Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,529,022	G→A	A118A (GCG→GCA)	yrfG →	purine nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,529,022	0	G	A	99.2%	433.6 / NA	122	A118A (GCG→GCA)	yrfG	purine nucleotidase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (55/66); total (55/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

TGGAGTGAGCAACTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCGCATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTA  >  NC_000913/3528885‑3529152

tGGAGTGAGCAACTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGcaca                                                                                                                                  >  1:1111574/1‑140 (MQ=255)

tGGAGTGAGCAACTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGcaca                                                                                                                                  >  2:1196468/1‑140 (MQ=255)

tGGAGTGAGCAACTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGcaca                                                                                                                                  >  1:1209860/1‑140 (MQ=255)

tGGAGTGAGCAACTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGcaca                                                                                                                                  >  1:749328/1‑140 (MQ=255)

tGGAGTGAGCAACTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGcaca                                                                                                                                  >  2:532623/1‑140 (MQ=255)

     tGAGCAACTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGc                                                                                                                             >  2:3668/1‑140 (MQ=255)

     tGAGCAACTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGc                                                                                                                             >  2:738544/1‑140 (MQ=255)

       aGCAACTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCg                                                                                                                              <  2:1363374/137‑1 (MQ=255)

       aGCAACTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCg                                                                                                                              >  1:1363374/1‑137 (MQ=255)

       aGCAACTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGcac                                                                                                                           >  2:1182649/1‑140 (MQ=255)

       aGCAACTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGcac                                                                                                                           >  2:1230860/1‑140 (MQ=255)

       aGCAACTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGcac                                                                                                                           >  2:49109/1‑140 (MQ=255)

       aGCAACTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGcac                                                                                                                           >  2:711326/1‑140 (MQ=255)

       aGCAACTGGGTCTGGATATCTGTGCGATGACCACCGAGACGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGcac                                                                                                                           >  2:212043/1‑140 (MQ=255)

         cAACTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACaa                                                                                                                         >  2:986927/1‑140 (MQ=255)

         cAACTGGGCCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACaa                                                                                                                         >  2:580791/1‑140 (MQ=255)

            cTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCt                                                                                                                      >  2:448116/1‑140 (MQ=255)

            cTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCt                                                                                                                      >  1:688936/1‑140 (MQ=255)

              gggTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTgg                                                                                                                    <  1:625423/140‑1 (MQ=255)

               ggTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGc                                                                                                                   >  2:868691/1‑140 (MQ=255)

                 tCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCgg                                                                                                                 >  1:201382/1‑140 (MQ=255)

                  cTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGt                                                                                                                <  2:893441/140‑1 (MQ=255)

                  cTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGt                                                                                                                <  2:465753/140‑1 (MQ=255)

                   tGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTa                                                                                                               >  2:1274936/1‑140 (MQ=255)

                   tGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTa                                                                                                               >  1:412058/1‑140 (MQ=255)

                    ggATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTaa                                                                                                              >  1:1197778/1‑140 (MQ=255)

                          cTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCATCAATGCACATCCGCACAACCTGGCGGTAAAACTTg                                                                                                        >  2:977023/1‑140 (MQ=255)

                           tgtgCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGa                                                                                                       >  1:931339/1‑140 (MQ=255)

                           tgtgCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGa                                                                                                       <  2:414948/140‑1 (MQ=255)

                             tgCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGc                                                                                                     >  1:377752/1‑140 (MQ=255)

                             tgCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGc                                                                                                     >  1:6290/1‑140 (MQ=255)

                               cGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCAt                                                                                                   >  1:50932/1‑140 (MQ=255)

                               cGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCAt                                                                                                   >  2:319840/1‑140 (MQ=255)

                                gATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATa                                                                                                  <  2:1350796/140‑1 (MQ=255)

                                 aTGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATAc                                                                                                 <  1:1274936/140‑1 (MQ=255)

                                 aTGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATAc                                                                                                 <  2:412058/140‑1 (MQ=255)

                                  tGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATAcc                                                                                                >  2:1277936/1‑140 (MQ=255)

                                     ccaccGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAg                                                                                                      >  2:145577/1‑131 (MQ=255)

                                     ccaccGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAg                                                                                                      <  1:145577/131‑1 (MQ=255)

                                       accGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCt                                                                                           >  2:371335/1‑140 (MQ=255)

                                        cccagaTGGGACCGGGTGCCGTACTGCGTGCAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTg                                                                                          <  2:866854/137‑1 (MQ=255)

                                        ccGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTg                                                                                          <  1:711326/140‑1 (MQ=255)

                                            gaTGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACg                                                                                      <  2:1197778/140‑1 (MQ=255)

                                            gaTGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACg                                                                                      >  1:299337/1‑140 (MQ=255)

                                             aTGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGc                                                                                     <  1:977023/140‑1 (MQ=255)

                                              tGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGca                                                                                    <  1:1119156/140‑1 (MQ=255)

                                              tGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGca                                                                                    <  1:209176/140‑1 (MQ=255)

                                              tGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGca                                                                                    >  1:540198/1‑140 (MQ=255)

                                              tGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGca                                                                                    <  1:1277936/140‑1 (MQ=255)

                                              tGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGca                                                                                    <  2:540198/140‑1 (MQ=255)

                                                ggACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGcaca                                                                                  >  1:1083871/1‑140 (MQ=255)

                                                ggACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGcaca                                                                                  >  1:805250/1‑140 (MQ=255)

                                                  aCCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACAcc                                                                                <  2:586916/140‑1 (MQ=255)

                                                   ccGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCt                                                                               >  2:355403/1‑140 (MQ=255)

                                                   ccGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCt                                                                               <  1:49109/140‑1 (MQ=255)

                                                   ccGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCt                                                                               <  1:212043/140‑1 (MQ=255)

                                                    cgcgTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTc                                                                                            <  2:321856/126‑1 (MQ=255)

                                                      cgTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCGCATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGa                                                                            <  1:872615/140‑1 (MQ=255)

                                                      cgTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGa                                                                            >  1:426890/1‑140 (MQ=255)

                                                      cgTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGa                                                                            >  2:954952/1‑140 (MQ=255)

                                                      cgTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGa                                                                            >  1:584251/1‑140 (MQ=255)

                                                       gTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGAt                                                                           >  2:19025/1‑140 (MQ=255)

                                                          ccGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATtta                                                                        <  1:355645/140‑1 (MQ=255)

                                                          ccGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATtta                                                                        <  2:1001014/140‑1 (MQ=255)

                                                           cGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATttat                                                                       <  2:688936/140‑1 (MQ=255)

                                                                tGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACtt                                                                  <  1:868691/140‑1 (MQ=255)

                                                                tGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACtt                                                                  <  2:1083871/140‑1 (MQ=255)

                                                                  cGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTc                                                                <  2:80105/140‑1 (MQ=255)

                                                                   gTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTcc                                                               <  1:532623/140‑1 (MQ=255)

                                                                    tGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGGTTTATTACTTTcca                                                              >  2:1174596/1‑140 (MQ=255)

                                                                      aaGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTccacc                                                            <  2:655553/140‑1 (MQ=255)

                                                                       aGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTccaccc                                                           <  2:584251/140‑1 (MQ=255)

                                                                        gATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTccaccca                                                          <  1:863259/140‑1 (MQ=255)

                                                                        gATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTccaccca                                                          <  1:337671/140‑1 (MQ=255)

                                                                            ccATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCcacaca                                                      >  1:1369927/1‑140 (MQ=255)

                                                                              aTTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACAtt                                                    >  1:1082099/1‑140 (MQ=255)

                                                                                 ccGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCTACCCACACATTTgg                                                 <  1:1196468/140‑1 (MQ=255)

                                                                                 ccGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTgg                                                 <  2:1311630/140‑1 (MQ=255)

                                                                                 ccGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTgg                                                 <  2:299337/140‑1 (MQ=255)

                                                                                 ccGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTgg                                                 <  1:1252205/140‑1 (MQ=255)

                                                                                 ccGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTgg                                                 <  2:201382/140‑1 (MQ=255)

                                                                                 ccGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTgg                                                 <  1:355403/140‑1 (MQ=255)

                                                                                    tttCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTa                                              >  1:80069/1‑140 (MQ=255)

                                                                                      tCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATc                                            <  1:820502/140‑1 (MQ=255)

                                                                                      tCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATc                                            <  1:3668/140‑1 (MQ=255)

                                                                                         tGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGa                                         <  1:723173/140‑1 (MQ=255)

                                                                                            ggCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAag                                      <  2:931339/140‑1 (MQ=255)

                                                                                               aCTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAgg                                   >  1:27748/1‑140 (MQ=255)

                                                                                                 tGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGAt                                 >  2:55676/1‑140 (MQ=255)

                                                                                                   aaaGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCa                               >  2:268767/1‑140 (MQ=255)

                                                                                                   aaaGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCa                               <  2:886400/140‑1 (MQ=255)

                                                                                                    aaGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAg                              <  1:19025/140‑1 (MQ=255)

                                                                                                    aaGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAg                              <  1:1239778/140‑1 (MQ=255)

                                                                                                    aaGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAg                              <  2:1355920/140‑1 (MQ=255)

                                                                                                    aaGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAg                              <  1:986927/140‑1 (MQ=255)

                                                                                                     aGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGc                             <  1:860339/140‑1 (MQ=255)

                                                                                                        cAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGt                          <  2:18650/140‑1 (MQ=255)

                                                                                                        cAGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGt                          >  2:203157/1‑140 (MQ=255)

                                                                                                         aGCGGTAAGCAGCGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGtt                         <  1:319840/140‑1 (MQ=255)

                                                                                                                     cGAATTTTGCTCACCAATGCACATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGt             >  2:1078860/1‑140 (MQ=255)

TGGAGTGAGCAACTGGGTCTGGATATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGCCAGCGGTAAGCAGCGAATTTTGCTCACCAATGCGCATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACGCACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAAGCTA  >  NC_000913/3528885‑3529152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A