140‑1 (MQ=255)
              gATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATtcc                                                                                                                           <  2:558125/140‑1 (MQ=255)
              gATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATtcc                                                                                                                           <  1:1173779/140‑1 (MQ=255)
                ttGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATtcctc                                                                                                                         >  2:511632/1‑140 (MQ=255)
                 tGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATtcctcc                                                                                                                        >  1:1193145/1‑140 (MQ=255)
                     gATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGttt                                                                                                                    <  1:892633/140‑1 (MQ=255)
                        aaCGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTatg                                                                                                                 <  2:406191/140‑1 (MQ=255)
                        aaCGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTatg                                                                                                                 <  2:214065/140‑1 (MQ=255)
                        aaCGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTatg                                                                                                                 <  2:246878/140‑1 (MQ=255)
                          cGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTatgat                                                                                                               >  2:1327782/1‑140 (MQ=255)
                          cGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTatgat                                                                                                               <  2:1193145/140‑1 (MQ=255)
                                   gTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGGCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGa                                                                                                      <  2:295970/140‑1 (MQ=255)
                                         aTTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCt                                                                                                >  1:757064/1‑140 (MQ=255)
                                             cAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAg                                                                                            >  2:721989/1‑140 (MQ=255)
                                             cAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAg                                                                                            >  1:120557/1‑140 (MQ=255)
                                               ggAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGa                                                                                          >  1:474008/1‑140 (MQ=255)
                                               ggAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGa                                                                                          >  1:304469/1‑140 (MQ=255)
                                                    tGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCgg                                                                                     <  1:511632/140‑1 (MQ=255)
                                                       cgcATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCggtgg                                                                                  >  2:714114/1‑140 (MQ=255)
                                                            gggCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAAt                                                                                        <  2:496444/129‑1 (MQ=255)
                                                            gggCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAAt                                                                                        >  1:496444/1‑129 (MQ=255)
                                                             ggCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCAtt                                                                            <  1:903949/140‑1 (MQ=255)
                                                                 tATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACt                                                                        >  1:37598/1‑140 (MQ=255)
                                                                 tATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACt                                                                        <  1:1080231/140‑1 (MQ=255)
                                                                        ccgGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAAcc                                                                 >  1:944007/1‑140 (MQ=255)
                                                                        ccgGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAAcc                                                                 >  1:419313/1‑140 (MQ=255)
                                                                        ccgGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGCAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAAcc                                                                 >  2:136724/1‑140 (MQ=255)
                                                                             aTTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCaa                                                            <  1:257133/140‑1 (MQ=255)
                                                                                    ccGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGc                                                     <  1:721989/140‑1 (MQ=255)
                                                                                            aaGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAg                                             <  2:503306/140‑1 (MQ=255)
                                                                                                  cGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTc                                       >  2:953280/1‑140 (MQ=255)
                                                                                                  cGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTc                                       >  1:61600/1‑140 (MQ=255)
                                                                                                    tGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCa                                     >  1:990838/1‑140 (MQ=255)
                                                                                                      ggCAATATCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGc                                   >  2:359904/1‑140 (MQ=255)
                                                                                                           tatCAATAATGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGAc                              <  2:944007/140‑1 (MQ=255)
                                                                                                                ataatGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAAtct                         >  2:1322552/1‑140 (MQ=255)
                                                                                                                 taatGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAAtctc                        <  2:243526/140‑1 (MQ=255)
                                                                                                                    tGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGc                     >  1:1348503/1‑140 (MQ=255)
                                                                                                                    tGCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGc                     >  1:318346/1‑140 (MQ=255)
                                                                                                                     gCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGcc                    >  1:667899/1‑140 (MQ=255)
                                                                                                                     gCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGcc                    >  1:298974/1‑140 (MQ=255)
                                                                                                                     gCGGTAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCAGcc                    >  1:933766/1‑140 (MQ=255)
                                                                                                                         tAATGGCGCGTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGAtt                >  1:770879/1‑140 (MQ=255)
                                                                                                                                 cgTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTgcgc        <  1:182229/140‑1 (MQ=255)
                                                                                                                                  gTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCa       <  2:770879/140‑1 (MQ=255)
                                                                                                                                  gTCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCa       <  2:1215759/140‑1 (MQ=255)
                                                                                                                                   tCTGGGGG‑AAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCaa      >  1:278590/1‑140 (MQ=255)
                                                                                                                                        ggggAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGAtt  <  2:318346/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
CCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATT  >  NC_000913/3065483‑3065758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A