Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,607,656	G→A	A402A (GCG→GCA)	zntA →	Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,607,656	0	G	A	100.0%	159.8 / NA	45	A402A (GCG→GCA)	zntA	Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/22); total (23/22)

CGATTATGGCCGTCGCTCTGCTGGTGACGCTGGTGCCACCGCTGCTGTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCGGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAACCGGTACGCTGACCGTCGGTAAACCG  >  NC_000913/3607520‑3607788

cgATTATGGCCGTCGCTCTGCTGGTGACGCTGGTGCCACCGCTGCTGTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTgcagcg                                                                                                                                   >  1:755938/1‑140 (MQ=255)

cgATTATGGCCGTCGCTCTGCTGGTGACGCTGGTGCCACCGCTGCTGTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACACCTgcagcg                                                                                                                                   >  2:83368/1‑140 (MQ=255)

  aTTATGGCCGTCGCTCTGCTGGTGACGCTGGTGCCACCGCTGCTGTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGAt                                                                                                                                 <  2:12826/140‑1 (MQ=255)

           gTCGCTCTGCTGGTGACGCTGGTGCCACCGCTGCTGTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCgg                                                                                                                        >  1:314209/1‑140 (MQ=255)

            tCGCTCTGCTGGTGACGCTGGTGCCACCGCTGCTGTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCggg                                                                                                                       <  1:231729/140‑1 (MQ=255)

            tCGCTCTGCTGGTGACGCTGGTGCCACCGCTGCTGTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCggg                                                                                                                       <  2:130407/140‑1 (MQ=255)

            tCGCTCTGCTGGTGACGCTGGTGCCACCGCTGCTGTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCggg                                                                                                                       <  2:492391/140‑1 (MQ=255)

             cGCTCTGCTGGTGACGCTGGTGCCACCGCTGCTGTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGc                                                                                                                      >  2:295927/1‑140 (MQ=255)

                       gTGACGCTGGTGCCACCGCTGCTGTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTggcggcggc                                                                                                            >  2:12663/1‑140 (MQ=255)

                        tGACGCTGGTGCCACCGCTGCTGTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCa                                                                                                           <  2:589029/140‑1 (MQ=255)

                            gCTGGTGCCACCGCTGCTGTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAgcgc                                                                                                       >  2:1102154/1‑140 (MQ=255)

                              tGGTGCCACCGCTGCTGTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGcgt                                                                                                     >  1:1141311/1‑140 (MQ=255)

                               ggTGCCACCGCTGCTGTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGcgtc                                                                                                    <  2:755938/140‑1 (MQ=255)

                                       cGCTGCTGTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCg                                                                                            <  2:136217/140‑1 (MQ=255)

                                       cGCTGCTGTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCg                                                                                            <  1:417169/140‑1 (MQ=255)

                                           gctgTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGa                                                                                        >  2:532583/1‑140 (MQ=255)

                                            ctgTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGAt                                                                                       <  2:485349/140‑1 (MQ=255)

                                                       cAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAg                                                                            <  2:75759/140‑1 (MQ=255)

                                                        aGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAgc                                                                           <  2:184517/140‑1 (MQ=255)

                                                        aGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAgc                                                                           <  2:1166862/140‑1 (MQ=255)

                                                                   gTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAAc                                                                >  2:171438/1‑140 (MQ=255)

                                                                    tGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACa                                                               >  2:1414081/1‑140 (MQ=255)

                                                                     ggATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAg                                                              <  1:1174241/140‑1 (MQ=255)

                                                                           ataAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTg                                                           >  1:552661/1‑137 (MQ=255)

                                                                           ataAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTg                                                           <  2:552661/137‑1 (MQ=255)

                                                                             aaaGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCg                                                      <  2:469956/140‑1 (MQ=255)

                                                                                 ggCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGtt                                                  >  2:896535/1‑140 (MQ=255)

                                                                                  gCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTa                                                 >  2:333529/1‑140 (MQ=255)

                                                                                      aCGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCa                                             >  1:572043/1‑140 (MQ=255)

                                                                                           gctgctgATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTgg                                        >  2:68865/1‑140 (MQ=255)

                                                                                                  aTTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGa                                 >  2:917507/1‑140 (MQ=255)

                                                                                                      gCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATaaa                             >  2:104276/1‑140 (MQ=255)

                                                                                                         gCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGGAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAAcc                          <  1:1052505/140‑1 (MQ=255)

                                                                                                         gCCCGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAAcc                          >  1:826684/1‑140 (MQ=255)

                                                                                                            cGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAACCGGt                       >  1:1240954/1‑140 (MQ=255)

                                                                                                            cGTGTGCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAACCGGt                       <  1:295927/140‑1 (MQ=255)

                                                                                                               gtgCGTTAGTTATCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAACCGGTACg                    <  1:944056/140‑1 (MQ=255)

                                                                                                                    ttagttaTCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAACCGGTACGCTGAc               <  2:1124428/140‑1 (MQ=255)

                                                                                                                    ttagttaTCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAACCGGTACGCTGAc               <  1:1102154/140‑1 (MQ=255)

                                                                                                                    ttagttaTCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAACCGGTACGCTGAc               <  1:1414081/140‑1 (MQ=255)

                                                                                                                    ttagttaTCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAACCGGTACGCTGAc               <  1:896535/140‑1 (MQ=255)

                                                                                                                        ttaTCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAACCGGTACGCTGACCGTc           >  1:434786/1‑140 (MQ=255)

                                                                                                                          aTCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAGAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAACCGGTACGCTGAc               >  2:1312520/1‑134 (MQ=255)

                                                                                                                          aTCTCAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAACCGGTACGCTGAc               <  1:1312520/134‑1 (MQ=255)

                                                                                                                            ctcAACGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAACCGGTACGCTGACCGTCGGTa       >  1:106617/1‑140 (MQ=255)

                                                                                                                               aaCGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAACCGGTACGCTGACCGTCGGTAAAc    >  1:457028/1‑140 (MQ=255)

                                                                                                                               aaCGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAACCGGTACGCTGACCGTCGGTAAAc    >  2:19007/1‑140 (MQ=255)

                                                                                                                                 cGCCTGCAGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAACCGGTACGCTGACCGTCGGTAAACcg  >  1:783924/1‑140 (MQ=255)

CGATTATGGCCGTCGCTCTGCTGGTGACGCTGGTGCCACCGCTGCTGTTTGCCGCCAGCTGGCAGGAGTGGATTTATAAAGGGCTGACGCTGCTGCTGATTGGCTGCCCGTGTGCGTTAGTTATCTCAACGCCTGCGGCGATTACCTCCGGGCTGGCGGCGGCAGCGCGTCGTGGGGCGTTGATTAAAGGCGGAGCGGCGCTGGAACAGCTGGGTCGTGTTACTCAGGTGGCGTTTGATAAAACCGGTACGCTGACCGTCGGTAAACCG  >  NC_000913/3607520‑3607788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A