Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,294,582	T→C	L395L (TTG→CTG)	lpoA →	outer membrane lipoprotein ‑ activator of MrcA activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,294,582	0	T	C	100.0%	288.5 / NA	78	L395L (TTG→CTG)	lpoA	outer membrane lipoprotein ‑ activator of MrcA activity
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (34/44); total (34/44)

CGCACCCGCAAATCCATCCGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGCGCGCGATGCAGCGCGTCATATTCGTGACCA  >  NC_000913/3294446‑3294718

cgcACCCGCAAATCCATCCGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGc                                                                                                                                       <  1:674473/140‑1 (MQ=255)

cgcACCCGCAAATCCATCCGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGc                                                                                                                                       <  2:185690/140‑1 (MQ=255)

cgcACCCGCAAATCCATCCGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGc                                                                                                                                       <  1:1210825/140‑1 (MQ=255)

  cACCCGCAAATCCATCCGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGctgctg                                                                                                                                     <  1:1086523/140‑1 (MQ=255)

   aCCCGCAAATCCATCCGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGa                                                                                                                                    >  2:506887/1‑140 (MQ=255)

   aCCCGCAAATCCATCCGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGa                                                                                                                                    >  2:1105602/1‑140 (MQ=255)

     ccGCAAATCCATCCGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAag                                                                                                                                  >  1:1291554/1‑140 (MQ=255)

       gCAAATCCATCCGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAagag                                                                                                                                <  2:979466/140‑1 (MQ=255)

        cAAATCCATCCGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGc                                                                                                                               >  2:231014/1‑140 (MQ=255)

           atccatccGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAac                                                                                                                            <  1:231014/140‑1 (MQ=255)

            tccatccGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAaca                                                                                                                           <  2:989884/140‑1 (MQ=255)

            tccatccGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAaca                                                                                                                           <  2:1281834/140‑1 (MQ=255)

             ccatccGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTAGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAacac                                                                                                                          <  1:979798/140‑1 (MQ=255)

              catccGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACt                                                                                                                         <  2:1291554/140‑1 (MQ=255)

               atccGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTc                                                                                                                        >  1:510435/1‑140 (MQ=255)

               atccGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTc                                                                                                                        >  1:1211340/1‑140 (MQ=255)

                   gCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCt                                                                                                                    <  2:729225/140‑1 (MQ=255)

                      gagCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGaa                                                                                                                 <  2:449449/140‑1 (MQ=255)

                          tGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTa                                                                                                             <  2:834714/140‑1 (MQ=255)

                                tCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCa                                                                                                       <  2:510435/140‑1 (MQ=255)

                                tCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCa                                                                                                       <  2:1211340/140‑1 (MQ=255)

                                tCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCa                                                                                                       <  2:73085/140‑1 (MQ=255)

                                tCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCa                                                                                                       <  1:1367275/140‑1 (MQ=255)

                                          cTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc                                                                                             >  2:1109718/1‑140 (MQ=255)

                                          cTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc                                                                                             >  2:914398/1‑140 (MQ=255)

                                          cTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc                                                                                             >  2:523207/1‑140 (MQ=255)

                                          cTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc                                                                                             >  1:341476/1‑140 (MQ=255)

                                               cacaACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAAc                                                                                                 >  2:914154/1‑131 (MQ=255)

                                               cacaACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAAc                                                                                                 <  1:914154/131‑1 (MQ=255)

                                                acaACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGgaga                                                                                       <  1:1105602/140‑1 (MQ=255)

                                                acaACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGgaga                                                                                       <  1:516910/140‑1 (MQ=255)

                                                    ccACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAatat                                                                                   <  1:885416/140‑1 (MQ=255)

                                                         tAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGaaa                                                                              >  2:492751/1‑140 (MQ=255)

                                                          aGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGaaaa                                                                             >  2:71832/1‑140 (MQ=255)

                                                               gATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATcgcg                                                                        <  1:1323475/140‑1 (MQ=255)

                                                                aTCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGt                                                                       <  1:1130358/140‑1 (MQ=255)

                                                                aTCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGt                                                                       >  1:185668/1‑140 (MQ=255)

                                                                  cTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCa                                                                     <  2:716467/140‑1 (MQ=255)

                                                                     aaGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGt                                                                       <  1:856909/135‑1 (MQ=255)

                                                                     aaGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGt                                                                       >  2:856909/1‑135 (MQ=255)

                                                                          aGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGt                                                             >  1:305376/1‑140 (MQ=255)

                                                                          aGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGt                                                             >  1:624913/1‑140 (MQ=255)

                                                                           ggTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGtt                                                            >  2:187826/1‑140 (MQ=255)

                                                                            gTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTa                                                           >  2:846034/1‑140 (MQ=255)

                                                                            gTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTa                                                           >  1:426712/1‑140 (MQ=255)

                                                                            gTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTa                                                           >  2:251337/1‑140 (MQ=255)

                                                                            gTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTa                                                           >  1:1194650/1‑140 (MQ=255)

                                                                                 gcagGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTcg                                                      >  2:424982/1‑140 (MQ=255)

                                                                                   agGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTcgcg                                                    <  2:1194650/140‑1 (MQ=255)

                                                                                    ggATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCgcgc                                                   >  1:681394/1‑140 (MQ=255)

                                                                                       tGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCttt                                                <  1:251337/140‑1 (MQ=255)

                                                                                       tGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCttt                                                >  2:258224/1‑140 (MQ=255)

                                                                                          cgcgAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCAc                                             >  1:555727/1‑140 (MQ=255)

                                                                                          cgcgAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCAc                                             >  2:1142066/1‑140 (MQ=255)

                                                                                           gcgAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCAcc                                            <  1:187826/140‑1 (MQ=255)

                                                                                           gcgAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCAcc                                            <  2:305376/140‑1 (MQ=255)

                                                                                                tATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAg                                       >  1:622068/1‑140 (MQ=255)

                                                                                                     tgGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGaa                                  <  2:681394/140‑1 (MQ=255)

                                                                                                       gtcggtcCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAgc                                <  1:1205607/140‑1 (MQ=255)

                                                                                                        tcggtcCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAgcg                               <  1:579217/140‑1 (MQ=255)

                                                                                                        tcggtcCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAgcg                               >  2:723975/1‑140 (MQ=255)

                                                                                                        tcggtcCGTTGCTGAAAAAT‑ACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACGGGAAGACGAAgcgc                              >  1:31669/1‑140 (MQ=255)

                                                                                                          ggtcCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAgcgcg                             >  2:80397/1‑140 (MQ=255)

                                                                                                            tcCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGcgcgcg                           >  1:1391148/1‑140 (MQ=255)

                                                                                                            tcCGTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGcgcgcg                           <  1:914398/140‑1 (MQ=255)

                                                                                                               gTTGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGCGCGCGATg                        >  2:1378862/1‑140 (MQ=255)

                                                                                                                 tGCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGCGCGCGATGCa                      <  1:723975/140‑1 (MQ=255)

                                                                                                                  gCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGCGCGCGATGCAg                     >  1:698060/1‑140 (MQ=255)

                                                                                                                  gCTGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGCGCGCGATGCAg                     <  2:624913/140‑1 (MQ=255)

                                                                                                                    tGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGCGCGCGATGCAgcg                   <  1:846034/140‑1 (MQ=255)

                                                                                                                    tGAAAAATAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGCGCGCGATGCAgcg                   <  1:80397/140‑1 (MQ=255)

                                                                                                                       aaaaTAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGCGCGCGATGcgcttcgt                <  2:341476/140‑8 (MQ=255)

                                                                                                                         aaTAACGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGCGCGCGATGCAGCGCGTCa              <  1:71832/140‑1 (MQ=255)

                                                                                                                             aCGTTGAAGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGCGCGCGATGCAGCGCGTCATAtt          >  2:678419/1‑140 (MQ=255)

                                                                                                                                   aaGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGCGCGCGATGCAGCGCGTCATATTCGTGAc    <  1:1381693/140‑1 (MQ=255)

                                                                                                                                   aaGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGCGCGCGATGCAGCGCGTCATATTCGTGAc    <  2:698060/140‑1 (MQ=255)

                                                                                                                                   aaGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGCGCGCGATGCAGCGCGTCATATTCGTGAc    <  2:622068/140‑1 (MQ=255)

                                                                                                                                   aaGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGCGCGCGATGCAGCGCGTCATATTCGTGAc    <  2:426712/140‑1 (MQ=255)

                                                                                                                                   aaGAGCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGCGCGCGATGCAGCGCGTCATATTCGTGAc    <  1:678419/140‑1 (MQ=255)

                                                                                                                                     gagCTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGCGCGCGATGCAGCGCGTCATATTCGTGACCa  <  2:421258/140‑1 (MQ=255)

CGCACCCGCAAATCCATCCGCAGAGCTGAAAATCTACGATACCTCATCACAACCACTTAGCCAGATCTTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGTCAATATTTGTTACTTCGCGCTTTCACCGGAAGACGAAGCGCGCGATGCAGCGCGTCATATTCGTGACCA  >  NC_000913/3294446‑3294718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A