Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,300,240	C→T	R264H (CGT→CAT)	mdtP ←	putative multidrug efflux pump outer membrane channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,300,240	0	C	T	100.0%	426.5 / NA	119	R264H (CGT→CAT)	mdtP	putative multidrug efflux pump outer membrane channel
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (70/49); total (70/49)

Only 100 of 124 total aligned reads displayed.

ACCAGCGCATGGCTTGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTT  >  NC_000913/4300103‑4300376

aCCAGCGCATGGCTTGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAatgcatgc                                                                                                                                        >  1:914771/1‑140 (MQ=255)

aCCAGCGCATGGCTTGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAatgcatgc                                                                                                                                        >  1:935473/1‑140 (MQ=255)

     cgcATGGCTTGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGGGAGTGTCGCCGGAATGCCAGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCGATCAATGCATGCAgaga                                                                                                                                   >  2:1259386/1‑140 (MQ=255)

     cgcATGGCTTGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCGgaga                                                                                                                                   >  2:122704/1‑140 (MQ=255)

     cgcATGGCTTGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAgaga                                                                                                                                   >  1:436962/1‑140 (MQ=255)

     cgcATGGCTTGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAgaga                                                                                                                                   >  1:331155/1‑140 (MQ=255)

     cgcATGGCTTGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAgaga                                                                                                                                   >  2:475838/1‑140 (MQ=255)

          ggCTTGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATtctc                                                                                                                              >  1:1263060/1‑140 (MQ=255)

              tGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCACGCGCCAATCAATGCATGCAGAGATTCTCGCGt                                                                                                                          >  1:884333/1‑140 (MQ=255)

               gCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGtt                                                                                                                         >  2:817588/1‑140 (MQ=255)

               gCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGt                                                                                                                          >  1:792722/1‑139 (MQ=255)

                     ccGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTTCCGCGCCAATCAATGCATGCAGAGATTCTcg                                                                                                                             >  2:205583/1‑130 (MQ=255)

                     ccGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTcg                                                                                                                             <  1:205583/130‑1 (MQ=255)

                      cGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGtta                                                                                                                  >  1:544918/1‑138 (MQ=255)

                      cGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGa                                                                                                                  >  2:1077929/1‑140 (MQ=255)

                        ggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGAtt                                                                                                                <  2:1069542/140‑1 (MQ=255)

                        ggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGAtt                                                                                                                >  2:1216036/1‑140 (MQ=255)

                           gTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGc                                                                                                             <  1:1114766/140‑1 (MQ=255)

                              tGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAACGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCt                                                                                                          >  1:598816/1‑140 (MQ=255)

                                gCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCttt                                                                                                        <  1:118104/140‑1 (MQ=255)

                                     cAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACgg                                                                                                   >  2:670307/1‑140 (MQ=255)

                                      aaCTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGc                                                                                                  <  1:654652/140‑1 (MQ=255)

                                      aaCTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGc                                                                                                  <  1:817588/140‑1 (MQ=255)

                                        cTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGcaa                                                                                                >  1:578255/1‑140 (MQ=255)

                                             aaGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAAtt                                                                                           >  1:371074/1‑140 (MQ=255)

                                                  agTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGttt                                                                                      >  2:662582/1‑140 (MQ=255)

                                                  agTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGttt                                                                                      >  1:656825/1‑140 (MQ=255)

                                                  agTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGttt                                                                                      >  1:1108423/1‑140 (MQ=255)

                                                  agTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGttt                                                                                      >  2:1105843/1‑140 (MQ=255)

                                                  agTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGttt                                                                                      >  1:836827/1‑140 (MQ=255)

                                                  agTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGttt                                                                                      >  1:239036/1‑140 (MQ=255)

                                                  agTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGttt                                                                                      >  2:1312297/1‑140 (MQ=255)

                                                  agTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGttt                                                                                      >  2:1041946/1‑140 (MQ=255)

                                                  agTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGttt                                                                                      >  2:1077495/1‑140 (MQ=255)

                                                   gtgtCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAAt                                                                                            <  2:193186/133‑1 (MQ=255)

                                                   gtgtCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTa                                                                                     >  1:669586/1‑140 (MQ=255)

                                                   gtgtCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTa                                                                                     >  1:1073651/1‑140 (MQ=255)

                                                     gtCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATc                                                                                   >  1:617187/1‑140 (MQ=255)

                                                     gtCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATc                                                                                   >  2:272848/1‑140 (MQ=255)

                                                     gtCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATc                                                                                   >  2:1236712/1‑140 (MQ=255)

                                                     gtCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATc                                                                                   >  2:884500/1‑140 (MQ=255)

                                                      tCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCg                                                                                  >  2:810672/1‑140 (MQ=255)

                                                      tCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCg                                                                                  >  1:1151047/1‑140 (MQ=255)

                                                      tCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCg                                                                                  >  1:1297722/1‑140 (MQ=255)

                                                       cGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGa                                                                                 >  1:688448/1‑140 (MQ=255)

                                                       cGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGa                                                                                 <  1:1077495/140‑1 (MQ=255)

                                                        gCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGAc                                                                                <  1:1264131/140‑1 (MQ=255)

                                                             aaTGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCa                                                                           <  1:136588/140‑1 (MQ=255)

                                                             aaTGCCGGTCTGGACTTGCGGCAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCa                                                                           <  2:1286306/140‑1 (MQ=255)

                                                                    gTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCtg                                                                    <  2:1073651/140‑1 (MQ=255)

                                                                    gTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTAGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCtg                                                                    >  1:1406731/1‑140 (MQ=255)

                                                                         gACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGccc                                                               >  1:476988/1‑140 (MQ=255)

                                                                          aCTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCcg                                                              >  1:682396/1‑140 (MQ=255)

                                                                           cTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCcgc                                                             >  1:1255704/1‑140 (MQ=255)

                                                                            ttGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCcgcg                                                            <  2:385847/140‑1 (MQ=255)

                                                                            ttGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCcgcg                                                            <  1:1216036/140‑1 (MQ=255)

                                                                             tGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCcgcgc                                                           <  2:682396/140‑1 (MQ=255)

                                                                              gCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCcgcgcg                                                          <  2:239036/140‑1 (MQ=255)

                                                                               cGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCgcgcgc                                                         >  1:1164975/1‑140 (MQ=255)

                                                                                   aaTGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGcc                                                        <  2:100979/137‑1 (MQ=255)

                                                                                   aaTGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGcc                                                        >  1:100979/1‑137 (MQ=255)

                                                                                         gccGGTCTGATTTCCGGCATATCCCTGGCTCCCGCGCCAATCAATGCATGCAGAGCTTCCCGCGTTTCGGGGATTTCCCCTTTGACGGCAAAAATTTGTTTATCGACCGCCAGAATCTGTGCCCACGCGCCGTGGAAAgg                                               >  2:1011216/2‑140 (MQ=255)

                                                                                           cGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCa                                             <  2:617187/140‑1 (MQ=255)

                                                                                            ggTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCAc                                            >  2:644718/1‑140 (MQ=255)

                                                                                               cTGATCTCCGGCATATCGCTGGCTCGCGCGCCAATCAATGCATGCGGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTg                                         <  1:122704/140‑1 (MQ=255)

                                                                                               cTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACtta                                         <  2:913232/140‑2 (MQ=255)

                                                                                               cTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTg                                         <  2:1297722/140‑1 (MQ=255)

                                                                                               cTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTg                                         <  2:588632/140‑1 (MQ=255)

                                                                                               cTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTg                                         <  1:1236712/140‑1 (MQ=255)

                                                                                               cTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTg                                         <  2:1263060/140‑1 (MQ=255)

                                                                                               cTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTg                                         <  1:475838/140‑1 (MQ=255)

                                                                                               cTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTg                                         <  1:997404/140‑1 (MQ=255)

                                                                                               cTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGACACTTg                                         <  1:659200/140‑1 (MQ=255)

                                                                                                 gATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTgcg                                       <  2:1133046/140‑1 (MQ=255)

                                                                                                       cGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTg                                         >  1:375645/1‑132 (MQ=255)

                                                                                                       cGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTg                                         <  2:375645/132‑1 (MQ=255)

                                                                                                       cGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCa                                 >  2:1282395/1‑140 (MQ=255)

                                                                                                       cGGCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCa                                 >  2:549266/1‑140 (MQ=255)

                                                                                                         gCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGa                               >  1:1187884/1‑140 (MQ=255)

                                                                                                         gCATATCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGa                               <  1:965591/140‑1 (MQ=255)

                                                                                                            tatCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCg                            >  2:400039/1‑140 (MQ=255)

                                                                                                              tCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTg                          >  2:1259495/1‑140 (MQ=255)

                                                                                                              tCGCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTg                          <  2:578255/140‑1 (MQ=255)

                                                                                                                gCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTgcg                        <  1:827294/140‑1 (MQ=255)

                                                                                                                gCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTgcg                        <  1:493449/140‑1 (MQ=255)

                                                                                                                gCTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTgcg                        <  1:810672/140‑1 (MQ=255)

                                                                                                                 cTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTgcgc                       >  2:261998/1‑140 (MQ=255)

                                                                                                                 cTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTgcgc                       >  1:216223/1‑140 (MQ=255)

                                                                                                                 cTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTgcgc                       >  1:1391986/1‑140 (MQ=255)

                                                                                                                 cTGGCTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTgcgc                       >  1:681548/1‑140 (MQ=255)

                                                                                                                     cTCCCGCGCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACt                   >  1:219045/1‑140 (MQ=255)

                                                                                                                         cgcgCCAATCAATGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTAc               >  2:1245131/1‑140 (MQ=255)

                                                                                                                              caatcaatGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTgg          <  2:560623/140‑1 (MQ=255)

                                                                                                                              caatcaatGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTgg          <  2:71909/140‑1 (MQ=255)

                                                                                                                              caatcaatGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTgg          <  2:688448/140‑1 (MQ=255)

                                                                                                                              caatcaatGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTgg          <  1:1259495/140‑1 (MQ=255)

                                                                                                                                atcaatGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTg        >  2:683373/1‑140 (MQ=255)

                                                                                                                                atcaatGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTg        <  1:589515/140‑1 (MQ=255)

                                                                                                                                   aatGCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTgcgc     >  1:1317525/1‑140 (MQ=255)

                                                                                                                                      gCATGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCttt  <  1:299915/140‑1 (MQ=255)

ACCAGCGCATGGCTTGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTT  >  NC_000913/4300103‑4300376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A