Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,224,233	A→G	H534R (CAC→CGC)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,224,233	0	A	G	100.0%	404.7 / NA	110	H534R (CAC→CGC)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (48/62); total (48/62)

Only 100 of 112 total aligned reads displayed.

ATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGA  >  NC_000913/3224097‑3224368

aTGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGa                                                                                                                                      <  2:1333930/140‑1 (MQ=255)

aTGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGa                                                                                                                                      <  1:655552/140‑1 (MQ=255)

aTGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGa                                                                                                                                      >  1:1318528/1‑140 (MQ=255)

 tGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGAt                                                                                                                                     >  2:363455/1‑140 (MQ=255)

 tGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGAt                                                                                                                                     >  2:1038940/1‑140 (MQ=255)

   tACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTg                                                                                                                                   >  1:269347/1‑140 (MQ=255)

       cccGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCAtt                                                                                                                               <  2:939977/140‑1 (MQ=255)

       cccGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCAtt                                                                                                                               <  2:846123/140‑1 (MQ=255)

       cccGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCAtt                                                                                                                               <  2:510040/140‑1 (MQ=255)

       cccGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCAtt                                                                                                                               <  1:270523/140‑1 (MQ=255)

       cccGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTAATGGGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCAtt                                                                                                                               <  2:703960/140‑1 (MQ=255)

        ccGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTc                                                                                                                              >  1:1268980/1‑140 (MQ=255)

        ccGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTc                                                                                                                              >  2:459038/1‑140 (MQ=255)

         cgcgTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCa                                                                                                                             <  1:844090/140‑1 (MQ=255)

         cgcgTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCa                                                                                                                             >  1:1105348/1‑140 (MQ=255)

         cgcgTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCa                                                                                                                             <  2:814680/140‑1 (MQ=255)

         cgcgTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCa                                                                                                                             <  2:197436/140‑1 (MQ=255)

         cgcgTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGAGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCa                                                                                                                             <  2:482377/140‑1 (MQ=255)

           cgTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAgg                                                                                                                           >  2:195073/1‑140 (MQ=255)

           cgTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAgg                                                                                                                           >  1:458141/1‑140 (MQ=255)

           cgTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAgg                                                                                                                           >  2:71335/1‑140 (MQ=255)

           cgTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAgg                                                                                                                           <  2:458141/140‑1 (MQ=255)

           cgTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAgg                                                                                                                           >  1:1368584/1‑140 (MQ=255)

            gTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAggg                                                                                                                          >  2:778181/1‑140 (MQ=255)

            gTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAggg                                                                                                                          >  1:30909/1‑140 (MQ=255)

             tcccGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAgggc                                                                                                                         >  1:851744/3‑139 (MQ=255)

             tGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCAtt                                                                                                                               >  2:1143060/1‑134 (MQ=255)

             tGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCAtt                                                                                                                               <  1:1143060/134‑1 (MQ=255)

               ccGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCa                                                                                                                       <  1:1154054/140‑1 (MQ=255)

                cGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCAt                                                                                                                      <  1:1360323/140‑1 (MQ=255)

                 gCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCAtt                                                                                                                     <  2:269347/140‑1 (MQ=255)

                 gCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCAATTGCATTCAGGGTCAtt                                                                                                                     <  2:821845/140‑1 (MQ=255)

                 gCTGATGAATCAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCAtt                                                                                                                     <  1:1316235/140‑1 (MQ=255)

                  cTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTGATTa                                                                                                                    >  2:1300167/1‑140 (MQ=255)

                  cTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCAtga                                                                                                                    >  2:1308379/1‑138 (MQ=255)

                  cTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTa                                                                                                                    >  2:1071360/1‑140 (MQ=255)

                    gatgaATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAgg                                                                                                                           <  2:158445/131‑1 (MQ=255)

                    gatgaATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAgg                                                                                                                           >  1:158445/1‑131 (MQ=255)

                          tgaGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGTGTCATTATGTCTggg                                                                                                            >  1:497418/1‑140 (MQ=255)

                          tgaGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTggg                                                                                                            >  1:668108/1‑140 (MQ=255)

                            aGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGa                                                                                                           >  1:1413967/1‑139 (MQ=255)

                            aGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGa                                                                                                           <  2:1413967/139‑1 (MQ=255)

                            aGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAg                                                                                                          <  1:1020704/140‑1 (MQ=255)

                               ttGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAgtgg                                                                                                       >  1:934288/1‑140 (MQ=255)

                               ttGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAgtgg                                                                                                       >  2:936996/1‑140 (MQ=255)

                                  gTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGc                                                                                                    <  2:1066725/140‑1 (MQ=255)

                                     aaTACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGAc                                                                                                 <  1:778181/140‑1 (MQ=255)

                                        aCCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCAc                                                                                              <  1:1308379/140‑1 (MQ=255)

                                        aCCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCAc                                                                                              <  1:1071360/140‑1 (MQ=255)

                                             cATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGAt                                                                                         <  1:195073/140‑1 (MQ=255)

                                             cATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGAt                                                                                         >  1:111907/1‑140 (MQ=255)

                                              aTCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATc                                                                                        <  1:132723/140‑1 (MQ=255)

                                              aTCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATc                                                                                        <  1:1376203/140‑1 (MQ=255)

                                              aTCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATc                                                                                        <  1:250330/140‑1 (MQ=255)

                                               tCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATcc                                                                                       >  1:402757/1‑140 (MQ=255)

                                                  gAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAgg                                                                                    <  1:1038940/140‑1 (MQ=255)

                                                  gAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAgg                                                                                    <  1:363455/140‑1 (MQ=255)

                                                  gAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAgg                                                                                    <  2:1268980/140‑1 (MQ=255)

                                                   aaGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGc                                                                                   <  1:611044/140‑1 (MQ=255)

                                                    aGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGca                                                                                  >  1:1122455/1‑140 (MQ=255)

                                                        gcgcATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAgg                                                                              <  2:1318528/140‑1 (MQ=255)

                                                        gcgcATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAgg                                                                              <  1:1014478/140‑1 (MQ=255)

                                                        gcgcATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAgg                                                                              >  2:1188636/1‑140 (MQ=255)

                                                                tcTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGcaca                                                                                >  2:124839/1‑130 (MQ=255)

                                                                tcTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGcaca                                                                                <  1:124839/130‑1 (MQ=255)

                                                                 cTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACg                                                                     <  2:1105348/140‑1 (MQ=255)

                                                                        tatGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGt                                                              <  2:668108/140‑1 (MQ=255)

                                                                        tatGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGt                                                              <  1:838985/140‑1 (MQ=255)

                                                                           gCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTg                                                           >  1:891261/1‑140 (MQ=255)

                                                                                tGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGtata                                                      >  2:368553/1‑140 (MQ=255)

                                                                                tGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGtata                                                      >  1:573778/1‑140 (MQ=255)

                                                                                   gATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAAt                                                   >  2:1090119/1‑140 (MQ=255)

                                                                                           aCGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCggcggc                                           >  1:92416/1‑140 (MQ=255)

                                                                                             ggACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGcggcga                                         >  1:625005/1‑140 (MQ=255)

                                                                                               aCCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGcggcgact                                       >  2:1064375/1‑140 (MQ=255)

                                                                                               aCCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCAGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCAGGTATAAATTCGGcggcgact                                       >  2:1284963/1‑140 (MQ=255)

                                                                                                 cgggcgggCTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGcggcgactac                                     <  2:1368584/140‑1 (MQ=255)

                                                                                                  gggcgggcTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGcggcgactacg                                    <  2:625005/140‑1 (MQ=255)

                                                                                                    gcgggcTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGcggcgactacggc                                  >  2:46200/1‑140 (MQ=255)

                                                                                                     cgggcTGACGGAGTACCAGAATGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGcggcgactacggcg                                 <  2:92416/140‑1 (MQ=255)

                                                                                                      gggcTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGcggcgactacggcga                                <  1:936996/140‑1 (MQ=255)

                                                                                                      gggcTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGcggcgactacggcga                                <  2:934288/140‑1 (MQ=255)

                                                                                                       ggcTGACGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGcggcgactacggcgac                               <  2:350791/140‑1 (MQ=255)

                                                                                                             cGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGATCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATccc                         <  2:1122455/140‑1 (MQ=255)

                                                                                                             cGGAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATccc                         <  2:851744/140‑1 (MQ=255)

                                                                                                              ggAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCca                        <  1:1007194/140‑1 (MQ=255)

                                                                                                               gAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCcaa                       <  2:573778/140‑1 (MQ=255)

                                                                                                               gAGTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCcaa                       <  2:402757/140‑1 (MQ=255)

                                                                                                                 gTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCcaaca                     <  1:348778/140‑1 (MQ=255)

                                                                                                                 gTACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCcaaca                     <  1:1241768/140‑1 (MQ=255)

                                                                                                                  tACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCcaacaa                    >  1:659660/1‑140 (MQ=255)

                                                                                                                  tACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCcaa                       >  2:159193/1‑137 (MQ=255)

                                                                                                                  tACCAGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCcaa                       <  1:159193/137‑1 (MQ=255)

                                                                                                                      aGAACGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACtat                <  2:111907/140‑1 (MQ=255)

                                                                                                                          cGTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACt            >  1:689218/1‑140 (MQ=255)

                                                                                                                           gTCTTCTATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACtt           <  2:668258/140‑1 (MQ=255)

                                                                                                                            tcttctATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTc          >  1:265792/1‑140 (MQ=255)

                                                                                                                              ttctATAAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTg        >  2:227827/1‑140 (MQ=255)

                                                                                                                                   taAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTg   <  2:371906/140‑1 (MQ=255)

                                                                                                                                   taAGCGCGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTg   <  1:1090119/140‑1 (MQ=255)

ATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGA  >  NC_000913/3224097‑3224368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A