Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,694,686	(C)_6→7	coding (551/2619 nt)	bcsA ←	cellulose synthase catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,694,680	1	.	C	95.5%	231.2 / 3.0	66	coding (557/2619 nt)	bcsA	cellulose synthase catalytic subunit
Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (32/31); total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CACACCAGGCTGACCGGATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TA‑CCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATG  >  NC_000913/3694542‑3694813

cacaCCAGGCTGACCGGATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TA‑c                                                                                                                                      <  1:775591/140‑1 (MQ=255)

cacaCCAGGCTGACCGGATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TA‑c                                                                                                                                      <  1:536395/140‑1 (MQ=255)

cacaCCAGGCTGACCGGATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TA‑c                                                                                                                                      >  2:1179643/1‑140 (MQ=255)

cacaCCAGGCTGACCGGATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TA‑c                                                                                                                                      >  2:1358090/1‑140 (MQ=255)

     cAGGCTGACCGGATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TA‑cccccc                                                                                                                                 >  1:945979/1‑140 (MQ=255)

     cAGGCTGACCGGATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TA‑cccccc                                                                                                                                 >  2:809165/1‑140 (MQ=255)

          tGACCGGATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACTAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TA‑cccccccacag                                                                                                                            <  2:1230873/140‑6 (MQ=255)

          tGACCGGATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TA‑cccccccacag                                                                                                                            <  1:100280/140‑6 (MQ=255)

          tGACCGGATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TA‑cccccccacag                                                                                                                            <  2:988408/140‑6 (MQ=255)

            aCCGGATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCAcagca                                                                                                                           <  2:184542/140‑1 (MQ=255)

             ccGGATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCAcagcag                                                                                                                          >  1:350165/1‑140 (MQ=255)

               ggATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAgcagca                                                                                                                        >  1:963995/1‑140 (MQ=255)

                gATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCAt                                                                                                                       <  1:1179643/140‑1 (MQ=255)

                gATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCAt                                                                                                                       <  1:1324351/140‑1 (MQ=255)

                 aTCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGTTATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATc                                                                                                                      >  1:1188670/1‑140 (MQ=255)

                 aTCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATc                                                                                                                      >  2:863716/1‑140 (MQ=255)

                  tCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCa                                                                                                                     <  2:842797/140‑1 (MQ=255)

                  tCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCa                                                                                                                     <  1:1063454/140‑1 (MQ=255)

                  tCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCa                                                                                                                     <  2:320316/140‑1 (MQ=255)

                  tCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGACATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCa                                                                                                                     <  1:856757/140‑1 (MQ=255)

                        cAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGa                                                                                                               <  1:207147/140‑1 (MQ=255)

                           ttCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATaa                                                                                                            >  1:728901/1‑140 (MQ=255)

                              aGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACt                                                                                                         <  2:776784/140‑1 (MQ=255)

                                cGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTgc                                                                                                       >  2:550545/1‑140 (MQ=255)

                                    gagGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCa                                                                                                   <  2:71715/140‑1 (MQ=255)

                                    gagGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCa                                                                                                   <  1:843025/140‑1 (MQ=255)

                                    gagGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCa                                                                                                   <  1:809165/140‑1 (MQ=255)

                                     agGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAg                                                                                                  >  2:85556/1‑140 (MQ=255)

                                     agGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAg                                                                                                  >  2:985286/1‑140 (MQ=255)

                                            aCGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTa                                                                                           >  2:1024991/1‑140 (MQ=255)

                                               ccaccaGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAAc                                                                                        >  2:10384/1‑140 (MQ=255)

                                                 accaGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGCTTC‑CCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACgg                                                                                      <  1:195435/140‑1 (MQ=255)

                                                      atatAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAg                                                                                 <  1:1024991/140‑1 (MQ=255)

                                                      atatAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAg                                                                                 <  1:129210/140‑1 (MQ=255)

                                                               cAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGa                                                                        >  1:1034868/1‑140 (MQ=255)

                                                                aaGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGAt                                                                       >  2:864072/1‑140 (MQ=255)

                                                                aaGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TA‑CCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGAtt                                                                      >  1:616944/1‑140 (MQ=255)

                                                                 aGAAACGGTCAGCGGCAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGAtt                                                                      <  1:595352/140‑1 (MQ=255)

                                                                 aGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTGAACGGCTGAGTAACg                                                                            >  2:1286980/1‑134 (MQ=255)

                                                                 aGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACg                                                                            <  1:1286980/134‑1 (MQ=255)

                                                                 aGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGAtt                                                                      >  1:498389/1‑140 (MQ=255)

                                                                 aGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGAtt                                                                      <  2:269709/140‑1 (MQ=255)

                                                                            gCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAAt                                                           <  1:864072/140‑1 (MQ=255)

                                                                               aCAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAg                                                        <  1:1358090/140‑1 (MQ=255)

                                                                                  gCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCga                                                     >  2:25497/1‑140 (MQ=255)

                                                                                   cacaATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGTTGAGTAACGCAGATTAACGCCAGAATCAGCgag                                                    >  1:1062317/1‑140 (MQ=255)

                                                                                   cacaATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCgag                                                    >  2:157832/1‑140 (MQ=255)

                                                                                   cacaATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCgag                                                    >  1:324272/1‑140 (MQ=255)

                                                                                   cacaATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCgag                                                    <  2:324272/140‑1 (MQ=255)

                                                                                   cacaATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TA‑CCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCgaga                                                   <  2:616944/140‑1 (MQ=255)

                                                                                    acaATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACACCAGAATCAGCgaga                                                   <  1:1274743/140‑1 (MQ=255)

                                                                                        tCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGt                                               <  1:863716/140‑1 (MQ=255)

                                                                                          aaCATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGa                                             >  2:1125993/1‑140 (MQ=255)

                                                                                          aaCATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGa                                             <  1:550545/140‑1 (MQ=255)

                                                                                               tAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACgatg                                        <  2:1034868/140‑1 (MQ=255)

                                                                                                 gcgcCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACgatgat                                      >  1:271132/1‑140 (MQ=255)

                                                                                                  cgcCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCga                                                     <  1:1138200/124‑1 (MQ=255)

                                                                                                  cgcCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCga                                                     >  2:1138200/1‑124 (MQ=255)

                                                                                                  cgcCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACgatga                                       <  1:754163/138‑1 (MQ=255)

                                                                                                  cgcCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACgatga                                       >  2:754163/1‑138 (MQ=255)

                                                                                                  cgcCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATa                                     >  2:1303572/1‑140 (MQ=255)

                                                                                                  cgcCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATa                                     >  1:415579/1‑140 (MQ=255)

                                                                                                   gcCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATAc                                    <  1:1407182/140‑1 (MQ=255)

                                                                                                   gcCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATAc                                    <  2:1150736/140‑1 (MQ=255)

                                                                                                    ccGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATAcc                                   >  1:532608/1‑140 (MQ=255)

                                                                                                       agaAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAg                                >  2:1244910/1‑140 (MQ=255)

                                                                                                            cgcCCCGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCa                           >  1:152997/1‑140 (MQ=255)

                                                                                                                 cGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACgatg                                        <  1:1074782/122‑1 (MQ=255)

                                                                                                                 cGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACgatg                                        >  2:1074782/1‑122 (MQ=255)

                                                                                                                 cGGCATCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAAccgc                      >  1:383894/1‑140 (MQ=255)

                                                                                                                      tCCGCCGTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCgcgc                 <  2:1188670/140‑1 (MQ=255)

                                                                                                                        cgccgTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGccc               <  1:20310/140‑1 (MQ=255)

                                                                                                                          ccgTCCGATCGGCCC‑TCCCCCCCCACAGCAGCATCAGGAAGAAAACCGGCCCCAGCGGTTTAACCGGCTGAGTAACGAAGATTAACGCCAGAACCAGCGAGAGGGGGACGATGATCCCGAGGATCAACCGCCGCGCCCCg             <  2:702263/140‑1 (MQ=255)

                                                                                                                            gTACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGc           <  2:152997/140‑1 (MQ=255)

                                                                                                                             tACGATCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGcc          >  2:685153/1‑139 (MQ=255)

                                                                                                                                  tCAGCGC‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCaa     >  1:949104/1‑140 (MQ=255)

                                                                                                                                     gcgc‑TACCCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATg  >  1:702263/1‑140 (MQ=255)

CACACCAGGCTGACCGGATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGC‑TA‑CCCCCCACAGCAGCATCAGGAAGATAAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATG  >  NC_000913/3694542‑3694813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A