Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,088,440	A→G	T53A (ACG→GCG)	galP →	galactose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,088,440	0	A	G	100.0%	184.3 / NA	51	T53A (ACG→GCG)	galP	galactose:H(+) symporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/30); total (21/30)

AGGGGCGGTCAAACAAGGCAATGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACACGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTT  >  NC_000913/3088303‑3088576

aGGGGCGGTCAAACAAGGCAATGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCAcgcg                                                                                                                                        >  2:586874/1‑140 (MQ=255)

    gCGGTCAAACAAGGCAATGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAg                                                                                                                                    <  1:583852/140‑1 (MQ=255)

       gTCAAACAAGGCAATGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAAt                                                                                                                                 >  1:487464/1‑140 (MQ=255)

       gTCAAACAAGGCAATGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAAt                                                                                                                                 >  1:1323242/1‑140 (MQ=255)

       gTCAAACAAGGCAATGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAAt                                                                                                                                 <  1:573502/140‑1 (MQ=255)

       gTCAAACAAGGCAATGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAAt                                                                                                                                 >  1:179952/1‑140 (MQ=255)

             cAAGGCAATGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCg                                                                                                                           <  1:60781/140‑1 (MQ=255)

              aaGGCAATGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGt                                                                                                                          <  1:149638/140‑1 (MQ=255)

              aaGGCAATGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGt                                                                                                                          <  2:1169660/140‑1 (MQ=255)

                 gCAATGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAg                                                                                                                       <  2:156935/140‑1 (MQ=255)

                   aaTGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCt                                                                                                                     <  1:909509/140‑1 (MQ=255)

                   aaTGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCt                                                                                                                     <  1:244346/140‑1 (MQ=255)

                   aaTGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCt                                                                                                                     <  2:1326619/140‑1 (MQ=255)

                   aaTGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCt                                                                                                                     <  1:178898/140‑1 (MQ=255)

                   aaTGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCt                                                                                                                     <  1:1345582/140‑1 (MQ=255)

                   aaTGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCt                                                                                                                     <  1:1290127/140‑1 (MQ=255)

                      gACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCa                                                                                                                  >  2:233524/1‑140 (MQ=255)

                      gACGTTTTTCGTCTGCTTCCTTACCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCa                                                                                                                  >  1:734199/1‑140 (MQ=255)

                       aCGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCat                                                                                                                 <  1:454138/140‑1 (MQ=255)

                       aCGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCat                                                                                                                 <  2:1358228/140‑1 (MQ=255)

                        cGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCatg                                                                                                                >  1:1372062/1‑140 (MQ=255)

                           ttttCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCatgatg                                                                                                             <  1:1311517/140‑1 (MQ=255)

                             ttCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGtt                                                                                                           <  2:986878/140‑1 (MQ=255)

                             ttCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGtt                                                                                                           <  2:266276/140‑1 (MQ=255)

                             ttCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGtt                                                                                                           <  2:725558/140‑1 (MQ=255)

                                       tCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCa                                                                                                 <  1:359315/140‑1 (MQ=255)

                                                ctctGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTcggtgcg                                                                                        <  2:163761/140‑1 (MQ=255)

                                                  ctGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTcggtgcggt                                                                                      <  2:179952/140‑1 (MQ=255)

                                                  ctGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTcggtgcggt                                                                                      >  1:1200027/1‑140 (MQ=255)

                                                                 tttGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCggctggct                                                                       <  2:296751/140‑1 (MQ=255)

                                                                  ttGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGctc                                                                      <  2:487464/140‑1 (MQ=255)

                                                                   tGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGctct                                                                     >  1:78446/1‑140 (MQ=255)

                                                                   tGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGctct                                                                     >  1:851867/1‑140 (MQ=255)

                                                                         ggATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTa                                                               >  1:1001108/1‑140 (MQ=255)

                                                                          gATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTaa                                                              >  1:876342/1‑140 (MQ=255)

                                                                           atatCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTaaa                                                             >  1:642986/1‑140 (MQ=255)

                                                                             atCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACt                                                           >  1:194801/1‑140 (MQ=255)

                                                                               cGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCg                                                         <  2:1331909/140‑1 (MQ=255)

                                                                                      aTTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCaa                                                  >  2:1016945/1‑140 (MQ=255)

                                                                                       ttGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCaaa                                                 <  2:1372062/140‑1 (MQ=255)

                                                                                       ttGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCaaa                                                 <  2:734199/140‑1 (MQ=255)

                                                                                         gCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCaaaaa                                               <  2:191765/140‑1 (MQ=255)

                                                                                                               cAGATGAATTCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAAtt                         >  1:674868/1‑140 (MQ=255)

                                                                                                                       ttCCAGATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTttgtttgt                 >  1:571809/1‑140 (MQ=255)

                                                                                                                            gATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTTTGTTGCCg            >  2:271290/1‑140 (MQ=255)

                                                                                                                            gATTACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTTTGTTGCCg            <  2:1323242/140‑1 (MQ=255)

                                                                                                                               tACTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGtt         >  1:493882/1‑140 (MQ=255)

                                                                                                                                aCTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTttgttg               <  2:1197610/133‑1 (MQ=255)

                                                                                                                                aCTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTttgttg               >  1:1197610/1‑133 (MQ=255)

                                                                                                                                 cTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCg       >  2:353930/1‑140 (MQ=255)

                                                                                                                                 cTTCGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCg       >  2:816294/1‑140 (MQ=255)

                                                                                                                                    cGCACGCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTg    <  2:78446/140‑1 (MQ=255)

                                                                                                                                      cacgCGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGtt  >  2:281081/1‑140 (MQ=255)

AGGGGCGGTCAAACAAGGCAATGACGTTTTTCGTCTGCTTCCTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTGCCGTTTATTGCAGATGAATTCCAGATTACTTCGCACACGCAAGAATGGGTCGTAAGCTCCATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTT  >  NC_000913/3088303‑3088576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A