Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,319,286	T→C	V217A (GTC→GCC)	argG →	argininosuccinate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,319,286	0	T	C	98.9%	318.2 / ‑4.5	88	V217A (GTC→GCC)	argG	argininosuccinate synthetase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (51/36); total (52/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

ATGTCTGAATTTATGATTGCCTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCT  >  NC_000913/3319150‑3319421

aTGTCTGAATTTATGATTGCCTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCaa                                                                                                                                      <  2:510962/140‑1 (MQ=255)

   tCTGAATTTATGATTGCCTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAATGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAAccc                                                                                                                                   >  2:489063/1‑140 (MQ=255)

   tCTGAATTTATGATTGCCTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAGGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAAccc                                                                                                                                   >  2:1303350/1‑140 (MQ=255)

   tCTGAATTTATGATTGCCTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAAccc                                                                                                                                   >  1:1126218/1‑140 (MQ=255)

   tCTGAATTTATGATTGCCTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAAccc                                                                                                                                   >  2:1114419/1‑140 (MQ=255)

         tttATGATTGCCTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTAt                                                                                                                             <  1:1161237/140‑1 (MQ=255)

         tttATGATTGCCTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTAt                                                                                                                             <  2:201460/140‑1 (MQ=255)

           tATGATTGCCTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATgg                                                                                                                           <  2:699429/140‑1 (MQ=255)

            aTGATTGCCTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATggg                                                                                                                          >  2:488491/1‑140 (MQ=255)

                ttGCCTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAATGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTg                                                                                                                      <  1:489063/140‑1 (MQ=255)

                ttGCCTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGCTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTg                                                                                                                      <  2:873574/140‑1 (MQ=255)

                  gCCTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGaa                                                                                                                    <  1:1193732/140‑1 (MQ=255)

                   ccTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGaaa                                                                                                                   >  1:1172499/1‑140 (MQ=255)

                   ccTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGaaa                                                                                                                   >  2:622932/1‑140 (MQ=255)

                    cTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAAt                                                                                                                  <  2:1176538/140‑1 (MQ=255)

                    cTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAAt                                                                                                                  <  1:741725/140‑1 (MQ=255)

                    cTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAAt                                                                                                                  <  2:472026/140‑1 (MQ=255)

                    cTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAAt                                                                                                                  <  1:622932/140‑1 (MQ=255)

                     tGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAAtt                                                                                                                 >  1:917941/1‑140 (MQ=255)

                     tGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAAtt                                                                                                                 >  1:172489/1‑140 (MQ=255)

                       cGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCt                                                                                                               <  1:582267/140‑1 (MQ=255)

                             cGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATg                                                                                                         <  1:693959/140‑1 (MQ=255)

                               aCTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTAAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATgag                                                                                                       >  1:202014/1‑140 (MQ=255)

                                cTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTAAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATgaga                                                                                                      <  2:202014/140‑1 (MQ=255)

                                          tctgtcGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGAt                                                                                            <  1:1250748/140‑1 (MQ=255)

                                             gtcGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATccc                                                                                         >  1:756038/1‑140 (MQ=255)

                                             gtcGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATccc                                                                                         >  1:855476/1‑140 (MQ=255)

                                             gtcGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATccc                                                                                         >  2:483177/1‑140 (MQ=255)

                                                        cTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGaagaag                                                                              <  2:967776/140‑1 (MQ=255)

                                                        cTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGaagaag                                                                              <  2:1373286/140‑1 (MQ=255)

                                                           cTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTca                                                                           >  1:444890/1‑140 (MQ=255)

                                                           cTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTca                                                                           >  1:1175379/1‑140 (MQ=255)

                                                               acaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGt                                                                       <  1:197142/140‑1 (MQ=255)

                                                                 aGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTAc                                                                     >  1:768930/1‑140 (MQ=255)

                                                                   aCTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGc                                                                   >  1:853391/1‑140 (MQ=255)

                                                                    cTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCt                                                                  <  2:500837/140‑1 (MQ=255)

                                                                     tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCtt                                                                 >  2:1354825/1‑140 (MQ=255)

                                                                     tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCtt                                                                 >  1:1191055/1‑140 (MQ=255)

                                                                     tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCtt                                                                 >  1:478505/1‑140 (MQ=255)

                                                                      ccAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCttt                                                                >  1:888120/1‑140 (MQ=255)

                                                                        aaCATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGa                                                              >  2:474427/1‑140 (MQ=255)

                                                                        aaCATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGa                                                              >  1:1406098/1‑140 (MQ=255)

                                                                          cATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAAc                                                            >  1:649800/1‑140 (MQ=255)

                                                                             gCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAg                                                         >  1:1329699/1‑140 (MQ=255)

                                                                              cTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAgg                                                        <  1:1033986/140‑1 (MQ=255)

                                                                               ttGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGt                                                       >  1:1301241/1‑140 (MQ=255)

                                                                               ttGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGt                                                       >  1:541434/1‑140 (MQ=255)

                                                                               ttGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGt                                                       >  1:1251329/1‑140 (MQ=255)

                                                                                      aaCGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCgg                                                <  2:975245/140‑1 (MQ=255)

                                                                                       aCGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGt                                               >  1:562005/1‑140 (MQ=255)

                                                                                        cGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTg                                              >  1:1366234/1‑140 (MQ=255)

                                                                                        cGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTg                                              >  2:603161/1‑140 (MQ=255)

                                                                                        cGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTg                                              >  2:1240051/1‑140 (MQ=255)

                                                                                           aTGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGgcg                                           <  2:756038/140‑1 (MQ=255)

                                                                                               aGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGa                                       <  2:768930/140‑1 (MQ=255)

                                                                                               aGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAACTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGa                                       <  2:1126218/140‑1 (MQ=255)

                                                                                                   aaGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACgg                                   >  1:500387/1‑140 (MQ=255)

                                                                                                    aGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGt                                  >  1:218853/1‑140 (MQ=255)

                                                                                                      gATCTGGAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTaa                                <  1:277192/140‑1 (MQ=255)

                                                                                                            gAATACCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCtt                          >  1:1328996/1‑140 (MQ=255)

                                                                                                                aCCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGc                      >  2:991361/1‑140 (MQ=255)

                                                                                                                aCCTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGc                      >  1:741891/1‑140 (MQ=255)

                                                                                                                 ccTCAACTCCAGCGTCAAAATCGCCAACCTGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGcg                     >  1:1397417/1‑140 (MQ=255)

                                                                                                                 ccTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGcg                     >  1:905853/1‑140 (MQ=255)

                                                                                                                 ccTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGcg                     <  2:917941/140‑1 (MQ=255)

                                                                                                                 ccTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGcg                     >  1:627059/1‑140 (MQ=255)

                                                                                                                  cTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGcga                    <  1:1354825/140‑1 (MQ=255)

                                                                                                                  cTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGcga                    >  2:1143148/1‑140 (MQ=255)

                                                                                                                  cTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGcga                    <  1:603161/140‑1 (MQ=255)

                                                                                                                  cTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGcga                    <  1:1114419/140‑1 (MQ=255)

                                                                                                                  cTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGcga                    >  1:1129059/1‑140 (MQ=255)

                                                                                                                  cTCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGcga                    >  2:532091/1‑140 (MQ=255)

                                                                                                                   tCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGcga                    <  2:154636/139‑1 (MQ=255)

                                                                                                                   tCAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGcga                    >  1:154636/1‑139 (MQ=255)

                                                                                                                    cAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGcgacg                  <  2:395315/140‑1 (MQ=255)

                                                                                                                    cAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGcgacg                  <  2:172489/140‑1 (MQ=255)

                                                                                                                    cAACTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGcgacg                  <  2:500387/140‑1 (MQ=255)

                                                                                                                      aCTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCgacgac                >  1:1145337/1‑140 (MQ=255)

                                                                                                                      aCTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCgacgac                >  1:1271160/1‑140 (MQ=255)

                                                                                                                       cTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGc                      <  2:125697/133‑1 (MQ=255)

                                                                                                                       cTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGc                      >  1:125697/1‑133 (MQ=255)

                                                                                                                       cTCCAGCGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGacgacg               >  2:103668/1‑140 (MQ=255)

                                                                                                                             cGTCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGaaa         <  2:1328996/140‑1 (MQ=255)

                                                                                                                               tCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGAAatg       >  1:1061104/1‑140 (MQ=255)

                                                                                                                               tCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGAAatg       >  1:146692/1‑140 (MQ=255)

                                                                                                                               tCAAAATCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGAAatg       >  1:504874/1‑140 (MQ=255)

                                                                                                                                  aaaTCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGAAatgatg    >  1:588641/1‑140 (MQ=255)

                                                                                                                                    aTCGCCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCt  <  2:1366234/140‑1 (MQ=255)

ATGTCTGAATTTATGATTGCCTGCGGTTTCGACTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCT  >  NC_000913/3319150‑3319421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A