Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 191 83.5 2830284 99.3% 2810472 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,231,325 T→C intergenic (+86/‑340) ygjK → / → fadH glycoside hydrolase/2,4‑dienoyl‑CoA reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,231,3250TC100.0% 171.7 / NA 48intergenic (+86/‑340)ygjK/fadHglycoside hydrolase/2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/22);  total (26/22)

GCCGCGCATTTGTATATGTTGTATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGT  >  NC_000913/3231192‑3231393
                                                                                                                                     |                                                                    
gCCGCGCATTTGTATATGTTGTATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATcc                                                                <  2:883485/140‑1 (MQ=255)
gCCGCGCATTTGTATATGTTGTATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATcc                                                                <  1:90392/140‑1 (MQ=255)
 ccGCGCATTTGTATATGTTGTATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCg                                                               >  2:420309/1‑140 (MQ=255)
      cATTTGTATATGTTGTATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCatc                                                          >  1:385367/1‑138 (MQ=255)
             atatGTTGTATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACcg                                                   <  1:628340/140‑1 (MQ=255)
                 gttgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCTCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATcc                                                                >  2:204983/1‑123 (MQ=255)
                 gttgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCTCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATcc                                                                <  1:204983/123‑1 (MQ=255)
                 gttgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGcc                                               <  1:655468/140‑1 (MQ=255)
                 gttgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGcc                                               >  2:780458/1‑140 (MQ=255)
                 gttgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGCGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGcc                                               >  2:711036/1‑140 (MQ=255)
                  ttgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCa                                              <  1:780458/140‑1 (MQ=255)
                   tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  2:757812/1‑140 (MQ=255)
                   tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  1:413143/1‑140 (MQ=255)
                   tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  1:415681/1‑140 (MQ=255)
                   tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  2:817715/1‑140 (MQ=255)
                   tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  2:1055874/1‑140 (MQ=255)
                   tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  1:906699/1‑140 (MQ=255)
                   tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  2:641231/1‑140 (MQ=255)
                   tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  1:832234/1‑140 (MQ=255)
                   tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  1:900893/1‑140 (MQ=255)
                   tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGCCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  2:924039/1‑140 (MQ=255)
                     tataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTg                                           >  2:1040404/1‑140 (MQ=255)
                     tataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTg                                           >  2:154180/1‑140 (MQ=255)
                      ataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACcgcg                                                 <  2:552275/133‑1 (MQ=255)
                      ataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACcgcg                                                 >  1:552275/1‑133 (MQ=255)
                      ataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTgg                                          >  1:894578/1‑140 (MQ=255)
                      ataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTgg                                          >  2:544678/1‑140 (MQ=255)
                          cGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCAcc                                                    <  1:171248/126‑1 (MQ=255)
                          cGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCAcc                                                    >  2:171248/1‑126 (MQ=255)
                          cGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACcg                                                   <  2:283677/127‑1 (MQ=255)
                          cGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACcg                                                   >  1:283677/1‑127 (MQ=255)
                              tttttCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATgcg                                  <  1:154180/140‑1 (MQ=255)
                               ttttCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACTCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATgccg                                 >  1:104884/1‑138 (MQ=255)
                               ttttCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATgcgg                                 >  2:354039/1‑140 (MQ=255)
                                   ccGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTg                             <  2:1410442/140‑1 (MQ=255)
                                   ccGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTg                             <  2:344532/140‑1 (MQ=255)
                                   ccGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTg                             <  1:357463/140‑1 (MQ=255)
                                      tAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGAc                          >  2:773268/1‑140 (MQ=255)
                                        aGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGc                        <  1:1055874/140‑1 (MQ=255)
                                             taatTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTAt                   <  1:366957/140‑1 (MQ=255)
                                              aatTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATc                  <  1:757812/140‑1 (MQ=255)
                                                 ttGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGc               <  2:1224726/140‑1 (MQ=255)
                                                 ttGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGc               <  1:641231/140‑1 (MQ=255)
                                                           tgGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACAtgtg     <  1:924039/140‑1 (MQ=255)
                                                            ggCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACAtgtgc    >  2:1274568/1‑139 (MQ=255)
                                                             gCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACAtgtgca   <  2:385367/140‑3 (MQ=255)
                                                             gCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACAtgtgca   <  1:1144699/140‑3 (MQ=255)
                                                              cGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACAtgtgcaa  <  1:1040404/140‑4 (MQ=255)
                                                                                                                                     |                                                                    
GCCGCGCATTTGTATATGTTGTATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGT  >  NC_000913/3231192‑3231393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A