Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,032,963	T→C	C158R (TGT→CGT)	yigZ →	IMPACT family member YigZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,032,963	0	T	C	100.0%	284.4 / NA	77	C158R (TGT→CGT)	yigZ	IMPACT family member YigZ
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (49/28); total (49/28)

GGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGTGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCATTGCAATTGTTAGCGATTG  >  NC_000913/4032826‑4033098

ggTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAgcgt                                                                                                                                       >  1:990124/1‑140 (MQ=255)

     gTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACgg                                                                                                                                  >  1:40960/1‑140 (MQ=255)

       gAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACgg                                                                                                                                  <  2:154049/138‑1 (MQ=255)

       gAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACgg                                                                                                                                  >  1:154049/1‑138 (MQ=255)

       gAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCa                                                                                                                                <  2:948063/140‑1 (MQ=255)

       gAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCa                                                                                                                                <  2:313594/140‑1 (MQ=255)

          aGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCaaaa                                                                                                                             >  1:1282200/1‑140 (MQ=255)

            cGTATGGCGGCGGCGGGAATCAGGCGCTGCGCCAGCTAACGACCCAACGCCAGACGCCATTAACCGAATATACATTGCAATGAGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCCGCGTGACGGCAAAAtt                                                                                                                           >  2:1239375/1‑140 (MQ=255)

              tATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTAt                                                                                                                         <  2:1282200/140‑1 (MQ=255)

                  gcggcggcgTGAATCAGGCGCTGCGCCAGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAAc                                                                                                                     >  1:1175659/1‑140 (MQ=255)

                      cggcgTGAATCAGGCGCTGCGCCAGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTg                                                                                                                 >  2:254262/1‑140 (MQ=255)

                             aaTCAGGCGCTGCGCCAGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTAt                                                                                                                         >  1:912996/1‑125 (MQ=255)

                             aaTCAGGCGCTGCGCCAGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTAt                                                                                                                         <  2:912996/125‑1 (MQ=255)

                                  ggCGCTGCGCCAGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCAt                                                                                                     <  2:1228748/140‑1 (MQ=255)

                                         cgcCAGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTct                                                                                              >  1:1317243/1‑140 (MQ=255)

                                             aGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTctgctg                                                                                          <  2:40960/140‑1 (MQ=255)

                                             aGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTctgctg                                                                                          >  1:472817/1‑140 (MQ=255)

                                                   cGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTg                                                                                    >  1:574051/1‑140 (MQ=255)

                                                                gACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCcggcgg                                                                       >  1:216119/1‑140 (MQ=255)

                                                                gACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCcggcgg                                                                       >  1:886161/1‑140 (MQ=255)

                                                                gACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCcggcgg                                                                       >  2:1352502/1‑140 (MQ=255)

                                                                gACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCcggcgg                                                                       >  1:1138465/1‑140 (MQ=255)

                                                                     cATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGaaa                                                                  >  1:1412694/1‑140 (MQ=255)

                                                                     cATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGaaa                                                                  >  2:1355900/1‑140 (MQ=255)

                                                                                      ttGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGc                                                 >  1:159719/1‑140 (MQ=255)

                                                                                      ttGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGc                                                 >  1:564264/1‑140 (MQ=255)

                                                                                      ttGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGc                                                 >  2:141145/1‑140 (MQ=255)

                                                                                       tGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCa                                                >  2:794073/1‑140 (MQ=255)

                                                                                        gttaactgAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCaa                                               >  2:247578/7‑140 (MQ=255)

                                                                                        gCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCaa                                               >  2:1346614/1‑140 (MQ=255)

                                                                                        gCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCaa                                               >  2:250961/1‑140 (MQ=255)

                                                                                         cAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCaaa                                              >  2:951711/1‑140 (MQ=255)

                                                                                         cAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCaaa                                              >  1:1243972/1‑140 (MQ=255)

                                                                                         cAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCaaa                                              >  2:914500/1‑140 (MQ=255)

                                                                                            tgtgAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCt                                           >  1:617619/1‑140 (MQ=255)

                                                                                            tgtgAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCt                                           >  1:1245871/1‑140 (MQ=255)

                                                                                             gtgAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATAAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTg                                          >  2:967508/1‑140 (MQ=255)

                                                                                              tgAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTgg                                         >  2:1041023/1‑140 (MQ=255)

                                                                                               gAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGc                                        >  2:763021/1‑140 (MQ=255)

                                                                                                    tcatcaGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGAtt                                   <  2:574051/140‑1 (MQ=255)

                                                                                                      atcaGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGAtttt                                 >  2:280949/1‑140 (MQ=255)

                                                                                                      atcaGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGAtttt                                 <  2:990124/140‑1 (MQ=255)

                                                                                                      atcaGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGAtttt                                 <  1:1041023/140‑1 (MQ=255)

                                                                                                       tcaGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTa                                >  2:190873/1‑140 (MQ=255)

                                                                                                        caGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAg                               >  2:661842/1‑140 (MQ=255)

                                                                                                        caGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAg                               >  1:921785/1‑140 (MQ=255)

                                                                                                        caGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAg                               >  1:704348/1‑140 (MQ=255)

                                                                                                         aGTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGc                              <  2:1317243/140‑1 (MQ=255)

                                                                                                          gTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGcc                             <  1:230476/140‑1 (MQ=255)

                                                                                                          gTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGcc                             >  2:194159/1‑140 (MQ=255)

                                                                                                          gTTAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGcc                             >  1:593593/1‑140 (MQ=255)

                                                                                                           ttAACCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCg                            <  1:141145/140‑1 (MQ=255)

                                                                                                             aaCCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTg                          <  1:763021/140‑1 (MQ=255)

                                                                                                              aCCGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTgg                         >  1:733554/1‑140 (MQ=255)

                                                                                                                cGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGtt                       >  1:327055/1‑140 (MQ=255)

                                                                                                                cGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGtt                       <  1:1355900/140‑1 (MQ=255)

                                                                                                                cGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGtt                       <  1:250961/140‑1 (MQ=255)

                                                                                                                cGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGtt                       <  1:1352502/140‑1 (MQ=255)

                                                                                                                cGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGtt                       <  1:914500/140‑1 (MQ=255)

                                                                                                                cGGCATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATAAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGtt                       <  1:967508/140‑1 (MQ=255)

                                                                                                                   cATTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCAt                    >  1:1088002/1‑140 (MQ=255)

                                                                                                                    aTTGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCAtt                   >  1:1156104/1‑140 (MQ=255)

                                                                                                                     ttGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCATTg                  >  1:540730/1‑140 (MQ=255)

                                                                                                                     ttGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCATTg                  <  2:886161/140‑1 (MQ=255)

                                                                                                                     ttGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCATTg                  <  2:472817/140‑1 (MQ=255)

                                                                                                                     ttGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCATTg                  <  2:1412694/140‑1 (MQ=255)

                                                                                                                     ttGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCATTg                  >  2:1168491/1‑140 (MQ=255)

                                                                                                                      tGAAGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCATTGc                 <  1:247578/140‑1 (MQ=255)

                                                                                                                        aaGCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCATTGCaa               >  2:1142416/1‑140 (MQ=255)

                                                                                                                          gCGTTGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCATTGCAAtt             >  1:100990/1‑140 (MQ=255)

                                                                                                                             ttGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGtt                       >  1:1276884/1‑127 (MQ=255)

                                                                                                                             ttGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGtt                       <  2:1276884/127‑1 (MQ=255)

                                                                                                                             ttGCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCATTGCAATTGtt          >  2:477266/1‑140 (MQ=255)

                                                                                                                               gCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCATTGCAATTGTTAg        <  2:852398/140‑1 (MQ=255)

                                                                                                                               gCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCATTGCAATTGTTAg        >  1:475532/1‑140 (MQ=255)

                                                                                                                               gCTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCATTGCAATTGTTAg        >  1:482140/1‑140 (MQ=255)

                                                                                                                                cTGGGGCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGTCGTGGTTCATTGCAATTGTTAGc       <  2:365155/140‑1 (MQ=255)

                                                                                                                                    ggCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCATTGCAATTGTTAGCGAtt   <  2:10727/140‑1 (MQ=255)

                                                                                                                                     gCAGCGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCATTGCAATTGTTAGCGATTg  <  2:1138465/140‑1 (MQ=255)

GGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAACGACCCAACGCAAGACGCCATTAACCGAATATACTTTGCAATGTGAATATCATCAGTTAACCGGCATTGAAGCGTTGCTGGGGCAGTGTGACGGCAAAATTATCAACAGTGATTATCAGGCATTCGTTCTGCTGCGGGTGGCGCTTCCGGCGGCGAAAGTGGCTGAATTTTCCGCAAAGCTGGCGGATTTTAGCCGTGGTTCATTGCAATTGTTAGCGATTG  >  NC_000913/4032826‑4033098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A