Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,612,265	T→C	V134A (GTT→GCT)	yhhT →	putative transporter YhhT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,612,265	0	T	C	100.0%	208.8 / NA	59	V134A (GTT→GCT)	yhhT	putative transporter YhhT
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (30/29); total (30/29)

ATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGAATAATCCA  >  NC_000913/3612131‑3612401

aTTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCt                                                                                                                                     <  1:167147/140‑1 (MQ=255)

  ttATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTc                                                                                                                                   >  2:384139/1‑140 (MQ=255)

   tATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTca                                                                                                                                  >  2:270668/1‑140 (MQ=255)

        ctATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACcaca                                                                                                                             <  1:430269/140‑1 (MQ=255)

        ctATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACcaca                                                                                                                             <  1:225927/140‑1 (MQ=255)

         tATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAg                                                                                                                            >  2:26188/1‑140 (MQ=255)

                ccGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTcac                                                                                                                                 <  2:316084/128‑1 (MQ=255)

                ccGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTcac                                                                                                                                 >  1:316084/1‑128 (MQ=255)

                   aaGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGAc                                                                                                                  <  2:169705/140‑1 (MQ=255)

                       ttAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCggg                                                                                                              <  1:440339/140‑1 (MQ=255)

                       ttAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCggg                                                                                                              <  1:270668/140‑1 (MQ=255)

                          ataaGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACcaca                                                                                                                             <  2:612644/122‑1 (MQ=255)

                          ataaGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACcaca                                                                                                                             >  1:612644/1‑122 (MQ=255)

                                aGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCggg                                                                                                     >  1:540196/1‑140 (MQ=255)

                                aGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCggg                                                                                                     >  1:203063/1‑140 (MQ=255)

                                    gACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCaa                                                                                                 >  2:559906/1‑140 (MQ=255)

                                     aCGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAAt                                                                                                >  2:620900/1‑140 (MQ=255)

                                       gcgcAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATgg                                                                                              >  2:60535/1‑140 (MQ=255)

                                                 tttAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCt                                                                                    >  1:837918/1‑140 (MQ=255)

                                                 tttAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCt                                                                                    <  2:722447/140‑1 (MQ=255)

                                                     aaTTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTg                                                                                <  1:26188/140‑1 (MQ=255)

                                                                aTGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGAc                                                                     <  2:75540/140‑1 (MQ=255)

                                                                  gTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCg                                                                   <  2:837918/140‑1 (MQ=255)

                                                                   ttGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGt                                                                  <  2:203063/140‑1 (MQ=255)

                                                                     gCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAg                                                                >  1:701506/1‑140 (MQ=255)

                                                                         tttCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGtttt                                                            >  1:424172/1‑140 (MQ=255)

                                                                           tCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGAGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTa                                                          >  1:142908/1‑140 (MQ=255)

                                                                            cTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTAt                                                         <  2:540196/140‑1 (MQ=255)

                                                                            cTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTAt                                                         <  1:620900/140‑1 (MQ=255)

                                                                                aTTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGC‑TTTGCTGGTGATGACCGTAGTTTTTATGCTGt                                                    >  2:515353/1‑140 (MQ=255)

                                                                                       tatGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAg                                              >  1:211448/1‑140 (MQ=255)

                                                                                          gTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTgc                                           >  2:189100/1‑140 (MQ=255)

                                                                                           tCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTgcg                                          >  2:178180/1‑140 (MQ=255)

                                                                                             gCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGcc                                        <  1:178180/140‑1 (MQ=255)

                                                                                               cGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCAc                                      <  2:208156/140‑1 (MQ=255)

                                                                                               cGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCAc                                      <  1:409606/140‑1 (MQ=255)

                                                                                                      aaTGCTGCAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCtt                               >  1:599692/1‑140 (MQ=255)

                                                                                                            gcAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACaaaa                         >  2:792386/1‑140 (MQ=255)

                                                                                                             cAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCAc                                      <  1:526980/126‑1 (MQ=255)

                                                                                                             cAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCAc                                      >  2:526980/1‑126 (MQ=255)

                                                                                                             cAGCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAAt                        >  2:124443/1‑140 (MQ=255)

                                                                                                               gCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGc                      <  2:72656/140‑1 (MQ=255)

                                                                                                               gCGGATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGc                      <  2:383214/140‑1 (MQ=255)

                                                                                                                  gATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGtt                   >  1:793956/1‑140 (MQ=255)

                                                                                                                  gATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGtt                   <  1:28115/140‑1 (MQ=255)

                                                                                                                  gATGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGtt                   >  1:72664/1‑140 (MQ=255)

                                                                                                                    tGGACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGtttt                 >  1:28024/1‑140 (MQ=255)

                                                                                                                     ggACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTg                >  1:227958/1‑140 (MQ=255)

                                                                                                                     ggACTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTg                >  1:75906/1‑140 (MQ=255)

                                                                                                                       aCTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTgcg              <  2:211448/140‑1 (MQ=255)

                                                                                                                       aCTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTgcg              <  1:147092/140‑1 (MQ=255)

                                                                                                                       aCTCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGAGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTgcg              <  2:142908/140‑1 (MQ=255)

                                                                                                                         tCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCt            <  2:28024/140‑1 (MQ=255)

                                                                                                                         tCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCt            <  1:206713/140‑1 (MQ=255)

                                                                                                                         tCGGAAAAAGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCt            <  1:124443/140‑1 (MQ=255)

                                                                                                                             aaaaaGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGaat        >  2:795398/1‑140 (MQ=255)

                                                                                                                                 aGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGaa         >  1:827434/1‑135 (MQ=255)

                                                                                                                                 aGTGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGaa         <  2:827434/135‑1 (MQ=255)

                                                                                                                                   tGGCTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGAATAATCca  >  2:847695/1‑140 (MQ=255)

ATTTATCTCTATGCTGCCGAAGTTTAATAAGGAGCTGACGCGCAAACTTTTTAAATTGCAGGAGATGTTGCCTTTTCTTAATTTGCATATGTCGCCGGAGCGAATGCTGCAGCGGATGGACTCGGAAAAAGTGGTTACCTTCACCACAGCGCTAATGACCGGGCTTTCCGGGGCAATGGCGAGCGTGCTTTTGCTGGTGATGACCGTAGTTTTTATGCTGTTTGAAGTGCGCCACGTCCCTTACAAAATGCGTTTTGCGCTGAATAATCCA  >  NC_000913/3612131‑3612401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A